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Molecular characterization of Anopheles gambiae complex mosquitoes in rice agroecosystems in Anambra State, Nigeria

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The molecular characterization of Anopheles gambiae complex mosquitoes in rice agroecosystems in Anambra State was studied from January to December 2020. The study was carried out in rice farms randomly selected from four local government areas in Anambra State. Mosquito larvae were collected with a WHO (350ml) standard dipper at the sampling point of each of the selected rice farms twice every month. The larval samples were taken to the insectary of the National Arbovirus and Vectors Research Centre (NAVRC) to be reared into adults. The molecular characterization of sibling species of Anopheles gambiae complex was accomplished with Polymerase Chain Reaction (PCR). A total of 289 An. gambiae complex mosquitoes were subjected to PCR and identified. The species were identified as An. gambiae s.s., 144 (49.8%) and An. coluzzii. 145 (50.1%). These observations indicated that malaria transmission in the Anambra state is mainly carried out by the Anopheles sibling species. This enhances, sustains, and extends the malaria disease transmission in this geographical area.

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We would like to express our gratitude to the members of our research team, who worked tirelessly to collect the data for this study, and their valuable feedback and support throughout the writing process. We would also like to thank Dr. C. M. Egbuche at Nnamdi Azikiwe University Awka Anambra, Nigeria for analysing the data for this study. We would also like to thank the community heads and farm owners in Anambra State for the support and assistance they rendered to facilitate the successful completion of this study. Finally, we are grateful for the support and research facilities provided by the School of Biological Sciences, Universiti Sains Malaysia, Malaysia.

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Correspondence to Hadura Abu Hasan.

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Ukonze, C., Ezihe, E., Nzeukwu, C. et al. Molecular characterization of Anopheles gambiae complex mosquitoes in rice agroecosystems in Anambra State, Nigeria. Int J Trop Insect Sci 43, 1911–1918 (2023).

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