Brazilian Journal of Microbiology

, Volume 50, Issue 1, pp 313–320 | Cite as

Gonadal pathogenicity of an infectious bronchitis virus strain from the Massachusetts genotype

  • Nicole Assis PereiraEmail author
  • Antônio Carlos Alessi
  • Hélio José Montassier
  • Ricardo José Garcia Pereira
  • Sueli Akemi Taniwaki
  • Viviane Fongaro Botosso
  • Bruno Rogério Rui
  • Leonardo José Richtzenhain
Veterinary Microbiology - Research Paper


An outbreak of infectious bronchitis caused by the IBVPR03 strain of the Massachusetts genotype affected H-120 vaccinated laying hens in South Brazil. We investigated the cross protection of the vaccine by assessing the traqueal ciliostasis, virus recovery, and histopathological changes typically observed in the respiratory tract. Although the IBVPR03 strain is S1-genotyped as Massachusetts with a high genomic similarity to the H-120 vaccine strains, surprisingly, we found no tropism or pathogenicity to the trachea in birds infected with this strain. On the other hand, we observed ovarian and testicle lesions. Here, we show that, despite belonging in the Massachusetts genotype, the IBVPR03 pathotype differs from the expected respiratory pattern, causing instead marked histopathological changes in the gonads, so far not associated with this group.


Infectious bronchitis virus Gonadal pathogenicity Young chicks Testis Ovaries 



We thank Adriana da Costa Neves (Laboratório de Genética, Instituto Butantan, São Paulo, Brazil) for pathology suggestions of the manuscript and Magna Aparecida Maltauro Soares by the assistance in histological sections.

Funding information

This research was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) under grant 04/11040-4.

Compliance with ethical standards

All animal procedures were approved by the Institutional Animal Care and Use Committee of Universidade de São Paulo (USP).


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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Nicole Assis Pereira
    • 1
    • 2
    Email author
  • Antônio Carlos Alessi
    • 3
  • Hélio José Montassier
    • 3
  • Ricardo José Garcia Pereira
    • 4
  • Sueli Akemi Taniwaki
    • 5
  • Viviane Fongaro Botosso
    • 2
  • Bruno Rogério Rui
    • 4
  • Leonardo José Richtzenhain
    • 5
  1. 1.Instituto de Ciências BiomédicasUniversidade de São PauloSão PauloBrazil
  2. 2.Laboratório de VirologiaInstituto ButantanSão PauloBrazil
  3. 3.Departamento de Patologia VeterináriaFaculdade de Ciências Agrárias e Veterinárias UNESP- JaboticabalJaboticabalBrazil
  4. 4.Departamento de Reprodução AnimalUniversidade de São PauloSão PauloBrazil
  5. 5.Departamento de Medicina Veterinária Preventiva, Faculdade de Medicina Veterinária e ZootecniaUniversidade de São PauloSão PauloBrazil

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