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Anti-larval activity of actinobacterial extract for Helicoverpa armigera and Spodoptera litura

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Abstract

Gram pod borer (H. armigera) and tobacco caterpillar (S. litura) causes huge economical losses in different crops, identifying a novel and safe biological origin insecticides for their management is always being an important approach. Actinobacteria has significance for their bioactive compounds production and diverse ecological functions. However, little was known for antilarval traits of actinobacteria inhabiting upper gangetic agro-climatic zone of India, so, a study was conducted to explore, isolate and characterize the antilarval actinobacteria. Morphological characters and 16S rRNA gene sequence revealed that isolated actinobacteria strains were belonged to Streptomyces spp., Amycolatopsis spp., Nocardiopsis spp., Saccharopolyspora spp. genera, of which Streptomyces spp. was dominant genera. Streptomyces puniceus IIPR:TB01:01 and Streptomyces badius IIPR:KR04:01 strains (EtoAc extracts) exhibited 78% and 81% larval mortality against H. armigera and S. litura, respectively, were at par with insecticides (Emamectin benzoate 5G and Spinosad 45SC) treatments, indicating those are potential insecticidal strains. Juvenometric activity (extended larval duration and larval-pupal intermediates) of actinobacteria extract was noticed in H. armigera (up to 25 days) and S. litura (up to 28 days) compared to control (15–16 days). Potential actinobacteria strains also witnessed neurotoxic effect (blackening of larva) and lower survival probabilities in H. armigera and S. litura larvae. This study identified phenomena like neurotoxicity and juvenile hormone activity of actinobacterial extract against H. armigera and S. litura. The actinobacteria strains isolated in this study were new and their antilatval potential was not studied earlier, thus current findings advance the isolation and purification of antilarval compounds from aforesaid potential strains.

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Acknowledgements

Authors thankful to the Indian Council of Agricultural Research—Indian Institute of Pulses Research, India (No. CRSCIIPRSIL201600900133) for financial support and also Mr. Ravi Shankar Singh for his help during experiment period.

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Authors and Affiliations

  1. ICAR- Indian Institute of Pulses Research, Kanpur, U.P, India

    Kiran Gandhi Bapatla, Bansa Singh & N. P. Singh

  2. Pandit Deendayal, Upadhayay College of Horticulture & Forestry, Dr. RPCAU, Pusa, Bihar, India

    Krishan Kumar

  3. ICAR-Indian Institute of Farming Systems Research, Modipram, U.P, India

    Lalit Kumar

Authors
  1. Kiran Gandhi Bapatla
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  2. Krishan Kumar
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  3. Lalit Kumar
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  4. Bansa Singh
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  5. N. P. Singh
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Contributions

BKG conceived and designed the concept. BKG and KK maintained the insect cultures and conducted the bioassay experiments. LK and BKG contributed for preparation of actinobacteria extracts. BKG analysed the data. BKG wrote the manuscript. KK, LK, BS and NPS edited the manuscript. KK, LK, BS and NPS contributed for consumables and instruments. All authors approved the manuscript.

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Correspondence to Kiran Gandhi Bapatla.

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Bapatla, K.G., Kumar, K., Kumar, L. et al. Anti-larval activity of actinobacterial extract for Helicoverpa armigera and Spodoptera litura. Int J Trop Insect Sci 42, 495–505 (2022). https://doi.org/10.1007/s42690-021-00565-w

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  • DOI: https://doi.org/10.1007/s42690-021-00565-w

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