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Lindane Bioremediation in Soils of Different Textural Classes by an Actinobacteria Consortium

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Abstract

Lindane is a highly chlorinated and recalcitrant insecticide, capable to accumulate in soil and groundwater. Despite lindane has been banned in many countries, numerous sites still remain contaminated. The present work studies the bioremediation of soils of different textures contaminated with lindane by bioaugmentation with a quadruple Streptomyces consortium. In the three evaluated soils, silty loam soil (SLS), sandy soil (SS), and clayey soil (CS), heterotrophic microbial populations increased during the 14 days of the assay and CFU counts were higher in bioaugmented than in non-bioaugmented soils. Lindane removal was detected in all contaminated treatments, with higher removal percentages in the bioaugmented microcosms (SS 70.3%, SLS 36.3%, and CS 30.7%), than in non-bioaugmented ones (SS 40.4%, SLS 9.3%, and CS 12.2%). The pesticide half-life decreased by 77.3, 50.3, and 10.7 days, in bioaugmented SLS, CS, and SS, respectively. Lindane had an inhibitory effect on soil enzyme activities such as dehydrogenase, fluorescein diacetate hydrolysis, acid and alkaline phosphatases and increased the catalase activity, in non-bioaugmented controls; however, no effect on urease activity was observed. Bioaugmentation of soil microcosms with actinobacteria increased all enzymatic activities. Finally, the survival of the four strains of the consortium was demonstrated at the end of the bioremediation assay. Bioremediation using the Streptomyces sp. A2-A5-A11-M7 consortium represents a promising tool to restore different types of soils contaminated with organochlorine pesticides.

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Acknowledgements

The authors would like to thank Mr. Guillermo Borchia, Mrs. Liliana Colombres, and the student Leandro Coronel for their technical assistance.

Funding

This study was financially supported by Secretaria de Ciencia, Arte e Innovación Tecnológica, Universidad Nacional de Tucumán (PIUNT D504); Agencia Nacional de Promoción Científica y Tecnológica (PICT 2013 No. 0141; PICT 2014 No. 2893); and Consejo Nacional de Investigaciones Científicas y Técnicas.

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Authors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Avenida Belgrano y Pasaje Caseros, 4000, Tucumán, Argentina

    Enzo E. Raimondo, Juan D. Aparicio, María S. Fuentes & Claudia S. Benimeli

  2. Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 491, 4000, Tucumán, Argentina

    Juan D. Aparicio

  3. Departamento de Ingeniería Química, Universidad de La Frontera, Avenida Francisco Salazar 01145, 4780000, Temuco, Chile

    Gabriela E. Briceño

  4. Núcleo de Desarrollo Científico Tecnológico, Universidad de La Frontera, Avenida Francisco Salazar 01145, 4780000, Temuco, Chile

    Gabriela E. Briceño

  5. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Catamarca, Belgrano 300, 4700, Catamarca, Argentina

    Claudia S. Benimeli

Authors
  1. Enzo E. Raimondo
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  2. Juan D. Aparicio
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  3. Gabriela E. Briceño
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  4. María S. Fuentes
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  5. Claudia S. Benimeli
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Correspondence to Claudia S. Benimeli.

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Raimondo, E.E., Aparicio, J.D., Briceño, G.E. et al. Lindane Bioremediation in Soils of Different Textural Classes by an Actinobacteria Consortium. J Soil Sci Plant Nutr 19, 29–41 (2019). https://doi.org/10.1007/s42729-018-0003-7

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  • DOI: https://doi.org/10.1007/s42729-018-0003-7

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