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Acaricidal activity of Annona squamosa L. seeds extracts against cattle tick, Rhipicephalus microplus

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Abstract

In the present study, the crude aqueous and chloroform extracts of Annona squamosa was tested for its in-vitro acaricidal efficacy against Rhipicephalus microplus ticks. Concentrations of 2.5%, 5%, 7.5%, 10% and 12.5% were tested against adult ticks whereas 0.312%, 0.625%, 1.25% and 2.5% were tested against larvae. No significant mortality and effect on oviposition was observed using aqueous extract, however, chloroform extract showed strong activity against both viz. adults and larvae. The highest adult mortality was observed at 12.5% concentration and determined LC50 and LC90 values were 5.24% and 14.12% respectively. A dose dependent reduction in egg laying capacity of treated female was observed in all concentrations compared to control group. The recorded mean mortality of larvae was 13.45 ± 0.73, 71.04 ± 1.10, 98.28 ± 0.39 and 100 ± 0.00 observed at 0, 0.312%, 0.625%, 1.25% and 2.5% of concentrations respectively.

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Acknowledgements

The authors are grateful to Associate Dean, College of Veterinary and Animal Sciences, Parbhani, Maharashtra Animal and Fishery Sciences University, for providing necessary facilities to carry out the research work.

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Correspondence to Nitin D. Jadhav or Sachin Kumar.

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Jadhav, N.D., Rajurkar, S.R., Vijay, M. et al. Acaricidal activity of Annona squamosa L. seeds extracts against cattle tick, Rhipicephalus microplus. Int J Trop Insect Sci 42, 1147–1151 (2022). https://doi.org/10.1007/s42690-021-00630-4

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