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Adult oviposition preference and larval performance of Cnaphalocrocis medinalis Guenee (Pyralidae: Lepidoptera) on transgenic Bt rice

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Abstract

The oviposition of gravid female moths of rice leaffolder, Cnaphalocrosis medinalis Guenee (Pyralidae: Lepidoptera) correlates with the host plant’s fitness for their offspring development. The preference for oviposition and subsequent larval performance of leaffolders were studied on transgenic rice plants expressing an insecticidal protein Cry2AX1 to understand the adult oviposition and larval development behaviour of rice leaffolders. Expression of Cry2AX1 protein in transgenic plants ranged between 18 ± 0.01 and 128 ± 0.06 ng/gram of fresh leaf tissues. The female adults did not exhibit preference for oviposition between transgenic and non-transgenic plants. However, significant differences in larval survival and adult emergence indicated the deleterious effect of transgenic plants on the growth and development of larval and adult stages. The larvae took a longer time for selection and folding of leaves on transgenic plants compared to wild-type counterparts. Besides, the folding length in the leaves of transgenic plants was shorter, exposing the larvae to biotic (parasitoids and predators) and abiotic factors.

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Abbreviations

Bt :

Bacillus thuringiensis

µg / g:

Microgram per gram

PCR:

Polymerase chain reactions

ELISA:

Enzyme Linked Immuno Sorbent Assay

ASD16:

Ambasamudram 16

TN1:

Taichung Native 1

CTAB:

Cetyl Trimethyl Ammonium Bromide

IPM:

Integrated Pest Management

DNA:

Deoxyribonucleic acid

h:

Hours

min:

Minutes

cm:

Centimeters

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Acknowledgements

The authors thank the Department of Biotechnology, Government of India (DBT-GOI) for funding. GR also thanks DBT for the fellowship.

Funding

This project was funded by the Department of Biotechnology, Government of India (DBT-GOI).

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Authors and Affiliations

  1. Department of Agricultural Entomology, Centre for Crop Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    Gothandaraman Rajadurai & Narayanasamy Natarajan

  2. Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    Duraialagaraja Sudhakar, Shanmugam Varanavasiappan & Varatharajalu Udayasuriyan

  3. Directorate of Research, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    Natarajan Balakrishnan

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  1. Gothandaraman Rajadurai
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  2. Duraialagaraja Sudhakar
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  3. Shanmugam Varanavasiappan
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  5. Varatharajalu Udayasuriyan
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  6. Narayanasamy Natarajan
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Contributions

DS, NN, and GR designed the experimental layout. GR performed the experiment. SV, NB, and UV helped in the planning of the experiment. DS, NN, and GR prepared the manuscript. SV critically revised the article. All the authors read and approved the final manuscript.

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Correspondence to Narayanasamy Natarajan.

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Rajadurai, G., Sudhakar, D., Varanavasiappan, S. et al. Adult oviposition preference and larval performance of Cnaphalocrocis medinalis Guenee (Pyralidae: Lepidoptera) on transgenic Bt rice. Int J Trop Insect Sci 43, 1037–1048 (2023). https://doi.org/10.1007/s42690-023-01026-2

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