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Increased probing activities of green peach aphid (GPA), Myzus persicae, on chitosan-treated caisim (Brassica juncea) monitored by electrical penetration graph (EPG)

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Abstract

Green peach aphid (GPA), Myzus persicae, is a major pest of most horticulture crops. Chitosan-based pest management emerges as an alternative to pesticides due to its biocompatibility and biodegradability properties. Population growth and electrical penetration graph (EPG)-based feeding behavior studies were conducted to assess the effect of chitosan application on caisim against GPA at three concentrations (0.1%, 0.5%, and 1%) including two controls of water and acetic acid. Evident GPA population growth reduction was observed in the chitosan-treated caisim. The effect of chitosan was further monitored by 10 h of EPG recording, which revealed a significant increase in probing activities due to frequent stylet withdrawal that generated high levels of short probing activities. Additionally, inter- and intracellular stylet punctures (waveform C and potential drop-Pd, respectively) displayed a significant increase. However, once the stylet reached the phloem tissue, GPA under chitosan treatment and water control can access the phloem tissue equally, either in terms of the number and duration. Therefore, we suggest that the reduced population growth due to chitosan treatment was related to extra energy consumption during frequent stylet withdrawal and intracellular puncture. This finding indicates the role of chitosan as a plant defense elicitor. However, further investigation regarding this topic is required.

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Acknowledgments

We gratefully thank the support from Applied entomology Laboratory, of Plant Protection Department, Faculty of Agriculture, Universitas Gadjah Mada, and Economic Entomology Research Unit, King Saud University for accessing and utilizing necessary facilities related to EPG monitoring. This study is part of under graduate thesis for Varsha Salsabillah.

Funding

This study was supported by Faculty of Agriculture research funding. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Komplek Bulaksumur, Depok, Sleman, Daerah Istimewa Yogyakarta, 55281, Indonesia

    Varsha Salsabillah, Nugroho Susetya Putra & Alan Soffan

  2. Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P. O. Box 2460, Riyadh, 11451, Kingdom of Saudi Arabia

    Abdulrahman Saad Aldawood

Authors
  1. Varsha Salsabillah
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  2. Nugroho Susetya Putra
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  3. Abdulrahman Saad Aldawood
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  4. Alan Soffan
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Correspondence to Alan Soffan.

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Salsabillah, V., Putra, N.S., Aldawood, A.S. et al. Increased probing activities of green peach aphid (GPA), Myzus persicae, on chitosan-treated caisim (Brassica juncea) monitored by electrical penetration graph (EPG). Int J Trop Insect Sci 41, 2805–2810 (2021). https://doi.org/10.1007/s42690-021-00461-3

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  • DOI: https://doi.org/10.1007/s42690-021-00461-3

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