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Resistance to temephos in Anopheles stephensi larvae is associated with increased cytochrome P450 and α-esterase genes overexpression

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Abstract

The widespread use of synthetic insecticides has resulted in the development of insecticide resistance among disease mosquito vector. Resistance development involves several genes, including CYP450 and esterase family genes. In the current study, expression profiles of seven CYP450 genes namely, CYP9M10, CYP9J40, CYP6AA7, CYP9J34, CYTP450 B9, CYP6M2, CYP6Z2 and three esterase genes namely, esterase β1, alpha esterase, KM234962 were investigated in temephos resistant and susceptible in larvae. The third instar larvae of An. stephensi were subjected to insecticide selection with temephos (Tem Res) following the WHO protocol. Our results show CYP6M2,CYP6Z2 and alpha esterase genes are prominent in temephos resistant in larvae. We suggest that P450 genes and esterase genes, α-esterase and esterase β1 are mostly involved in temephos resistance in larval stage. These results suggest that metabolic resistance may be more prevalent in larval mosquito and play an important role in temephos resistance.

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Acknowledgements

We also thank the Department of Zoology, Periyar University, Salem, Tamil Nadu, India, for providing the infrastructural facility for carrying out this research.

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Authors and Affiliations

  1. Department of Biotechnology, AVS Arts and Science College, Omalur, Tamil Nadu, India

    P. Vivekanandhan

  2. Department of Zoology, Periyar University, Salem, Tamil Nadu, India

    A. Thendralmanikandan

  3. Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, Arusha, Tanzania

    E. J. Kweka & A. M. Mahande

  4. Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania

    E. J. Kweka

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  1. P. Vivekanandhan
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Correspondence to P. Vivekanandhan.

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The present research does not have any evaluation/test in humans performed by any of the authors contains this article. We followed all national, international guidelines for the use of target and non-target organisms were followed.

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Vivekanandhan, P., Thendralmanikandan, A., Kweka, E.J. et al. Resistance to temephos in Anopheles stephensi larvae is associated with increased cytochrome P450 and α-esterase genes overexpression. Int J Trop Insect Sci 41, 2543–2548 (2021). https://doi.org/10.1007/s42690-021-00434-6

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  • DOI: https://doi.org/10.1007/s42690-021-00434-6

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