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Response of Soil Dehydrogenase Activity to Salinity and Cadmium Species

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Abstract

Greater understanding of the microbial activity role in metal mobilization processes in soil is of major importance. The objective was to study the effect of major Cd species in solution of a saline soil on dehydrogenase activity (DHA). Hypothesis is that (i) under increased soil salinity, more mobile Cd species with diverse effect on DHA may be generated (i.e., CdCln2-n); and that (ii) DHA may correlate to organically complexed Cd species. In a greenhouse pot experiment, NaCl salinity (50 and 100 mM) was applied to control soil and soil spiked with Cd (5 and 10 mg kg-1). Soil total and available (CaCl2 extractable) Cd concentrations were measured, and DHA determined using 2,3,5-triphenyltetrazolium chloride (TTC) method. Speciation was calculated from results of soil solution ion analysis using geochemical equilibrium model Visual MINTEQ. DHA was reduced with increased soil salinity and Cd contamination, but only compared with control soil. Although weak, negative correlation between DHA and CdCln2-n complexes in soil solution suggested their higher inhibitory effect on DHA than other Cd species. Positive correlation between DHA and organically complexed Cd indicated that raised microbial activity may increase the proportion of organically complexed Cd in the soil solution. Cd toxicity to soil microorganisms can be accentuated in a saline environment, which may be an issue of great importance for agricultural production in coastal areas. Microbial activity may via releasing organic substances in soil solution significantly change cadmium complexation and mobility in soil, an aspect which has often been overlooked.

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References

  • Aydin M, Tombuloglu G, Sakcali MS, Hakeem KR, Tombuloglu H (2019) Boron alleviates drought stress by enhancing gene expression and antioxidant enzyme activity. J Soil Sci Plant Nutr 19(3):545–555

    Article  CAS  Google Scholar 

  • Casida L, Klein D, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376

    Article  CAS  Google Scholar 

  • Das SK, Varma A (2011) Role of enzymes in maintaining soil health. In: Shukla G, Varma A (eds) Soil enzymology. Springer-Verlag, Berlin Heidelberg, pp 25–42

    Google Scholar 

  • Dussault M, Bécaert V, François M, Sauvé S, Deschęnes L (2008) Effect of copper on soil functional stability measured by relative soil stability index (RSSI) based on two enzyme activities. Chemosphere 72:755–762

    Article  CAS  Google Scholar 

  • Filipović L, Romić M, Romić D, Filipović V, Ondrašek G (2018) Organic matter and salinity modify cadmium soil (phyto) availability. Ecotoxicol Environ Saf 147:824–831

    Article  Google Scholar 

  • Gartley KL (2011) Recommended methods for measuring soluble salts in soils. In: Recommended Soil Testing Procedures for the Northeastern United States. The Northeast Coordinating Committee for Soil Testing (NECC-1312), Northeastern Regional Publication No. 493, 3rd Edition 493:87–94 Available at (Chapter 10): https://www.udel.edu/academics/colleges/canr/cooperative-extension/fact-sheets/soil-testing-procedures-northeastern-US/

  • Gianfreda L (2015) Enzymes of importance to rhizosphere processes. J Soil Sci Plant Nutr 15(2):283–306

    CAS  Google Scholar 

  • Gustafsson JP (2012) Visual MINTEQ Version 3.0., Stockholm, Sweden

  • Haynes RJ (2005) Labile organic matter fractions as central components of the quality of agricultural soils: an overview. Adv Agron 85:221–268

    Article  CAS  Google Scholar 

  • Hirzel J, Retamal-Salgado J, Walter I, Matus I (2019) Residual effect of cadmium applications in different crop rotations and environments on durum wheat cadmium accumulation. J Soil Water Conserv 74(1):41–50

    Article  Google Scholar 

  • Houba VJG, Uittenbogaard J, Pellen P (1996) Wageningen evaluating programs for analytical laboratories (WEPAL), organization and purpose. Commun Soil Sci Plant Anal 27:421–431

    Article  CAS  Google Scholar 

  • Houba VJG, Temminghoff EJM, Gaikhorst GA, van Vark W (1999) Soil analysis procedures using 0.01 M calcium chloride as extraction reagent. Commun Soil Sci Plant Anal 31:1299–1396

    Article  Google Scholar 

  • Howard PJA (1972) Problems in the estimation of biological activity in soil. Oikos 23:235–240

    Article  CAS  Google Scholar 

  • ISO 11466:1995 (HRN ISO 11466:2004) (2004) Soil quality-extraction of trace elements soluble in aqua regia. International standard, Croatian Standards Institute, Zagreb

  • ISO 23753-1:2005 (2005) Soil quality-determination of dehydrogenase activity in soils - part 1: method using triphenyltetrazolium chloride (TTC). International standard, Croatian Standards Institute, Zagreb

    Google Scholar 

  • Kinniburgh DG, Milne CJ, Benedetti MF, Pinheiro JP, Filius J, Koopal LK (1996) Metal ion binding by humic acid: application of the NICA-Donnan model. Environ Sci Technol 30:1687–1698

    Article  CAS  Google Scholar 

  • Kizilkaya R, Askin T, Bayrakli B, Saglam M (2004) Microbiological characteristics of soils contaminated with heavy metals. Eur J Soil Biol 40:95–102

    Article  CAS  Google Scholar 

  • Machuca A, Córdova C, Stolpe NB, Barrera JA, Chávez D, Almendras K, Bonilla AM (2018) In vitro sensitivity of forest soil enzymes to temperature increase in Western Patagonia. J Soil Sci Plant Nutr 18(1):202–219

    CAS  Google Scholar 

  • Masto R, Ahirwar R, George J, Ram L, Selvi V (2011) Soil biological and biochemical responses to Cd exposure. Open J Soil Sci 1(1):8–15

    Article  CAS  Google Scholar 

  • Matijević L, Romić D, Romić M (2014) Soil organic matter and salinity affect copper bioavailability in root zone and uptake by Vicia faba L. plants. Environ Geochem Health 36(5):883–896

    Article  Google Scholar 

  • Ondrašek G, Romić D, Rengel Z, Romić M, Zovko M (2009) Cadmium accumulation by muskmelon under salt stress in contaminated organic soil. Sci Total Environ 407:2175–2182

    Article  Google Scholar 

  • Pan J, Yu L (2011) Effects of Cd or/and Pb on soil enzyme activities and microbial community structure. Ecol Eng 37:1889–1894

    Article  Google Scholar 

  • Rath KM, Maheshwari A, Bengtson P, Rousk J (2016) Comparative toxicities of salts on microbial processes in soil. Appl Environ Microbiol 82:2012–2020

    Article  CAS  Google Scholar 

  • Romić D, Romić M, Zovko M, Bakić H, Ondrašek G (2012) Trace metals in the coastal soils developed from estuarine floodplain sediments in the Croatian Mediterranean region. Environ Geochem Health 34:399–416

    Article  Google Scholar 

  • SAS Institute Inc. (2001) SAS/STAT User's guide, version 8-1. Cary North Carolina: SAS Institute Inc

  • Schützendübel A, Polle A (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1356

    PubMed  Google Scholar 

  • Seshadri B, Bolan NS, Naidu R (2015) Rhizosphere-induced heavy metal(loid) transformation in relation to bioavailability and remediation. J Soil Sci Plant Nutr 15(2):524–548

    CAS  Google Scholar 

  • Singh P, Choudhary OP, Mavi MS (2018) Irrigation-induced salinization effects on soil chemical and biological properties under cotton-wheat rotation on loamy sand soil in northwest India. J Indian Soc Soil Sci 66(4):386–391

    Article  Google Scholar 

  • Tian H, Kong L, Megharaj M, He W (2017) Contribution of attendant anions on cadmium toxicity to soil enzymes. Chemosphere 187:19–26

    Article  CAS  Google Scholar 

  • Tripathi S, Chakraborty A, Chakrabarti K, Bandyopadhyay BK (2007) Enzyme activities and microbial biomass in coastal soils of India. Soil Biol Biochem 39(11):2840–2848

    Article  CAS  Google Scholar 

  • Wolińska A, Stępniewska Z (2012) Dehydrogenase activity in the soil environment, In: Canuto RA (ed.) Dehydrogenases. InTech Rijeka, pp 183–210. https://doi.org/10.5772/48294

  • Wyszkowska J, Wyszkowski M (2003) Effect of cadmium and magnesium on enzymatic activity in soil. Pol J Environ Stud 12:473–479

    CAS  Google Scholar 

  • Xie W, Zhou J, Wang H, Chen X, Lu Z, Yu J, Chen X (2009) Short-term effects of copper, cadmium and cypermethrin on dehydrogenase activity and microbial functional diversity in soils after long-term mineral or organic fertilization. Agric Ecosyst Environ 129:450–456

    Article  CAS  Google Scholar 

  • Yan N, Marschner P, Cao W, Zuo C, Qin W (2015) Influence of salinity and water content on soil microorganisms. Int Soil Water Conserv Res 3:316–323

    Article  Google Scholar 

  • Yang F, Tian J, Fang H, Gao Y, Xu M, Lou Y, Zhou B, Kuzyakov Y (2019) Functional soil organic matter fractions, microbial community, and enzyme activities in a Mollisol under 35 years manure and mineral fertilization. J Soil Sci Plant Nutr 19(2):430–439

    Article  CAS  Google Scholar 

  • Yuan B-C, Li Z-Z, Liu H, Gao M, Zhang Y-Y (2007) Microbial biomass and activity in salt affected soils under arid conditions. Appl Soil Ecol 35:319–328

    Article  Google Scholar 

Download references

Funding

This research was supported by the Ministry of Science, Education and Sport of the Republic of Croatia, contract 178-1782221-0350; project “Soil salinization: identifying, processes and effects on crops”.

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Authors and Affiliations

  1. Department of Soil Amelioration, University of Zagreb Faculty of Agriculture, Svetosimunska 25, 10000, Zagreb, Croatia

    Lana Filipović, Marija Romić, Vilim Filipović & Davor Romić

  2. Department of Microbiology, University of Zagreb Faculty of Agriculture, Svetosimunska 25, 10000, Zagreb, Croatia

    Sanja Sikora

  3. Genera, Dechra Pharmaceuticals PLC, Svetonedeljska 2, 10436 Rakov Potok, Zagreb, Croatia

    Katarina Huić Babić

  4. Research Area 1 “Landscape Functioning”, Working Group “Hydropedology”, Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalder Straße 84, 15374, Müncheberg, Germany

    Horst H. Gerke

Authors
  1. Lana Filipović
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  2. Marija Romić
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  3. Sanja Sikora
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  4. Katarina Huić Babić
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  5. Vilim Filipović
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  6. Horst H. Gerke
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  7. Davor Romić
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Correspondence to Lana Filipović.

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Filipović, L., Romić, M., Sikora, S. et al. Response of Soil Dehydrogenase Activity to Salinity and Cadmium Species. J Soil Sci Plant Nutr 20, 530–536 (2020). https://doi.org/10.1007/s42729-019-00140-w

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  • DOI: https://doi.org/10.1007/s42729-019-00140-w

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