Abstract
The present study aims to explore the larvicidal activity of n-hexane extract isolated fractions from Artemisia scoparia against third instar larvae of Culex quinquefasciatus mosquito. Twelve fractions, F1 to F12 were separated through column chromatography by using different eluent solvents such as hexane, hexane-chloroform, chloroform and chloroform-methanol, in the order of increasing polarity. Larval bioassay was done by using WHO standard guideline. Larvicidal potential of the separated fractions was evaluated at tested concentrations of 30, 60, 90 and 120 ppm. Mortality rate was scored 24 h post exposure. The fractions F3 and F4 were found more active, exhibiting LC50 values of 28.66 and 22.37 ppm and LC90 values of 53.59 and 53.16 ppm respectively. The larvicidal activity of different fractions was compared by using ANOVA analysis.
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Akhtar N, Rashid A, Murad W, Bergmeier E (2013) Diversity and use of ethno-medicinal plants in the region of Swat, North Pakistan. J Ethnobiol Ethnomed 9:25. https://doi.org/10.1186/1746-4269-9-25
Arivoli S, Raveen R, Samuel T (2015) Larvicidal activity of Citrullus colocynthis (L.) Schrad (Cucurbitaceae) isolated fractions against Aedes aegypti (L.), Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae). Indian J Appl Res 5:97–101
Arivoli S, Raveen R, Samuel T (2015) Larvicidal activity of Murraya koenigii (L.) Spreng (Rutaceae) hexane leaf extract isolated fractions against Aedes aegypti Linnaeus, Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae). J Mosq Res 5:1–8. https://doi.org/10.5376/jmr.2015.05.0018
Baranitharan M, Dhanasekaran S, Kovendan K, Murugan K, Gokulakrishnan J, Benelli G (2017) Coleus aromaticus leaf extract fractions: A source of novel ovicides, larvicides and repellents against Anopheles, Aedes and Culex mosquito vectors? Process Saf Environ Prot 106:23–33. https://doi.org/10.1016/j.psep.2016.12.003
Bockarie MJ, Pedersen EM, White GB, Michael E (2009) Role of vector control in the global program to eliminate lymphatic filariasis. Annu Rev Entomol 54:469–487. https://doi.org/10.1146/annurev.ento.54.110807.090626
Chintem DGW, Nzelibe HC, James DB, Albaba SU, Grillo HT (2014) Larvicidal potential of leaf extracts and purified fraction of Datura stramonium against Culex quinquefasciatus mosquitoes. Int J Nat Sci Res 2:284–293
Geng C-A, Huang X-Y, Chen X-L, Ma Y-B, Rong G-Q, Zhao Y, Zhang X-M, Chen J-J (2015) Three new anti-HBV active constituents from the traditional Chinese herb of Yin-Chen (Artemisia scoparia). J Ethnopharmacol 176:109–117. https://doi.org/10.1016/j.jep.2015.10.032
Ghosh A, Chowdhury N, Chandra G (2012) Plant extracts as potential mosquito larvicides. Indian J Med Res 135:581–598
Habib M, Waheed I (2013) Evaluation of anti-nociceptive, anti-inflammatory and antipyretic activities of Artemisia scoparia hydromethanolic extract. J Ethnopharmacol 145:18–24. https://doi.org/10.1016/j.jep.2012.10.022
Hayes EB, Gubler DJ (2006) West Nile virus: Epidemiology and clinical features of an emerging epidemic in the United States. Annu Rev Med 57:181–194. https://doi.org/10.1146/annurev.med.57.121304.131418
Hematpoor A, Liew SY, Chong WL, Azirun MS, Lee VS, Awang K (2016) Inhibition and larvicidal activity of phenylpropanoids from Piper sarmentosum on acetylcholinesterase against mosquito vectors and their binding mode of interaction. PLoS One 11:e0155265. https://doi.org/10.1371/journal.pone.0155265
Hoang TC, Rand GM (2015) Mosquito control insecticides: A probabilistic ecological risk assessment on drift exposures of naled, dichlorvos (naled metabolite) and permethrin to adult butterflies. Sci Total Environ 502:252–265. https://doi.org/10.1016/j.scitotenv.2014.09.027
Hopp MJ, Foley JA (2001) Global-scale relationships between climate and the dengue fever vector, Aedes Aegypti. Clim Change 48:441–463. https://doi.org/10.1023/a:1010717502442
Keesing F, Belden LK, Daszak P, Dobson A, Harvell CD, Holt RD, Hudson P, Jolles A, Jones KE, Mitchell CE, Myers SS, Bogich T, Ostfeld RS (2010) Impacts of biodiversity on the emergence and transmission of infectious diseases. Nature 468:647–652. https://doi.org/10.1038/nature09575
Khanna VG, Kannabiran K, Rajakumar G, Rahuman AA, Santhoshkumar T (2011) Biolarvicidal compound gymnemagenol isolated from leaf extract of miracle fruit plant, Gymnema sylvestre (Retz) Schult against malaria and filariasis vectors. Parasitol Res 109:1373–1386. https://doi.org/10.1007/s00436-011-2384-6
Mudalungu CM, Matasyoh JC, Vulule JM, Chepkorir R (2013) Larvicidal compounds from Fagaropsis angolensis leaves against malaria vector (Anopheles gambiae). Int J Malar Res Rev 1:1–6
Pandey V, Sharma G, Shankar V, Agrawal V (2013) In vitro bio-control of malarial and filarial vectors using crude extract and isolated fractions of medicinal herbs (Spilanthes spp.) and characterization of the larvicidal compounds. ISHS Acta Hortic 972:87–95. https://doi.org/10.17660/ActaHortic.2013.972.11
Patz JA, Graczyk TK, Geller N, Vittor AY (2000) Effects of environmental change on emerging parasitic diseases. Int J Parasitol 30:1395–1405. https://doi.org/10.1016/S0020-7519(00)00141-7
Ramaiah KD, Ramu K, Kumar KNV, Guyatt H (1996) Epidemiology of acute filarial episodes caused by Wuchereria bancrofti infection in two rural villages in Tamil Nadu, south India. Trans R Soc Trop Med Hyg 90:639–643. https://doi.org/10.1016/S0035-9203(96)90415-0
Saxena RC, Harshan V, Saxena A, Sukumaran P, Sharma MC, Kumar ML (1993) Larvicidal and chemosterilant activity of Annona squamosa alkaloids against Anopheles stephensi. J Am Mosq Control Assoc 9:84–87
Singh HP, Kaur S, Mittal S, Batish DR, Kohli RK, Kit (2008) Phytotoxicity of major constituents of the volatile oil from leaves of Artemisia scoparia Waldst. Z Naturforsch C 63:663–666. https://doi.org/10.1515/znc-2008-9-1009
Singh HP, Kaur S, Mittal S, Batish DR, Kohli RK (2010) In vitro screening of essential oil from young and mature leaves of Artemisia scoparia compared to its major constituents for free radical scavenging activity. Food Chem Toxicol 48:1040–1044. https://doi.org/10.1016/j.fct.2010.01.017
Vidhya PT, Mathew N (2014) Bioassay guided fractionation of Sphaeranthus indicus extract against mosquito vectors. Int J Pharm Sci Res 5:3965–3971
Wahyuni D (2015) New bioinsecticide granules toxin from ectract of papaya (Carica papaya) seed and leaf modified against Aedes aegypti larvae. Procedia Environ Sci 23:323–328. https://doi.org/10.1016/j.proenv.2015.01.047
Wang ZQ, Zhang XH, Yu Y, Tipton RC, Raskin I, Ribnicky D, Johnson W, Cefalu WT (2013) Artemisia scoparia extract attenuates non-alcoholic fatty liver disease in diet-induced obesity mice by enhancing hepatic insulin and AMPK signaling independently of FGF21 pathway. Metabolism 62:1239–1249. https://doi.org/10.1016/j.metabol.2013.03.004
WHO (1996) Report of the WHO informal consultation on the evaluation and testing of insecticides. World Health Organization, Geneva
Acknowledgements
The authors are grateful to Dr. Manzoor Ahmad, Associate Professor and the then Chairperson, Department of Chemistry, University of Malakand, Chakdara, Dir Lower, Pakistan, for his guidance on column chromatography during this study.
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Gul, M., Zahid, M. & Ali, H. Larvicidal potential of different chromatographic fractions of the n-hexane extract of Artemisia scoparia against the vector mosquito Culex quinquefasciatus. Int J Trop Insect Sci 41, 897–902 (2021). https://doi.org/10.1007/s42690-020-00250-4
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DOI: https://doi.org/10.1007/s42690-020-00250-4