Zn-DTPA-HEDTA-EDTA Application: a Strategy to Improve the Yield and Plant Quality of a Barley Crop While Reducing the N Application Rate

Abstract

Over-use of N fertilization has been common in order to obtain the highest possible grain yield. We investigated the efficiency of combining the application of N and ZnCHE (Zn-DTPA-HEDTA-EDTA). Different rates (30, 60, or 90 kg ha−1) and sources [pig slurry(PS) or urea] of N and rates of ZnCHE (0, 0.5, 1, or 1.5 kg ha−1) were applied to a barley crop. Nitrogen fertilization combined with soil Zn applications had a significant interaction on various plant parameters (grain protein concentration, yield, Zn uptake, and N uptake). An application rate of 90 kg N ha−1 seems recommendable to obtain high values for both crop yield and N uptake by the plant. PS application was associated with higher mean grain yield and Zn utilization than urea application, but with lower grain protein concentration. On the other hand, the lowest Zn application rate was sufficient to achieve a high grain yield (> 3200 kg ha−1). Higher Zn rates provided great Zn concentrations in the different parts of the plant. Furthermore, high grain protein concentrations (> 9.6%) were obtained with combinations of N60 or N90 and ZnCHE-1 or ZnCHE-1.5, both for PS and for urea. The application of this synthetic Zn chelate could be recommended as a strategy for reducing the N application rate but still obtaining high grain yield and nutritional value in barley. These effects may have been due not only to Zn application but also to the influence of chelating agents such as DTPA, HEDTA, and EDTA.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Abalos D, Jeffery S, Sanz-Cobena A, Guardia G, Vallejo A (2014) Meta-analysis of the effect of urease and nitrification inhibitors on crop productivity and nitrogen use efficiency. Agric Ecosyst Environ 189:136–144. https://doi.org/10.1016/j.agee.2014.03.036

    CAS  Article  Google Scholar 

  2. Abunyewa AA, Ferguson RB, Wortmann CS, Mason SC (2017) Grain sorghum nitrogen use as affected by planting practice and nitrogen rate. J Soil Sci Plant Nutr 17:155–166

    CAS  Google Scholar 

  3. Almendros P, Obrador A, Gonzalez D, Alvarez JM (2015) Biofortification of zinc in onions (Allium cepa L.) and soil Zn status bythe application of different organic Zn complexes. Sci Hortic 186:254–265. https://doi.org/10.1016/j.scienta.2015.02.023

    CAS  Article  Google Scholar 

  4. Arora S, Singh M (2004) Interaction effect of zinc and nitrogen on growth and yield of barley (Hordeum vulgare L.) on typic Ustipsamments. Asian J Plant Sci 3:101–103. https://doi.org/10.3923/ajps.2004.101.103

    Article  Google Scholar 

  5. Bower CA, Reitemeier RF, Fireman M (1952) Exchangeable cation analysis of saline and alkali soils. Soil Sci 73:251–262

    CAS  Article  Google Scholar 

  6. Cakmak I, Kutman UB (2018) Agronomic biofortification of cereals with zinc: a review. Eur J Soil Sci 69:172–180. https://doi.org/10.1111/ejss.12437

    Article  Google Scholar 

  7. Cakmak I, Pfeiffer WH, McClafferty B (2010) Biofortification of durum wheat with zinc and iron. Cereal Chem 87:10–20. https://doi.org/10.1094/CCHEM-87-1-0010

    CAS  Article  Google Scholar 

  8. Chaudhry FM, Kausar MA, Rashid A (1977) Mechanism of nitrogen effect on zinc nutrition of flooded rice. Plant Soil 46:649–654

    CAS  Article  Google Scholar 

  9. Council Directive 91/676/EEC (n.d.) of 12 December 1991 concerning the protection of waters against pollution caused by nitrates from agricultural sources. https://eur-lex.europa.eu/eli/dir/1991/676/oj. Accessed 1 Apr 2019

  10. Day PR (1965) Particle fractionation and particle-size analysis. American Society of Agronomy, Soil Science Society of America

  11. DOCM (2011) Orden 07/02/2011. In Spanish. http://www.castillalamancha.es/gobierno/agrimedambydesrur/estructura/dgaag/actuaciones/programa-de-actuaci%C3%B3n-en-zonas-vulnerables-la-contaminaci%C3%B3n-por-nitratos. Accessed 1 Apr 2019

  12. Erenoglu EB, Kutman UB, Ceylan Y, Yildiz B, Cakmak I (2011) Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat. New Phytol 189:438–448. https://doi.org/10.1111/j.1469-8137.2010.03488.x

    CAS  Article  PubMed  Google Scholar 

  13. Eurostat (2018) Pig population – anual data. http://ec.europa.eu/eurostat/data/database?node_code=apro_mt_lspig. Accessed 1 Apr 2019

  14. FAOstat (2018) Food and agriculture data. http://www.fao.org/faostat/en/#data/QD. Accessed 1 Apr 2019

  15. FEDNA (2016) Fundación Española para el Desarrollo de la Nutrición Animal. In Spanish. http://www.fundacionfedna.org/node/495. Accessed 1 Apr 2019

  16. Gonzalez D, Almendros P, Obrador A, Alvarez JM (2019) Zinc application in conjunction with urea as a fertilization strategy for improving both nitrogen use efficiency and the zinc biofortification of barley. J Sci Food Agric 99:4445–4451

    CAS  Article  Google Scholar 

  17. Guo JX, Feng ZM, Hu XY, Tian GL, Ling N, Wang JH, Shen QR, Guo SW (2016) Affects of soil zinc availability, nitrogen fertilizer rate and zinc fertilizer application method on zinc biofortification of rice. J Agric Sci 154:584–597. https://doi.org/10.1017/S0021859615000441

    CAS  Article  Google Scholar 

  18. Guo XL, Chen L, Zheng RB, Zhang K, Qiu YP, Yue HT (2019) Differences in soil nitrogen availability and transformation in relation to land use in the Napahai wetland. Southwest China. J Soil Sci Plant Nutr 19:92–97

    Article  Google Scholar 

  19. Harapiak J, Karamanos R, Johnston A (2000) High yielding barley production (Canadian prairies). Better Crops 84. http://www.ipni.net/publication/bettercrops.nsf/issue/BC-2000-1. Accessed 1 Apr 2019

  20. Hu Z, Chandran K, Grasso D, Smets BF (2003) Nitrification inhibition by Ethylenediamine-based chelating agents. Environ Eng Sci 20. https://www.liebertpub.com/doi/abs/10.1089/109287503321671429. Accessed 1 Apr 2019:219–228

    CAS  Article  Google Scholar 

  21. Intawongse M, Dean JR (2006) Uptake of heavy metals by vegetable plants grown on contaminated soil and their bioavailability in the human gastrointestinal tract. Food Addit Contam 23:36–48. https://doi.org/10.1080/02652030500387554

    CAS  Article  PubMed  Google Scholar 

  22. Kabata-Pendias A (2001) Trace Elements in Soils and Plants. CRC Press, Boca Raton

  23. Kapoor V, Li X, Elk M, Chandran K, Impellitteri CA, Santo-Domingo JW (2015) Impact of heavy metals on transcriptional and physiological activity of nitrifying bacteria. Environ Sci Technol 49:13454–13462. https://doi.org/10.1021/acs.est.5b02748

    CAS  Article  PubMed  Google Scholar 

  24. Kutman UB, Yildiz B, Ozturk L, Cakmak I (2010) Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen. Cereal Chem 87:1–9. https://doi.org/10.1094/CCHEM-87-1-0001

    CAS  Article  Google Scholar 

  25. Kutman UB, Yildiz B, Cakmak I (2011) Effect of nitrogen on uptake, remobilization, and partitioning of zinc and iron throughout the development of durum wheat. Plant Soil 342:149–164. https://doi.org/10.1007/s11104-010-0679-5

    CAS  Article  Google Scholar 

  26. Ladan S, Jacinthe PA (2017) Nitrogen availability and early corn growth on plowed and no-till soils amended with different types of cover crops. J Soil Sci Plant Nutr 17:74–90

    CAS  Google Scholar 

  27. Leleyter L, Probst JL, Depetris P, Haida S, Mortatti J, Rouault R, Samuel J (1999) REE distribution pattern in river sediments: partitioning into residual and labile fractions. C R Acad Sci Series IIA Paris 329:45–52

    CAS  Google Scholar 

  28. Lindsay WL (1979) Chelate equilibria. In: John Wiley and Sons (ed) Chemical Equilibria in Soils. Wiley, New York, pp 238–263

    Google Scholar 

  29. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Am J 42:421–428. https://doi.org/10.2136/sssaj1978.03615995004200030009x

    CAS  Article  Google Scholar 

  30. Liu DY, Zhang W, Pang LL, Zhang YQ, Wang XZ, Liu YM, Chen XP, Zhang FS, Zou CQ (2017) Effects of zinc application rate and zinc distribution relative to root distribution on grain yield and grain Zn concentration in wheat. Plant Soil 411:167–178

    CAS  Article  Google Scholar 

  31. Lopez-Bellido (2009) Abonado de los cereales de invierno: trigo y cebada. In: Guía práctica de la fertilización racional de los cultivos en España, parte II. Ministerio de Medio Ambiente y Medio Rural y Marino. In Spanish. https://www.mapama.gob.es/es/agricultura/publicaciones/Publicaciones-fertilizantes.aspx. Accessed 1 Apr 2019

  32. MAPA (1994) Métodos oficiales de análisis, vol III. Ministerio de Agricultura, Pesca y Alimentación, Madrid

    Google Scholar 

  33. Montoya M, Castellano-Hinojosa A, Vallejo A, Alvarez JM, Bedmar EJ, Recio J, Guardia G (2018) Zinc fertilizers influence greenhouse gas emissions and nitrifying and denitrifying communities in a non-irrigated arable cropland. Geoderma. 325:208–217. https://doi.org/10.1016/j.geoderma.2018.03.035

    CAS  Article  Google Scholar 

  34. Mortvedt JJ, Gilkes RJ (1993) Zinc fertilizers. In: Robson AD (ed) Zinc in soils and plants, developments in plant and soil science 55. Kluwer Academic, Dordrecht, pp 33–34

    Google Scholar 

  35. Olsen SR (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Dep Agric Circ 939:1–19

    Google Scholar 

  36. Pagani A, Sawyer JE, Mallarino A (2013) Site-specific nutrient management: for nutrient management planning to improve crop production, environmental quality, and economic return. Extension and Outreach Publications 116. https://lib.dr.iastate.edu/extension_pubs/116. Accessed 1 Apr 2019

  37. Perchlik M, Tegeder M (2017) Improving plant nitrogen use efficiency through alteration of amino acid transport processes. Plant Physiol 175:235–247. https://doi.org/10.1104/pp.17.00608

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  38. Pinochet D, Clunes J, Gauna C, Contreras A (2018) Reasoned fertilization of potato in response to nitrogen supply in Andisols. J Soil Sci Plant Nutr 18:790–803

    Google Scholar 

  39. Podlesakova E, Nemecek J, Vácha R (2001) Mobility and bioavailability of trace elements in soils. In: Iskandar IK, Kickham MB (eds) Trace elements in soil. Bioavailability, flux and transfer. Lewis Publishers, Boca Raton, pp 21–42

    Google Scholar 

  40. Prasad B, Sinha MK (1981) The relative efficiency of zinc carriers on growth and zinc nutrition of corn. Plant Soil 62:45–52 https://link.springer.com/article/10.1007/BF02205024. Accessed 1 Apr 2019

    CAS  Article  Google Scholar 

  41. Real Decreto 261/1996 In Spanish (n.d.). https://www.boe.es/buscar/doc.php?id=BOE-A-1996-5618. Accessed 1 Apr 2019

  42. Sajad A, Jamil M, Ahmad M (2014) An investigation of nitrogen-zinc interaction synergise maize (Zea mays L.) fooder quality. WASJ. 31:91–95 https://www.idosi.org/wasj/wasj31(1)14/12.pdf. Accessed 1 Apr 2019

    Google Scholar 

  43. Sanchez M, Gonzalez JL (2005) The fertilizer value of pig slurry. I Values depending on the type of operation. Bioresour Technol 96:1117–1123. https://doi.org/10.1016/j.biortech.2004.10.002

    CAS  Article  PubMed  Google Scholar 

  44. Singh MV (2008) Micronutrient deficiencies in crops and soils in India. In: Alloway BJ (ed) Micronutrient deficiencies in global crop production. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6860-7_4

    Google Scholar 

  45. Singh M, Singh SP (1981) Effect of nitrogen and zinc opn the yield of submerged rice and uptake of N and Zn on unlimed and limed soils. Plant Soil 62:183–192. https://doi.org/10.15835/nbha4229469

    CAS  Article  Google Scholar 

  46. Smith RM, Martell AE, Motekaitis RJ (2004) NIST critically selected stability constants of metal complexes. In: Standard reference data program. National Institute of Standards and Technology, Gaithersburg

    Google Scholar 

  47. Soil Survey Staff (2010) In: USDA (ed) Keys to soil taxonomy, 11th edn. Natural Resources Conservation Service, Washington, DC

    Google Scholar 

  48. Spiertz JHJ (2010) Nitrogen, sustainable agriculture and food security. A review. Agron Sustain Dev 30:43–55

    CAS  Article  Google Scholar 

  49. Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S (2002) Agricultural sustainability and intensive production practices. Nature 418:671–677. https://doi.org/10.1038/nature01014

    CAS  Article  PubMed  Google Scholar 

  50. Tran TS, Simard RR (1993) Mehlich III-extractable elements. In: Carter MR (ed) Soil sampling and methods of analysis, 1st edn. Can. Soc. Soil Sci. Lewis Publishers, Boca Ratón, pp 43–49

    Google Scholar 

  51. Velu G, Ortiz-Monasterio J, Cakmak I, Hao Y, Singh RP (2014) Biofortification strategies to increase grain zinc and iron concentrations in wheat. J Cereal Sci 59:365–372. https://doi.org/10.1016/j.jcs.2013.09.001

    CAS  Article  Google Scholar 

  52. Verma SS, Singh N, Joshi YP, Deodari V (2005) Effect of N and Zn on growth characters, herbage yield, nutrient uptake and quality of fodder for sorghum. Indian J Agron 50:167–169 http://www.indianjournals.com/ijor.aspx?target=ijor:ija&volume=50&issue=2&article=026. Accessed 1 Apr 2019

    CAS  Google Scholar 

  53. Zeidan MS, Mohamed MF, Hamouda HA (2010) Effect of foliar fertilization of Fe, Mn and Zn on wheat yield and quality in low sandy soils fertility. WJAS. 6:696–699 http://idosi.org/wjas/wjas6(6).htm. Accessed 1 Apr 2019

    CAS  Google Scholar 

  54. Zhang H, Yu X, Jin Z, Zheng W, Zhai B, Li Z (2017) Improving grain yield and water use efficiency of winter wheat through a combination of manure and chemical nitrogen fertilizer on the loess plateau, China. J Soil Sci Plant Nutr 19:92–97

    Google Scholar 

Download references

Funding

This work was funded by the Comunidad de Madrid (Spain) and Structural Funds 2014-2020 (ERDF and ESF) (projects AGRISOST-CM S2013/ABI-2717 and S2018/BAA-4330).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Patricia Almendros.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Almendros, P., Obrador, A., Alvarez, J.M. et al. Zn-DTPA-HEDTA-EDTA Application: a Strategy to Improve the Yield and Plant Quality of a Barley Crop While Reducing the N Application Rate. J Soil Sci Plant Nutr 19, 920–934 (2019). https://doi.org/10.1007/s42729-019-00090-3

Download citation

Keywords

  • N-Zn combined fertilization
  • Yield
  • Nutritional composition
  • Protein
  • Agronomic efficiency
  • Utilization efficiency