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Ultrasturucture of Aedeagus and Spermatheca of type species of Hypocassida Weise, 1893 (Chrysomelidae: Cassidinae: Cassidini) and their taxonomic significance

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Abstract

Aedeagus and spermatheca of the type species Hypocassida subferruginea (Schrank) of the Palaearcticgenus Hypocassida Weise have been studied and figured on the base of 20 and 24 specified characters respectively under both stereo microscope and SEM for the first time. Contrary of popular acceptance, it is demonstrated that the characteristics of the aedeagal and spermathecal structures, which can be obtained with SEM studies especially, can be used at almost all taxonomic level diagnostically, however, will have different values ​​for different taxonomic categories. As a result of this study, in aedeagi, especially seven diverse characters (including characteristics of apex in dorsal view, general shape in lateral view, etc..), and in spermathecae, vasculum, ampulla, ductus glandula auxiliaris and ductus spermatheca are found generally constant within species, but distinct and useful in comparison with other species in the tribe Cassidini. Eventually, the aedeagal and spermathecal structure of the type species was found to be distinctive not only at the species level but also at the generic level. Accordingly, as an important output, the taxonomic position of Hypocassida is confirmed again on the base of aedeagal and spermathecal structures of its type species.

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References

  • Aslan EG, Kaya ÖD, Ünal E (2020) Contributions to the knowledge of leaf beetle (Coleoptera: Chrysomelidae) fauna in Elazığ, Erzincan and Tunceli provinces, Turkey. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi 11(Ek Sayı 1):273–280

  • Ataş F, Özdikmen H, Bal N, Amutkan Mutlu D, Suludere Z (2019a) A SEM study on aedeagus and spermatheca of Cassida seraphina Ménétries, 1836 (Coleoptera: Chrysomelidae: Cassidinae) from Turkey. Munis Entomol Zool 14(2):395–411

    Google Scholar 

  • Ataş F, Özdikmen H, Bal N, Amutkan MD, Suludere Z (2019b) A SEM study on aedeagus and spermatheca of Cassida hablitziae Motschulsky, 1838 (Coleoptera: Chrysomelidae: Cassidinae) from Turkey. Munis Entomol Zool 14(2):519–529

    Google Scholar 

  • Bechyne J (1944) Cassididae a Hispidae v Cechach a na Morave. Entomol Listy 7:74–86

    Google Scholar 

  • Biondi M, Daccordi M, Regalin R, Zampetti M (1995a) Coleoptera Polyphaga XV (Chrysomelidae, Bruchidae). In: Minelli A, Ruffo S, La Posta S (eds.), Checklist delle specie della fauna italiana, 60, Edizioni Calderini, Bologna, p 13–34

  • Biondi M, Regalin R, Daccordi M, Poggi R (1995b). I Crisomelidi (esclusi Alticini) delle isole circumsarde. In: Ricerche zoologiche della nave oceanografica “Minerva” (C.N.R.) sulle isole circumsarde. XXV. Ann Mus Civ Stor Nat Genova 90:629–651

  • Boheman CH (1856) Catalogue of coleopterous insects in the collection of the British Museum, Part IX. Cassididae, London

    Google Scholar 

  • Bordy B (2000) Coléopteres Chrysomelidae (Première édition). Hispinae et Cassidinae. Faune de France 85:250

  • Bordy B (2005) Sur la biologie de Hypocassida meridionalis (Suffrian, 1844) (Coleoptera, Chrysomelidae, Cassidinae). Le Coleopteriste 8(2):117–121

    Google Scholar 

  • Bordy B (2008) Identification des especes du complexe Hypocassida subferruginea (Schrank, 1776) (Coleoptera, Chrysomelidae). Nouv Rev Ent (N.S.) 25:45–59

  • Bordy B (2009) Compléments à la première édition (2000). Coléopteres Chrysomelidae. Hispinae et Cassidinae. Faune de France 85(Seconde édition mise à jour):19

  • Bordy B, Doguet S (1987) Contribution a la connaissance des Cassidinae de France. Etude de leur spermatheque (Coleoptera, Chrysomelidae). Nouvelle Revue d’Entomologie (N.S.) 4:161–176

  • Borowiec L (1999) A world catalogue of the Cassidinae (Coleoptera: Chrysomelidae). Biologica Silesiae, Wrocław, p 476

  • Borowiec L (2000) Hypocassida convexipennis, a new species from Iran (Coleoptera: Chrysomelidae: Cassidinae). Genus 11:601–605

    Google Scholar 

  • Borowiec L (2007) Two new species of Cassida Linnaeus, 1758 (Coleoptera: Chrysomelidae: Cassidinae) from Madagascar and notes on subgenera of the genus Cassida. Zootaxa 1586:47–58

    Article  Google Scholar 

  • Borowiec L, Opalińska S (2007) The structure of the spermathecae of selected genera of Stolaini and Eugenysini (Coleoptera: Chrysomelidae: Cassidinae) and its taxonomic significance. Ann Zool 57:463–479

    Google Scholar 

  • Borowiec L, Pomorska J (2009) The structure of the spermathecae of the genus Stolas (Coleoptera: Chrysomelidae: Cassidinae: Mesomphaliini) and its taxonomic significance. Ann Zool 59:201–221

    Article  Google Scholar 

  • Borowiec L, Sekerka L (2010) Cassidinae: pp. 64–65, 368–390. In: Löbl I, Smetana A (eds) Catalogue of Palaearctic Coleoptera, vol 6. Apollo Books, Chrysomeloidea, p 924

    Google Scholar 

  • Borowiec L, Skuza M (2004) The structure of spermatheca in the genus Chelymorpha Chevrolat, 1837 (Coleoptera: Chrysomelidae: Cassidinae) and its taxonomic significance. Ann Zool 54:439–451

    Google Scholar 

  • Borowiec L, Świętojańska J (2001) Revision of Cassida litigiosa group from southern Africa (Coleoptera: Chrysomelidae: Cassidinae). Annales Zoologici, Warszawa 51:153–184

    Google Scholar 

  • Borowiec L, Świętojańska J (2019) Cassidinae of the World – an Interactive Manual (Coleoptera: Chrysomelidae). http://www.cassidae.uni.wroc.pl/catalog%20internetowy/index.htm. Accessed 21 Dec 2019

  • Brovdij VM (1983) Zhuki-listoidi, shchitonoski i shiponoski. In: Fauna Ukraini, Tom 19, vyp. 20, Kiev, p 188

  • Chaboo CS (2002) First report of immatures, genitalia and material care in Eugenysa columbiana (Boheman) (Coleoptera: Chrysomelidae: Cassidinae: Eugenysini). Coleopt Bull 56:50–67

    Article  Google Scholar 

  • Chujô M (1934) Studies on the Chrysomelidae in the Japanese Empire, V. Sylvia 5:145–182

    Google Scholar 

  • Ekiz AN, Şen İ, Aslan EG, Gök A (2013) Checklist of leaf beetles (Coleoptera: Chrysomelidae) of Turkey, excluding Bruchinae. J Nat Hist 47(33–34):2213–2287

    Article  Google Scholar 

  • Escalera MMDELA (1914) Los Coleópteros de Marruecos. Trab Mus Nac Cien Nat Madrid 11:553

  • Fabricius JC (1781) Species Insectorum eorum differentias specificas, synonyma auctorum, loca natalia, metamorphosis adiectis observationibus, descriptionibus. I. Hamburgi et Kilonii, VIII, p 552

  • Gök A, Bostan K (2020) The first faunistic data on the leaf beetles (Coleoptera: Chrysomelidae) of 26 Ağustos Nature Park, Afyonkarahisar, Turkey. J Entomol Res Soc 22(1):83–99

    Google Scholar 

  • Gök A, Turantepe E (2019) Additions to the fauna of Chrysomelidae (Coleoptera) from Hatila Valley National Park (Artvin, Turkey), with notes on host plant preferences and zoogeographic evaluations. Caucasian Entomol Bullet 15:135–146

    Article  Google Scholar 

  • Gruev B, Tomov V (1986) Coleoptera, Chrysomelidae, chast II, Chrysomelinae, Galerucinae, Alticinae, Hispinae, Cassidinae. In: Fauna na Bulgaria, vol. 16, Sofia, p 388

  • Gruev B, Tomov V (1998) Coleoptera, Chrysomelidae. In: Catalogus Faunae Bulgaricae, 3. Pensoft Publ., Sofia-Moscow, p 160

  • Gruev B, Tomov V (2007) A distributional atlas and catalogue of the leaf beetles of Bulgaria. Coleoptera, Chrysomelidae. In: Zoocartographia Balcanica, vol 3. Pensoft Publ, Sofia-Moscow, p 350

    Google Scholar 

  • Gyllenhal L (1813) Insecta Suecica. Classis I. Coleoptera sive Eleuterata, Tomus I, pars III. Scaris, p 734

  • Hincks WD (1952) The genera of the Cassidinae (Coleoptera: Chrysomelidae). Trans Royal Entomol Soc London 103:327–358

    Article  Google Scholar 

  • Irurzin JR, Bustamante JA (2018) Hypocassida grossepunctata Bordy, 2008 nuevo crisomélido para la fauna ibérica y lista preliminar de los Cassidinae de la España Peninsular (Coleoptera: Chrysomelidae). Heteropterus Revista De Entomología 18(2):237–240

    Google Scholar 

  • Laicharting JNE (1781) Verzeichniss und Beschreibung der Tyroler-Insecten. I Theil. Käferartige Insecten. Zürich, 12:248

  • Linnaeus C (1758) Systema Naturae, sive regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Editio Decima, reformata. I. Holmiae, IV, p 824

  • Lopatin IK (1977) Zhuki listoyedy Sredniei Azii i Kazakhstana. In: Opredeliteli po faunie SSSR, 113, Leningrad, p 269

  • Lopatin IK, Kulenova KZ (1986) Zhuki listoyedy (Coleoptera, Chrysomelidae) Kazakhstana. Opredelitel'. Alma-Ata, p 198

  • López-Pérez S, Zaragoza-Caballero S, Chaboo CS (2016) Revision of Ogdoecosta Spaeth 1909 with description of Ogdoecosta paraflavomaculata López-Pérez, sp. nov. (Coleoptera: Chrysomelidae: Cassidinae: Mesomphaliini). Zootaxa 4179:410–440

    Article  PubMed  Google Scholar 

  • López-Pérez S, Zaragoza-Caballero S, Ochoterena H, Morrone JJ (2018) A phylogenetic study of the worldwide tribe Cassidini Gyllenhal, 1813 (Coleoptera: Chrysomelidae: Cassidinae) based on morphological data. Syst Entomol 43:372–386

    Article  Google Scholar 

  • Marseul MS (1868) Descriptions des especes nouvelles. Abeille 5:171–217

    Google Scholar 

  • Medvedev LN (1982) Listoyedy MNR. Opredelitel. Nauka, Moskva, 1982, p 303.

  • Medvedev LN (1992) 105. Sem. Chrysomelidae - listoedy. In: Key to the insects of the Far East of SSSR, Nauka, Sankt-Petersburg, p 533–602

  • Özdikmen H (2011) A comprehensive contribution for leaf beetles of Turkey with a zoogeographical evaluation for all Turkish fauna (Coleoptera: Chrysomelidae). Munis Entomol Zool 6(2):540–638

    Google Scholar 

  • Özdikmen H, Kaya G (2014) Chorotype identification for Turkish Chrysomeloidea (Coleoptera) Part I – Chrysomelidae: Hispinae and Cassidinae. Munis Entomol Zool 9(1):58–70

    Google Scholar 

  • Özdikmen H, Bal N, Amutkan Mutlu D, Suludere Z (2020a) A SEM study on aedeagus and spermatheca of Cassida nebulosa Linnaeus, 1758 (Coleoptera: Chrysomelidae: Cassidinae) from Turkey. Munis Entomol Zool 15(1):252–261

    Google Scholar 

  • Özdikmen H, Şahin CD, Bal N (2020b) A new species of Cassida Linnaeus, 1758 from Turkey (Chrysomelidae: Cassidinae). Microsc Res Tech 83(10):1191–1177

    Google Scholar 

  • Özdikmen H, Bal N, Amutkan Mutlu D, Suludere Z (2021a) Comparative ultrastructural analysis to six subgenera of Cassida Linnaeus, 1758 (Coleoptera: Chrysomelidae: Cassidinae) based on spermatheca of the type species and its taxonomic significance. Trans Am Entomol Soc 147:67–99

    Article  Google Scholar 

  • Özdikmen H, Bal N, Amutkan Mutlu D, Suludere Z (2021b) Comparative ultrastructural analysis to seven subgenera of Cassida Linnaeus, 1758 (Coleoptera: Chrysomelidae: Cassidinae) based on aedeagus of the type species and its taxonomic significance. Trans Am Entomol Soc XX(X):XXX-XXX (in press)

  • Petitpierre E, Carreras I, Gómez-Zurita J (1998) Cytogenetic analysis of European Cassida (Coleoptera, Chrysomelidae). Hereditas 128:1–8

    Article  Google Scholar 

  • Sassi D (1994) Le Cassidinae appeniniche del Museo di Storia Naturale di Verona (Coleoptera, Chrysomelidae). Boll Mus Civ Stor Nat Verona 18:53–90

    Google Scholar 

  • Sassi D, Borowiec L (2006) Cassida inopinata, a new species from Italy and Balkan Region (Coleoptera: Chrysomelidae: Cassidinae). Genus 17(4):545–560

    Google Scholar 

  • Schrank F (1776) Beiträge zur Naturgeschichte. Augsburg, p 140

  • Seeno TN, Wilcox JA (1982) Leaf beetle genera (Coleoptera: Chrysomelidae). Entomography 1:1–221

    Google Scholar 

  • Spaeth F (1914) Chrysomelidae: 16. Cassidinae. In: W. Junk, S. Schenkling, Coleopterorum Catalogus, Pars 62, Berlin, p 182

  • Spaeth F (1924) Cassidinae. In: Voyage de CH. Alluaud et R. Jeannel en Afrique orientale (1911–1912). Résultats scientifiques. Coleoptera XVIII:275–363

  • Spaeth F, Reitter E (1926) Bestimmungs-Tabellen der europäischen Coleopteren. 95 Heft. Cassidinae der palaearktischen Region. Troppau, p 68

  • Steinhausen W (1950) Vergleichende Morphologie Biologie Ökologie der Entwicklungsstadien der in Niedersachsen heimischen Schildkäfer (Cassidinae Chrysomelidae Coleoptera) und deren Bedeutung für die Landwirtschaft, Doktor Diss. Techn. Hochsch. zu Braunschweig, p 71

  • Suenaga H (2013) Notes on Cassida ferruginea and Cassida mongolica in Japan, with descriptions of their reproductive systems (Coleoptera: Chrysomelidae: Cassidinae). Genus 24:325–333

    Google Scholar 

  • Suffrian E (1844) Fragmente zur genaueren Kenntnis deutscher Käfer. 4. Cassida l Stettin Entomologische Zeitung 5:270–287

    Google Scholar 

  • Suzuki K (1988) Comparative morphology of the internal reproductive system of the Chrysomelidae (Coleoptera). In: Jolivet P, Petitpierre E, Hsiao TH (eds) Biology of the Chrysomelidae. Kluwer, Dordrecht, Boston, London, pp 317–355

    Chapter  Google Scholar 

  • Warchałowski A (2000) Chrysomelidae. Stonkowate, część VII (Insecta: Coleoptera). In: Fauna Polski-Fauna Poloniae, vol. 22, Polskie Towarzys two Entomologiczne, Warszawa, p 359

  • Warchalowski A (2003) Chrysomelidae: The Leaf Beetles of Europe and the Mediterranean Area. Warszawa: Natura optima dux Foundation, p 600

  • Warchalowski A (2010) The Palaearctic Chrysomelidae. Identification keys, vol. 1 & 2. Warszawa: Natura optima dux Foundation, p 1212

  • Weise J (1893) Naturgeschichte der Insecten Deutschland. Erste Abtheilung Coleoptera. Sechster Band. Berlin, Nicolaische Verlags-Buchhandlung R. Stricker, XIV, p 1161

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Acknowledgements

We are very grateful to Dr. Didem Coral Şahin (Turkey) for providing some specimens studied in this work and for her valuable assistance.

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The authors have no affiliation with any organization with a direct or indirect financial interest in the subject matter discussed in the manuscript.The authors declare they have no financial interests.

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  1. Science Faculty, Department of Biology, Gazi University, 06500, Ankara, Turkey

    Hüseyin Özdikmen, Neslihan Bal, Damla Amutkan Mutlu & Zekiye Suludere

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  1. Hüseyin Özdikmen
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Özdikmen, H., Bal, N., Amutkan Mutlu, D. et al. Ultrasturucture of Aedeagus and Spermatheca of type species of Hypocassida Weise, 1893 (Chrysomelidae: Cassidinae: Cassidini) and their taxonomic significance. Int J Trop Insect Sci 42, 2337–2349 (2022). https://doi.org/10.1007/s42690-022-00756-z

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