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Electroantennography and behavioral studies of Eublemma amabilis [Moore] and Pseudohypatopa pulverea [Meyr] in relation to volatiles of lac insect (Kerria lacca Kerr.) and its associated products

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Abstract

Chemical communication between the lac insect associated products and the lac predators were evaluated under the laboratory condition. Semiochemicals were identified and comparative analysis carried out for different stages of lac insect and its associated products. Six major compounds viz. decane, dodecane, tetradecane, heptadecane, eicosane and octacosyl acetate constituted 78%, 79% and 85% in lac insect whole body, lac resin and lac wax extracts, respectively. Electroantennograms (EAGs) responses of adult male and female of lac insect predator, Eublemma amabilis to different lac insect extracts and its associated products (LIAPs) revealed higher responses of predator male and female to lac insect whole body extract (-0.731 and -1.182 mV, respectively) than resin, wax, crawler and lac insect female extracts. Among both the sexes of another lac insect predator Pseudohypatopa pulverea, EAGs response was significantly higher in female towards lac insect whole body (-1.059 mV) than resin, wax, adult female and crawler extracts than P. pulverea males. Both E. amabilis and P. pulverea exhibited high level of sensitivity to lac insect whole body extracts with different concentrations viz., 1000 ppm, 5000 ppm and 10000 ppm than identified semiochemicals viz., Decane, Hexadecane, Nonadecane, Eicosane and hexane. Orientation response of E. amabillis and P. pulverea through “Y” tube olfactometer revealed higher attraction of P. pulverea than E. amabillis.

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Data availability

The data that support the findings of this study are available from the author upon reasonable request.

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Acknowledgements

The authors are grateful to Director, ICAR- Indian Institute of Natural Resins and Gums, Ranchi and Dr Chitra Srivastava, Former Head, Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi for providing necessary facilities.

Funding

The funding for the study was provided by ICAR-Indian Institute of Natural Resins and Gums.

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Authors and Affiliations

  1. ICAR-Indian Institute of Natural Resins and Gums, Ranchi, Jharkhand, 834 010, India

    Arumugam Mohanasundaram, Kewal Krishan Sharma, Naiyar Naaz, Rashmi Mishra & Upnit Shree

  2. ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, India

    Anees Kaprakkaden

  3. ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    Suresh M. Nebapure

Authors
  1. Arumugam Mohanasundaram
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  2. Anees Kaprakkaden
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  3. Suresh M. Nebapure
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  4. Kewal Krishan Sharma
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  5. Naiyar Naaz
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  6. Rashmi Mishra
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Contributions

Dr.Arumugam Mohanasundram Inoculation of lac insect on host plant, Semiochemicals extraction from LIAPs, Olfactometer studies and analysed the data. Dr Anees Kaprakkaden GC–MS profiling, Suresh M Nebapure EAG studies, Dr. Kewal Krishan Sharma guided in the experimental plan and set up and assisted in manuscript preparation. Naiyar Naaz, Rashmi Mishra and Upnit Shree collected and observed the data.

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Correspondence to Arumugam Mohanasundaram.

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Mohanasundaram, A., Kaprakkaden, A., Nebapure, S.M. et al. Electroantennography and behavioral studies of Eublemma amabilis [Moore] and Pseudohypatopa pulverea [Meyr] in relation to volatiles of lac insect (Kerria lacca Kerr.) and its associated products. Int J Trop Insect Sci 42, 2313–2324 (2022). https://doi.org/10.1007/s42690-022-00754-1

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