The Burning of Sugarcane Plantation in the Tropics Modifies the Microbial and Enzymatic Processes in Soil and Rhizosphere

  • A. Trujillo-Narcía
  • M. C. Rivera-CruzEmail author
  • M. Magaña-Aquino
  • E. A. Trujillo-Rivera
Original Paper


In this study were examined chemical, microbiological, and enzymatic changes at different depths of the soil and rhizosphere, produced by the burning of a commercial sugarcane crop in tropical areas of México. Samples of silty loamy soil and rhizosphere were collected at three times in the sugarcane production cycle: before burning (BB), after first burning (AFB), and after second burnings (ASB), with a general interval of 15 days between the first and the third collection date. Soil organic matter (SOM), soil organic carbon (SOC), total nitrogen (Nt), phosphorus available (Pav), pH, and the C/N ratio were determined in soil and rhizosphere, as well as the enzymatic activities of phosphatase and urease. Furthermore, microbial respiration, microbial biomass, and nitrogen-fixing bacteria (NFB) and phosphate solubilizing bacteria (PSB) densities were monitored during 84 days. The Pav and the pH increased significantly in soil samples affected by the second burning of the stubbles, but SOM, SOC, Nt, the C/N ratio, phosphatase, and urease activities decreased as a result of the first and second burnings. This decrease was more pronounced in non-rhizospheric soil. The densities of NFB and PSB increased with the burning, as well as microbial respiration. All the variables evaluated recorded higher values in the soil surface layer.


Bacteria Nitrogen Phosphorus Phosphatase Urease Respiration 


Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. Adeniji BAG, Ige O, Oluwole O, Olopade C (2013) Exposure to emissions from firewood cooking stove and the pulmonary health of women in Olorunda community, Ibadan, Nigeria. Air Qual Atmos Health 6:465–471. CrossRefGoogle Scholar
  2. Ajwa HA, Dell CJ, Rice CW (1999) Changes in enzyme activities and microbial biomass of tallgrass prairie soil as related to burning and nitrogen fertilization. Soil Biol Biochem 31:769–777. CrossRefGoogle Scholar
  3. Alves FD, da Silva RTV (2014) Use of sugarcane straw ash for zeolite synthesis. Int J Energy Environ 52:559–566Google Scholar
  4. Amu OO, Ogunniyi SA, Oladeji OO (2011) Geotechnical properties of lateritic soil stabilized with sugarcane straw ash. Am J Sci Ind Res 2:323–331. Google Scholar
  5. Anderson TH, Domsch KH (2010) Soil microbial biomass: the eco-physiological approach. Soil Biol Biochem 42:2039–2043. CrossRefGoogle Scholar
  6. Arias-Trinidad A, Rivera-Cruz MC, Roldán-Garrigós A, Aceves-Navarro LA, Quintero-Lizaola R, Hernández-Guzmán J (2017) Uso de Leersia hexandra (Poaceae) en la fitorremediación de suelos contaminados con petróleo fresco e intemperizado. Int J Trop Biol 65:21–30. Google Scholar
  7. Balestrini R, Lumini E, Borriello R, Bianciotto V (2015) Plant-soil biota interactions. In: Paul EA (ed) Soil microbiology, ecology, and biochemistry. Elsevier, Colorado State University, pp 311–338CrossRefGoogle Scholar
  8. Bárcenas-Moreno G, García-Orenes F, Mataix-Solero J, Mataix-Beneyto J, Bääth E (2011) Soil microbial recolonisation after a fire in a Mediterranean forest. Biol Fert Soil 47:261–272. CrossRefGoogle Scholar
  9. Belen TM, Bueis TM, La Fuente L, Lopez O, San Jose E, Eleftheriadis A, Mulas R (2012) Effects on soil phosphorus dynamics of municipal solid waste compost addition to a burnt and unburnt forest soil. Sci Total Environ 642:374–384. Google Scholar
  10. Beri V, Goswami KP, Brar SA (1978) Urease activity and its Michhaelis constant for soil systems. Plant Soil 49:105–115. CrossRefGoogle Scholar
  11. Bielefeld NG, da Cunha BMM (2003) Effects of fire on soil nitrogen dynamics and microbial biomass in savannas of Central Brazil. Pesq Agropec Bras 38:955–962. CrossRefGoogle Scholar
  12. Brady CN, Weil RR (2008) The nature and properties of soils, 14th edition revised. Pearson Prentice Hall, New JerseyGoogle Scholar
  13. Bremmer JM, Mulvarey CS (1982) Nitrogen total. In: Page AL, Miller RH, Keeny DR (eds) Methods of soil analysis. Part 2 chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, pp 595–622Google Scholar
  14. Bronick J, Lal R (2005) Soil structure and management: a review. Geoderma 124:3–22. CrossRefGoogle Scholar
  15. Cerón RL, Melharejo MLM (2005) Enzimas del suelo: Indicadores de salud y calidad. Acta Biol Colom 10:5–18. Google Scholar
  16. Chen CR, Condron LM, Davis MR, Sherlock RR (2002) Phosphorus dynamics in the rhizosphere of perennial ryegrass (Lolium perenne L.) and radiata pine (Pinus radiata D. Don.). Soil Biol Biochem 34:487–499. CrossRefGoogle Scholar
  17. Díaz-Fierros VF, Benito E, Vega JA, Castelao A, Soto B, Pérez R, Taboada T (1990) Solute loss and soil erosion in burnt soil from Galicia (NW Spain). In: Fire and ecosystems dynamics: Mediterranean and northern perspective. SPB Academic Publishing, pp 103-116Google Scholar
  18. Docherty KM, Balser CT, Bohannan JMB, Gutknecht MLJ (2012) Soil microbial responses to fire and interacting global change factors in a California annual grassland. Biogeochemistry. 109:63–83. CrossRefGoogle Scholar
  19. Duiker SW, Lal R (1999) Crop residue and tillage effects on carbon sequestration in a Luvisol in Central Ohio. Soil Tillage Res 52:73–81. CrossRefGoogle Scholar
  20. Egamberbieva D (2009) Alleviation of Sal stress by plant growth regulators and IAA produccing bacteria in wheat. Acta Physiol Plant 31:861–864. CrossRefGoogle Scholar
  21. FAO Statistical Pocketbook (2015) World Food and Agriculture. Accessed 5 february 2018
  22. Franzluebbers AJ, Wright SF, Stuedemann JA (2000) Soil aggregation and glomalin under pastures in the southern Piedmont USA. Soil Sci Soc Am J 64:1018–1026. CrossRefGoogle Scholar
  23. Galdos MV, Cerri CC, Cerri CEP (2009) Soil carbon stocks under burned and unburned sugarcane in Brazil. Geoderma 153:347–352. CrossRefGoogle Scholar
  24. Goberna M, Garcia C, Insam H, Hernández MT, Verdu M (2012) Burning fire-prone Mediterranean shrublands: immediate changes in soil microbiol community structure and ecosystem functions. Microb Ecol 64:242–255. CrossRefGoogle Scholar
  25. Graham MH, Haynes RJ (2006) Organic matter status and the size, activity and metabolic diversity of the soil microbial community in the row and inter-row of sugarcane under burning and trash retention. Soil Biol Biochem 38:21–31. CrossRefGoogle Scholar
  26. Greenland JD, Hayes BHM (1981) Soil processes. In: Greenland JD, Hayes BHM (eds) The chemistry of soil processes. John Wiley & Sons Ltd, New York, pp 1–36Google Scholar
  27. Hu T, Sun L, Hu H, Guo F (2017) Effects of fire disturbance on soil respiration in the non-growing season in a Larix gmelinii forest in the Daxing’an mountains, China. PLoS One 12:e0180214. CrossRefGoogle Scholar
  28. INEGI (2017) Anuario estadístico y geográfico de Tabasco. Gobierno del estado de Tabasco, Villahermosa, TabascoGoogle Scholar
  29. INFOCANA (2017) Cierre de Zafra 2015–2016. Accessed 29 august 2018
  30. Jenkinson DC (1976) The effects of biocidal treatments on metabolism in soil. IV. The decomposition of fumigated organisms in soil. Soil Biol Biochem 8:203–208. CrossRefGoogle Scholar
  31. Jones JB, Wolf B, Mills HA (1992) Plant analysis handbook. In: A practical sampling, preparation, analysis, and interpretation guide. Micro-Macro Publishing, Inc., AthensGoogle Scholar
  32. Koga N, Hayashib K, Shimoda S (2016) Differences in CO2 and N2O emission rates following crop residue incorporation with or without field burning: a case study of adzuki bean residue and wheat straw. Soil Sci Plant Nutr 62:52–56. CrossRefGoogle Scholar
  33. Machado PEF, Lima E, Bacis CM, Urquiaga S, Alves RJB, Boddey MR (2010) Impact of pre-harvest burning versus trash conservation on soil carbon and nitrogen stocks on a sugarcane plantation in the Brazilian Atlantic forest region. Plant Soil 333:71–80. CrossRefGoogle Scholar
  34. Madigan MT, Martinko JM, Dunlap PV, Clark D (2015) Brock. Biología de los Microorganismos, 12a. ed. Pearson Educación, S.A. MadridGoogle Scholar
  35. Mertens K, Tolossa RA, Verdoodt A, Dumon M, Deckers J, Van Ranst E (2015) Impact of traditional soil burning (guie) on Planosol properties and land-use intensification in south-western Ethiopia. Soil Use Manag 31:330–336. CrossRefGoogle Scholar
  36. Mandal KU, Singh G, Victor SU, Sharma KL (2003) Green manuring: its effect on soil properties and crop growth under rice–wheat cropping system. Eur J Agron 19:225–237. CrossRefGoogle Scholar
  37. Marcote I, Hernández T, García C, Polo A (2001) Influence of one or two successive annual applications of organic fertilizers on the enzime activity of a soil under barley cultivation. Bioresour Technol 79:147–154. CrossRefGoogle Scholar
  38. Marschner H (1995) Mineral nutrition of higher plants, 2nd ed. Academic Press, San DiegoGoogle Scholar
  39. Mokolobate MS, Haynes RJ (2002) Increases in pH and soluble salts influence the effect that additions of organic residues have on concentrations of exchangeable and soil solution aluminum. Eur J Soil Sci 53:481–489. CrossRefGoogle Scholar
  40. Murphy J, Riley JP (1962) A modified single solution method for determination of phosphate in natural waters. Anal Chim Acta 27:31–36. CrossRefGoogle Scholar
  41. Neumann G, Römheld V (2012) Rhizosphere chemistry in relation to plant nutrition. In: Marschner P (ed) 3rd ed. Elsevier, San Diego, pp 347–368Google Scholar
  42. Okonkwo CI (2010) Effect of burning and cultivation on soil properties and microbial population of four different land use systems in Abakaliki. Res J Agric Biol Sci 6:1007–1014Google Scholar
  43. Olsen SR, Sommers LE (1982) Phosphorus. In: Page AL, Miller RH, Keeny DR (eds) Methods of soil analysis. Part 2. Chemical and microbiological properties, 2nd ed. ASA. SSSA, Madison, pp 403–430Google Scholar
  44. Ortínez-Alvarez A, Peralta O, Alvarez-Ospina H, Martínez-Arroyo A, Castro T, Páramo VH, Ruiz-Suárez LG, Garza J, Saavedra I, Espinoza ML, Vizcaya-Ruiz A, Gavilan A, Basaldud R, Munguía-Gillén JL (2018) Concentration profile of elemental and organic carbon and personal exposure to other pollutants from brick kilns in Durango, Mexico. Air Qual Atmos Health 11:285–300. CrossRefGoogle Scholar
  45. Palese MA, Giovannini G, Luchesi S, Dumontet S, Perucci P (2004) Effect to fire on soil C, N and microbial biomass. Agron. 24:47–53. CrossRefGoogle Scholar
  46. Pathan SI, Ceccherini MT, Pietramellara G, Puschenreiter M, Giagnoni L, Arenella M, Varanini Z, Nannipieri P, Renella G (2015) Enzyme activity and microbial community structure in the rhizosphere of two maize lines differing in N use efficiency. Plant Soil 387:413–424. CrossRefGoogle Scholar
  47. Paz-Ferreiro J, Trasar-Cepeda C, Leirós MC, Seoane S, Gil-Sotres F (2007) Biochemical properties of acid soils under native grassland in a temperate humid zone. N Z J Agric Res 50:537–548. CrossRefGoogle Scholar
  48. Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with their vital activities of some microbial species. Microbiology. 17:362–370Google Scholar
  49. Plante FA, Stone MM, McGill BW (2015) The metabolic physiology of soil microorganisms. In: Paul AE (ed) Soil microbiology, ecology, and biochemistry. Academic Press of Elsevier, Colorado, pp 245–272CrossRefGoogle Scholar
  50. Porta CJ, López-Acevedo RM, Roqueri DLC (2003) Edafología para la agricultura y el medio ambiente, 3a. ed. Ediciones Mundi-Prensa, MadridGoogle Scholar
  51. Rennie RJ (1981) A single medium for the isolation of acetylene reducing (dinitrogen-fixing) bacteria from soils. Can J Microb 27:8–14. CrossRefGoogle Scholar
  52. Resende AS, Xavier RP, de Oliveira OC, Urquiaga S, Alves BJR, Boddey RM (2006) Long-term effects of pre-harvest burning and nitrogen and vinasse applications on yield of sugar cane and soil carbon and nitrogen stocks on a plantation in Pernambuco, N.E. Brazil. Plant Soil 281:339–351. CrossRefGoogle Scholar
  53. Rodríguez FH, Rodríguez AJ (2011) Métodos de análisis de suelos y plantas. Criterios de interpretación. Editorial Trillas, S.A. de C.V. MonterreyGoogle Scholar
  54. Rodríguez-Rodríguez N, Rivera-Cruz MC, Trujillo-Narcía A, Almaráz-Suárez JJ, Salgado-García S (2016) Spatial distribution of oil and biostimulation through the rhizosphere of Leersia hexandra in degraded soil. Water Air Soil Pollut 227:319. 1org/. CrossRefGoogle Scholar
  55. Romanyà J, Casals P, Vallejo R (2001) Short-term effects of fire on soil nitrogen availability in Mediterranean grassland and shrublands growing in old fields. For Ecol Manag 147:39–53. CrossRefGoogle Scholar
  56. Sant’anna SAC, Fernandes MF, Ivo WMPM, Costa JLS (2009) Evaluation of soil quality indicators in sugarcane management in sandy loam soil. Pedosphere. 19:312–322. CrossRefGoogle Scholar
  57. SAS Institute Inc (2005) The SAS System for Windows, Release 8.01. SAS Institute Inc, CaryGoogle Scholar
  58. Sebastian SP, Udayasoorian C, Jayabalakrishnan RM (2009) Influence of amendments on soil fertility status of sugarcane with poor quality irrigation water. Sugar Tech 11:338–346. CrossRefGoogle Scholar
  59. Smith RN, Kishchuk EB, Mohn WW (2008) Effects of wildfire and harvest disturbances on forest soil bacterial communities. Appl Environ Microbiol 74:216–224. CrossRefGoogle Scholar
  60. Smith JL, Collins HP, Bailey VL (2010) The effect of young biochar on soil respiration. Soil Biol Biochem 42:2345–2347. CrossRefGoogle Scholar
  61. Sornpoon W, Bonnet S, Garivait S (2013) Effect of open burning on soil carbon stock in sugarcane plantation in Thailand. Int J Environ Ecol Eng 7:775–779Google Scholar
  62. Souza RA, Telles TS, Machado W, Hungria M, Filho JT, Guimaraes MF (2012) Effects of sugarcane harvesting with burning on the chemical and microbiological properties of the soil. Agric Ecosyst Environ 155:1–6. CrossRefGoogle Scholar
  63. Souza GS, Souza ZM, Silva RB, Barbosa RS, Araújo FS (2014) Effects of traffic control on the soil physical quality and the cultivation of sugarcane. Rev Bras Ci Solo 38:35–146. Google Scholar
  64. Štursová M, Baldrian P (2011) Effects of soil properties and management on the activity of soil organic matter transforming enzymes and the quantification of soil-bound and free activity. Plant Soil 338:99–110. CrossRefGoogle Scholar
  65. Sunday AA (2010) Effects of slash and burning on soil microbial diversity and abundance in the tropical rainforest ecosystem, Ondo State, Nigeria. Afr J Plant Sci 4:322–329 Article Number: 6AFBD9611961Google Scholar
  66. Tabatabai MA (1994) Soil enzymes. In: Weaver RW, Angle JS, Botttomley PS (eds) Methods of soil analysis, part 2. Microbiological and Biochemical Properties. Soil Science Society of America, Madison, WI, pp 775–833Google Scholar
  67. Velmourougane K, Venugopalan VM, Bhattacharyya T, Sarka D, Pal KD, Sahu A, Chandran P, Ray KS, Mandal C, Nair MK, Prasad J, Singh ES, Tiwary P (2013) Urease ativity in various agro-ecological sub-regions of black soil regions of India. Proc Natl Acad Sci India Sect B Biol Sci 83:513–524. CrossRefGoogle Scholar
  68. Violante A, Caporale AG (2015) Biogeochemical processes at soil-root interface. J Soil Sci Plant Nutr 15:422–448. Google Scholar
  69. Violante A, Pigna M, Cozzolino V, Huang PM (2011) Impact of soil physical, chemical and biological interactions on the transformation of metals and metalloids. In: Huang PM, Li Y, Summer ME (eds) Handbook of soil sciences resource of management and environmental impacts, Second edn. CRC Press, Taylor & Francis, Boca Raton, pp 155–171Google Scholar
  70. Walkley A, Black IA (1934) An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38. CrossRefGoogle Scholar
  71. Wang N, Li J-Y, Xu R-K (2009) Use of agricultural by-products to study the pH effects in an acid tea garden soil. Soil Use Manag 25:28–32. CrossRefGoogle Scholar
  72. Wood WA (1991) Management of crop residues following green harvesting of sugarcane in north Queenland. Soil Tillage Res 20:69–85. CrossRefGoogle Scholar
  73. Xiang X, Shi Y, Yang J, Kong J, Lin X, Zhang H, Zeng J, Chu H (2014) Rapid recovery of soil bacterial communities after wildfire in a Chinese boreal forest. Sci Rep 2014:3829. Google Scholar
  74. Yuan G, Theng BKG (2011) Clay-organic interactions in soil environments. In: Huang PM, Li Y, Summer ME (eds) Handbook of soil sciences resource of management and environmental impacts, Second ed. CRC Press, Taylor & Francis, Boca Raton, pp 62–77Google Scholar
  75. Zhao X, Xing GX (2009) Variation in the relationship between nitrification and acidification of subtropical soils as affected by the addition of urea or ammonium sulfate. Soil Biol Biochem 41:2584–2587. CrossRefGoogle Scholar

Copyright information

© Sociedad Chilena de la Ciencia del Suelo 2019

Authors and Affiliations

  • A. Trujillo-Narcía
    • 1
  • M. C. Rivera-Cruz
    • 2
    Email author
  • M. Magaña-Aquino
    • 1
  • E. A. Trujillo-Rivera
    • 3
  1. 1.Cuerpo Académico Energía y Medioambiente. Programa Educativo de QFB, Químico Farmacéutico BiólogoUniversidad Popular de la ChontalpaH. CárdenasMéxico
  2. 2.Laboratorio de Microbiología Agrícola y AmbientalColegio de Postgraduados Campus TabascoH. CárdenasMéxico
  3. 3.Science & Engineering Hall, Suite 8390The George Washington UniversityWashingtonUSA

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