Skip to main content
Log in

Detection of Enterobacterales resistant to polymyxins using Rapid Polymyxins NP test

  • Clinica Microbiology - Short Communication
  • Published:
Brazilian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Two hundred isolates of Enterobacterales were tested by Rapid Polymyxins NP for the detection of polymyxin resistance and compared to the reference test broth microdilution (BMD). The sensitivity and specificity of the NP test were 98% and the results are faster than the BMD, decreasing from approximately 24 to 2 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Baughman RP (2009) The use of carbapenems in the treatment of serious infections. J Intensive Care Med 24(4):230–241

    Article  PubMed  Google Scholar 

  2. Leung LM, Cooper VS, Rasko DA, Guo Q, Pacey MP, McElheny CL, Mettus RT, Yoon SH, Goodlett DR, Ernst RK, Doi Y (2017) Structural modification of LPS in colistin-resistant, KPC-producing Klebsiella pneumoniae. J Antimicrob Chemother 72(11):3035–3042

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Velkov T, Deris ZZ, Huang ZX et al (2013) Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae. Innate Immun 20(4):350–363

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J (2016) Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infec Dis 16(2):161–168

    Article  CAS  Google Scholar 

  5. Jayol A, Dubois V, Poirel L, Nordmann P (2016) Rapid detection of polymyxin-resistant Enterobacteriaceae from blood cultures. J Clin Microbiol 54(9):2273–2277

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Nordmann P, Poirel L (2016a) Plasmid-mediated colistin resistance: an additional antibiotic resistance menace. Clin Microbiol Infect 22(5):398–400

    Article  CAS  PubMed  Google Scholar 

  7. Nordmann P, Jayol A, Poirel L (2016b) Rapid detection of polymyxin resistance in Enterobacteriaceae. Emerg Infect Dis 22(6):1038–1043

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Rozales FP, Ribeiro VB, Magagnin CM, Pagano M, Lutz L, Falci DR, Machado A, Barth AL, Zavascki AP (2014) Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil. Int J Infec Dis 25:79–81

    Article  Google Scholar 

  9. Lentz SA, de Lima-Morales D, Cuppertino VM et al (2016) Letter to editor: Escherichia coli harbouring mcr-1 gene isolated from poultry not exposed to polymyxins in Brazil. Euro Surveill 21(26):1–2

    Article  Google Scholar 

  10. Rau RB, de Lima-Morales D, Wink PL, Ribeiro AR, Martins AF, Barth AL (2018) Emergence of mcr-1 producing Salmonella enterica serovar Typhimurium from retail meat: first detection in Brazil. Foodborne Pathog Dis 15(1):58–59

    Article  PubMed  Google Scholar 

  11. Dalmolin TV, Martins AF, Zavascki AP, de Lima-Morales D, Barth AL (2018) Acquisition of the mcr-1 gene by a high-risk clone of KPC-2-producing Klebsiella pneumoniae ST437/CC258, Brazil. Diagn Microbiol Infect Dis 90(2):132–133

    Article  CAS  PubMed  Google Scholar 

  12. Dalmolin TV, Castro L, Mayer FQ, Zavascki AP, Martins AF, Lima-Morales D, Barth AL (2017) Co-occurrence of mcr-1 and bla KPC-2 in a clinical isolate of Escherichia coli in Brazil. J Antimicrob Chemother 72(8):2404–2406

    Article  CAS  PubMed  Google Scholar 

  13. Monteiro J, Widen RH, Pignatari AC, Kubasek C, Silbert S (2012) Rapid detection of carbapenemase genes by multiplex real-time PCR. J Antimicrob Chemother 67:906–909

    Article  CAS  PubMed  Google Scholar 

  14. Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST) (2018) Tabelas de pontos de corte para interpretação de CIMs e diâmetros de halos. Versão 8:0

    Google Scholar 

  15. Bakthavatchalam YD, Veeraraghavan B, Mathur P, Purighalla S, Richard VS (2016) Polymyxin Nordmann/Poirel test for rapid detection of polymyxin resistance in Enterobacteriaceae: Indian experience. Indian J Med Microbiol 34:564–565

    Article  PubMed  Google Scholar 

  16. Poirel L, Larpin Y, Dobias J, Stephan R, Decousser JW, Madec JY, Nordmann P (2018) Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes. Diagn Microbiol Infect Dis 90(1):7–10

    Article  CAS  PubMed  Google Scholar 

  17. Simar S, Sibley D, Ashcraft D, Pankey G (2017) Evaluation of the rapid polymyxin NP test for polymyxin B resistance detection using Enterobacter cloacae and Enterobacter aerogenes isolates. J Clin Microbiol 55(10):3016–3020

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was supported by Fundo de Incentivo a Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE/HCPA) (project no. 16-0559). T.V. D. and H.A. were supported by a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico—Brazil (CNPq).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Afonso Luís Barth.

Additional information

Responsible Editor: Nilton Lincopan

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dalmolin, T.V., Dias, G.Á., de Castro, L.P. et al. Detection of Enterobacterales resistant to polymyxins using Rapid Polymyxins NP test. Braz J Microbiol 50, 425–428 (2019). https://doi.org/10.1007/s42770-019-00053-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42770-019-00053-x

Keywords

Navigation