Skip to main content
Log in

“Bumble bees” invitro rearing methods – a review

  • Mini-review
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

Bumble bees belong to the genus Bombus, order Hymenoptera and family Bombidae, which includes approximately 250 species all over the world, out of which 48 species are found in India. Bumble bees are effective pollinators with high pollination efficiency and increasing demand of pollination services has highlighted the concept of commercialization of these pollinators. They generally have an annual life cycle with three castes viz., queen, workers and drones; only the queen undergoes diapause during winters. The oviposition rate and colony initiation percentage of the bumble bees are affected by different factors viz., foundation queen, feed, hygienic conditions, nesting material and environmental conditions. Amongst these, nesting material as well as feeding methods are the most important factors that affect the success rate and queen acceptance. Hence, it is very important to know which nesting material and feed is highly accepted by queens for colony initiation and development. The present review summarizes the literature about different domiciles and feed used for invitro rearing of bumble bees and their effect on colony development.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Beekman M, Stratum VP (2000) Does the diapause experience of bumblebee, Bombus terrestris queens effect colony characteristics. Ecol Entomol 25:1–6

    Google Scholar 

  • Brown S, Rains J (2006) Bumble bee (Bombus impatiens) pollination of field crops in the state of California. California Environmental Quality Act Initial Study and mitigated negative declaration. September (2006) Bumble bee - California Department of Food and Agriculture. Koppert Biological Systems, Inc., Ardea consulting, pp 59

    Google Scholar 

  • Bucankova A, Ptacek V (2010) Experiences in rearing of Bombus lucorum L. (Hymenoptera: Apoidea) in captivity. Uroda 12 Vedeckapriloha 621–624

  • Bucankova A, Ptacek V (2012) A test of Bombus terrestris coccon and other common methods for nest initiation in B. lapidaries and B. hortorum. J Apic Sci 56:37–47

    Google Scholar 

  • Buchmann STL, Hurley JP (1978) A biophysical model for buzz pollination in angiosperms. J Theor Bio 72:639–657

    CAS  Google Scholar 

  • Chauhan A (2011) Refinement of bumble bee rearing technology and its use in cucumber pollination. M.Sc. Thesis. Department of Entomology, Dr YSP University of Horticulture and Forestry, Nauni, Solan, 152

  • Chauhan A, Katna S, Rana BS (2013a) Life cycle of bumble bee Bombus haemorrhoidalis Smith in Himachal Pradesh. Insect Environ 19:183–186

    Google Scholar 

  • Chauhan A, Katna S, Rana BS, Miyan HV (2013b) Field establishment of artificially reared bumble bee (Bombus haemorrhoidalis Smith) colonies in Himachal Pradesh. Insect Environ 19:159–161

    Google Scholar 

  • Chauhan A, Rana BS, Katna S (2014) Successful rearing of bumble bee, Bombus haemorrhoidalis Smith year-round in Himachal Pradesh in India. Int J Curr Res 6:10891–10896

    Google Scholar 

  • Dayal K, Rana BS (2004) Record of domestication of Bombus species (Hymenoptera: Apidae) in India. Insect Environ 10:64–65

    Google Scholar 

  • Devi D (2019) Studies on nesting material and carbon dioxide narcosis on domicilation of bumble bee (Bombus haemorrhoidalis Smith). M.Sc. Thesis. Department of Entomology, Dr YSP University of Horticulture and Forestry, Nauni, Solan, 68p

  • Donovon BJ (2001) Calculated value of nests of long tongued bumble bee Bombus hortorum, for pollination of tetraploid red clover Trifolium prantese. Acta Hortic 521:293–296

    Google Scholar 

  • Free JB (1993) Insect pollination of crops, 2nd edn. Academic Press, London

    Google Scholar 

  • Friden F (1965) Preliminar redogorelse for forsoken med humledomesticering. (Personal communication)

  • Frison TH (1927) The fertilization and hibernation of queen bumblebees under controlled conditions (Bremidae: Hym.). J Econ Entomol 20:522–526

    Google Scholar 

  • Fye RE, Medler JT (1954) Field domiciles for bumble bees. J Econ Entomol 47:672–676

    Google Scholar 

  • Groot AP (1953) Protein and amino acid requirements of the honeybee (Apis mellifera L). Laboratory of Comparative Physiology Utrecht, 197–285

  • Haydak MH (1970) Honey bee (Hymenoptera: Apidae) nutrition. Ann Rev Entomol 15:143–156

    Google Scholar 

  • Hobbs GA, Nummi WO, Virostek JF (1962) Managing colonies of bumble bees for pollination purposes. Can Entomol 94:1121–1132

    Google Scholar 

  • Holm SN (1960) Experiments on the domestication of bumble bees (Bombus Latr.) in particular Bombus lapidarius L. and B. terrestris L. Royal Veterinary and Agricultural College, Copenhagen, Yearbook pp. 1–19

  • Hughes MG (1996) Commercial rearing of bumblebees. In: Bumble bees for pleasure and profit (Matheson A ed.). IBRA, Cardiff, United Kingdom, pp. 40–47

  • Imran M, Ahmad M, Naeem M, Mahmood K, Nasir M, Sheikh UAA (2017a) Are colony developmental stages of bumblebee, Bombus terrestris (Hymenoptera: Apidae) affected by different concentrations of sugar and honey solutions. Int J Indus Entomol 34:1–9

    Google Scholar 

  • Imran M, Ahmad M, Naeem M, Nasir MF, Bodlah I, Nasir M, Sheikh UAA (2017b) Effect of different types of boxes on rearing of bumble bee, Bombus terrestris. Pak J Zool 49:177–182

    CAS  Google Scholar 

  • Imran M, Ahmad M, Nasir MF, Saeed S (2015) Effect of different nest box materials on the mating of European bumblebee, Bombus terrestris (Hymenoptera: Apidae) under controlled environmental conditions. Pak J Zool 47:241–247

    Google Scholar 

  • Iwasaki M (1995) Introduction of commercial bumblebees into Japan. Honeybee Sci 16:17–21

    Google Scholar 

  • Jie W, Wenjun P, Jiandong A, Zhanban G, Yueming T, Jilian L (2005) Techniques for year-round rearing of Bombus terrestris L. (Hymenoptera, Apoidea) in China. J Apic Sci 49:65–69

    Google Scholar 

  • Johnson SA, Tompkins MM, Tompkins H, Colla SR (2019) Artificial domicile use by bumble bees (Bombus; Hymenoptera: Apidae) in Ontario, Canada. J Ins Sci 19:1–5

    Google Scholar 

  • Karsli BA, Gurel F (2013) Effect of CO2 narcosis on the onset of oviposition and colony foundation success of post diapausing bumblebee (Bombus terrestris) queens. J Vet Med 19:221–224

    Google Scholar 

  • Kashyap L (2007) Domicilation of bumble bees (Bombus sp.), and to study resource partitioning with honey bees. M.Sc. Thesis. Department of Entomology, Dr YSP University of Horticulture and Forestry, Nauni, Solan, 73

  • Knee WJ, Medler JT (1965) The seasonal size increase of bumble bee workers (Hymenoptera: Bombus). Can Entomo l97:1149–1155

  • Kwon YJ (2008) Bombiculture: A fascinating insect industry for crop pollination in Korea. Entomol Res 38:66–70

    Google Scholar 

  • Lhomme P, Sramkova A, Kreuter K, Lecocq T, Rasmont P, Ayasse M (2013) A method for year-round rearing of cuckoo bumblebees (Hymenoptera: Apoidea: Bombus subgenus Psithyrus). Ann Soc Entomol Fr 49:117–125

    Google Scholar 

  • Lindhard K (1912) Humlebiensomhusdyr. Spredtetraek of nagledanskehumlebiartersbiologi. Tidsskr Landbr Planteavl 19:335–352

    Google Scholar 

  • Macfarlane RP, Griffin RP, Read PEC (1983) Bumble bee management options to improve grassland pawera red clover seed yields. Proc N Z Grassland Assoc 44:47–53

    Google Scholar 

  • Macfarlane RP, Van den Ende HJ, Griffin RP (1990) Pollination needs of the grassland pawera red clover. International Symposium of Pollination 6th in press

  • Munn J (1998) Helping bumble bee with Bombus nest boxes. Bee World 79:44–48

    Google Scholar 

  • Nayak RK (2018) Studies on bumble bee pollination in kiwifruit (Actinidia deliciosa Chev.). M.Sc. Thesis. Department of Entomology, Dr YSP University of Horticulture and Forestry, Nauni, Solan, 94p

  • Ono M, Mitsuhata M, Sasaki M (1994) Use of introduced Bombus terrestris workers helpers for rapid development of Japanese native Bombus hypocrite colonies (Hymenoptera: Apidae). Appl Entomol Zool 29:413–419

    Google Scholar 

  • Owen RE (2016) Rearing bumble bees for research and profit: Practical and ethical considerations. InBeekeeping and Bee Conservation - Advances in Research. pp. 226–242. https://doi.org/10.5772/63048

  • Pehling D (2002) Coffee can cottages for bumble bees (and other critters). Scarabogram 262:2–3

    Google Scholar 

  • Plath OE (1923) Breeding experiments with confined Bremus (Bombus) queens. Biol Bull Mar Biol Lab Woods Hole 45:325–341

    Google Scholar 

  • Plowright RC, Jay SC (1966) Rearing of bumble bee colonies in captivity. J Apic Res 5:155–165

    Google Scholar 

  • Plowright RC, Laverty TM (1987) Bumble bees and crop pollination in Ontario. Proc Entomol Soc Ontario 118:155–160

    Google Scholar 

  • Poissonnier LA, Jackson AL, Tanner CJ (2015) Cold and CO2 narcosis have long-lasting and dissimilar effects on Bombus terrestris. Insectes Soc 62:291–298

    Google Scholar 

  • Pomeroy N, Plowright RC (1980) Maintenance of bumble bee colonies in observation hives (Hymenoptera: Apidae). Can Entomol 112:321–326

    Google Scholar 

  • Pridal A, Hofbauer J (1996) Laboratory rearing and nutrition of young queens of bumblebee (Bombus terrestris L.) from emergence to diapause. Sci Stud Troubsko 14:125–131

    Google Scholar 

  • Pridal A, Hofbauer J (1998) Activation of laboratory-reared bumblebee queens (Hymenoptera: Apidae, Bombus spp.). Acta Univ Agric Silvic Mendel Brun 1:79–84

    Google Scholar 

  • Ptacek V (1985) Testing three methods of bumblebee rearing. SB Ved Prac VSUP Troubsko 9:59–67

    Google Scholar 

  • Ptacek V, Drobna J (2006) Rearing and using bumblebees for pollination of forage legume genetic resources. Second short course of pollination of horticultural plants. CIFA. La Mojonera, Spain, pp 139–153

    Google Scholar 

  • Ptacek V, Votavova A, Komzakova O (2015) Experiences in rearing common carder bees (Bombus pascuorum Scop.), with some notes on three similar species: Shrill carder bee (Bombus sylvarum L.), red shanked carder bee (Bombus ruderarius Mull.) and brown banded carder bee (Bombus humilis L.) (Hymenoptera: Apidae). Acta Univ Agric Silvic Mendel Brun 63:1535–1544

    Google Scholar 

  • Rasmont P, Regali A, Ings TC, Lognay G, Baudart E, Marlier M, Delcarte E, Viville P, Marot C, Falmagne P, Veriiaegiie JC, Chittka L (2005) Analysis of pollen and nectar of Arbutus unedo as a food source for Bombus terrestris (Hymenoptera: Apidae). J Econ Entomol 98:656–663

    CAS  PubMed  Google Scholar 

  • Ribeiro MF, Duchateau MJ, Velthuis HHW (1996) Comparison of the effects of two kinds of commercially available pollen on colony development and queen production in the bumble bee Bombus terrestris L (Hymenoptera; Apidae). Apidologie 27:133–144

    Google Scholar 

  • Röseler PF (1977) Rearing bumblebee colonies. Proc 8th Int Congr IUSSI Wageningen 312

  • Röseler PF (1979) Hummelzucht für den Erwerbsobstbau? Erwerbsobstbau 21:177–178

    Google Scholar 

  • Roseler PF (1985) A technique for year-round rearing of Bombus terrestris (Apidae, Bombini) colonies in captivity. Apidologie 16:165–170

    Google Scholar 

  • Roulston TH, Cane JH, Buchmann SL (2000) What governs protein content of pollen: pollinator major and minor floral choices of bumble bees 105 preferences, pollen-pistil interactions, or phylogeny? Ecological Monographs 70:617–643

    Google Scholar 

  • Saini MS, Ghattor HS (2007) Taxonomy and food plants of some bumble bee species of Lahaul and Spiti valley of Himachal Pradesh. Zoo’s Print J 22:2648–2657

    Google Scholar 

  • Sakagami SF (1966) Techniques for the observation of behaviour and social organization of stingless bees by using a special hive. Pap Avulsos de Zool, Sao Paulo 19:151–162

    Google Scholar 

  • Sevensson B, Lagerlof J, Sevensson BG (2000) Habitat preference of nest seeking bumblebees (Hymenoptera: Apidae) in an agricultural landscape. Agric Ecosyst Environ 77:247–255

    Google Scholar 

  • Sharma HK, Thakur S, Rana K, Thakur M (2018) Small scale rearing of Bombus haemorrhoidalis Smith. Pharma Innov 7:41–45

    Google Scholar 

  • Sladen FWL (1912) The bumble bee life history and how to domesticate it. Mac Millan, London, pp 253

    Google Scholar 

  • Strange JP (2010) Nest initiation in three North American bumble bees (Bombus): Gyne number and presence of honey bee workers influence establishment success and colony size. J Insect Sci 10:130p

    Google Scholar 

  • Tasei JN (1994) Effect of different narcosis procedures on initiating oviposition of pre-diapausing Bombus terrestris queens. Entomol Exp Appl 72:273–279

    Google Scholar 

  • Thakur RK (2002) First attempt to study nest architecture and domiciliation of bumble bee (Bombus haemorrhoidalis) in India. In: 6th Asian Apicultural Association International Conference & World Apiexpo Banglore – India 24 Feb – 1 Mar, pp 172

  • Thakur RK (2006) Bumble bee flora and domestication of bumble bee (Bombus spp.) under mid hill conditions of Himachal Pradesh. International Workshop on Integrated Beekeeping Development in South Asia countries, 13-14 Nov 2006, New Delhi, 30

  • Thakur RK (2010) Bumble bee rearing technology in India: present scenario and future strategies. In: National Symposium on Perspectives and Challenges of Integrated Pest Management for Sustainable Agriculture, November 19-21 at Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan

  • Van den Einjde J, De Ruitjer A, Van der Steen J (1991) Method for rearing Bombus terrestris continuously and the production of bumblebee colonies for pollination purposes. Acta Hortic 288:154–158

    Google Scholar 

  • Velthuis HHW, Doorn VVA (2006) A century of advances in bumble bee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie 37:421–451

    Google Scholar 

  • Wilcke J (1953) Het kweden van hommelsvoor de bestuiving van rode klaver. Verslag Van Het Centraal Instituut Voor Landbouwkundig Onderzoek Te Wageningen 43–47

  • Williams PH, Cameron SA, Hines HM, Cederberg B, Rasmont P (2008) A simplified sub generic classification of the bumblebees (Genus Bombus). Apidologie 39:46–74

    Google Scholar 

  • Yankit P (2016) Studies on bumble bee pollination in tomato (Solanum lycopercicum Mill.) under protected condition. M.Sc. Thesis. Department of Entomology, Dr YSP University of Horticulture and Forestry, Nauni, Solan, 68

  • Yeninar H, Duchateau MJ, Kaftanoglu O, Velthuis HHV (2000) Colony developmental patterns in different local populations of Turkish bumblebee Bombus terrestris dalmatinus. J Apic Res 39:107–116. https://doi.org/10.1080/00218839.2000.11101030

    Article  Google Scholar 

  • Yoon HJ, Kim SE, Lee SB, Park IG, Kim NJ, Seong JH, Soo GW (2004) Comparison of the colony development between cardboard and plastic rearing box in the bumblebees, Bombus ignitus. Korean J Apic 19:37–42

    Google Scholar 

  • Yoon HJ, Lee KY (2014) A combination method of CO2-narcosis and cold treatment for breaking diapause of Bombus ignitus and Bombus terrestris bumblebee queens. Int J Ind Entomol 28:58–65

    Google Scholar 

  • Yoon HJ, Lee KY, Kim MA, Ahn MY, Park IG (2013) Optimal cold temperature for the artificial hibernation of Bombus ignitus queen bumblebees. Int J Ind Entomol 26:124–130

    Google Scholar 

  • Zapletal F (1962) Uber die domestikation der hummeln. ArchGeflugelz Kleintierk 10:256–262

    Google Scholar 

Download references

Acknowledgments

We express thanks to All India Coordinated Research Project on Honey bees and Pollinators, Himachal Pradesh Horticulture Development Project and Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan Himachal Pradesh for their assistance in conducting research and preparation of this article.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Diksha Devi.

Ethics declarations

Conflict of interest

There is no conflict of interest between authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharma, H.K., Devi, D., Thakur, M. et al. “Bumble bees” invitro rearing methods – a review. Int J Trop Insect Sci 42, 1045–1057 (2022). https://doi.org/10.1007/s42690-021-00675-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42690-021-00675-5

Keywords

Navigation