As one of the most common forms of skin injuries, skin burns are often accompanied by edema pain, suppuration of infection, slow tissue regeneration, and severe scar formation, which significantly delay wound healing as well as affect the quality of life. We prepared multifunctional electrospun poly(L-lactide-co-glycolide)/gelatin (P/G)-based dressings to synergistically harness the therapeutic benefits of peppermint essential oil (T), burn ointment (B), and Oregano essential oil (O) (P/G@TBO) for skin regeneration in punch and burn injury models. The P/G@TBO can afford the sustained release of bioactive cues for up to 72 h as well as remarkably promote cell migration (ca. P/G@TBO, 89% vs. control group, 51%) at 24 h. The P/G@TBO membranes also showed significant angiogenic effect as well as antibacterial and anti-inflammatory properties than that of the control group in vitro. Moreover, P/G@TBO dressings enabled fast wound healing (ca. P/G@TBO, 100% wound closure vs. control group, 95%) in a full-thickness excisional defect model up to 14 days in rats. Further evaluation of membranes in different animal models, including tail wagging model, facial itch model, and hot burn injury model showed significant pain relieve effect as well as itching and swelling relief functions during earlier stages of wound healing. Membranes were next transplanted into a scalded wound model in rats and an ear punch wound model in rabbits, which manifested antibacterial and anti-inflammatory properties and promoted re-epithelialization to achieve scarless wound healing percentage wound closure at day 28: P/G@TBO, 96% vs. control group 66%. Taken together our approach of simultaneously harnessing T, B, and O to enable multifunctionality to fibrous dressings may hold great promise for burn wound healing applications and other related disciplines.
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Human umbilical vein endothelial cells
Reactive oxygen species
Peppermint essential oil
- E. coli:
Oregano essential oil
- S. aureus:
Fetal bovine serum
Dulbecco's Modified Eagle Medium
Rotations per minute
- V c :
Scanning electron microscopy
Red blood cells
Water contact angle
Masson’s trichrome staining
Hematoxylin and Eosin
Fibroblast growth factor
Drug delivery system
- T max :
Maximum degradation temperature
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This work was supported by Science and Technology Commission of Shanghai Municipality (No. 20S31900900, 20DZ2254900), Sino German Science Foundation Research Exchange Center (M-0263), Taishan Scholars Program of Shandong Province (tsqn201812141), Shandong Provincial Natural Science Foundation (ZR2021MH004) and Academic promotion program of Shandong First Medical University (2019RC016). This project was also supported by Researchers Supporting Project Number (RSP2023R65), King Saud University, Riyadh, Saudi Arabia, China Education Association for International Exchange (2022181) and Donghua University Postgraduate Innovation and Entrepreneurship Ability Training Program (yjssc2023002). This research was also partially supported by Grant-in-Aid for JSPS Research Fellows (JP21F21353). M.S. is an International Research Fellow of the Japan Society for the Promotion of Science (Postdoctoral Fellowships for Research in Japan (Standard)). M.S. is keenly grateful to the Gifu University, Japan for Special Cooperative Research Program and College of Biological Science and Medical Engineering, Donghua University, Shanghai, China for cooperative research.
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Yuan, Z., Zhao, Y., Shafiq, M. et al. Multi-Functional Fibrous Dressings for Burn Injury Treatment with Pain and Swelling Relief and Scarless Wound Healing. Adv. Fiber Mater. 5, 1963–1985 (2023). https://doi.org/10.1007/s42765-023-00320-5