Skip to main content

Advertisement

Log in

Pathogenicity of ENTOMOPATHOGENIC nematodes against cabbage butterfly (PIERIS BRASSICAE) LINNAEUS (LEPIDOPTERA: PIERIDAE) in laboratory conditions

  • Original Research Article
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

Cabbage butterfly (Pieris brassicae) is major pest of cabbage. More than 50% yield losses have been attributed due to this pest annually. Seven insect parasitic nematodes i.e. Steinerma carpocapsae, S. feltiae, S. Pakitananese, S. asiaticum, S. glaseri, Heterorhabditis bacteriophora and H. indica were evaluated on 4th larval instar of P. brassicae at the different concentrations. Two best performing EPNs (S. glaseri and H. bacteriophora) were selected and evaluated against 2nd, 3rd and 4th larval instars of Pieris brassicae at 1500 infective juveniles/ml. H. bacteriophora spp. and S. glaseri spp. were sprayed at 1500 IJs/ml on the cabbage leaves alone or in combination (S. glaseri + H. bacteriophora) and 2nd, 3rd and 4th larval instars were fed on them. Among seven EPNs, hundred percent mortality of P. brassicae was recorded in the case of H. bacteriophora and S. glaseri at1500 IJs/ml concentration after 48 h. Both EPNs spp. were found highly effective against all larval instars of P. brassicae. After 48 h of exposure of both EPNs spp. on all larval instar, 100% mortality was recorded. The combined application of H. bacteriophora + S. glaseri on cabbage leaves resulted into 100% mortality of all larval instars of P. brassicae. From present study, it may be concluded that H. bacteriophora and S. glaseri at 1500 IJs/ml concentration found very effective against P. brassicae in laboratory conditions and may be used in the field conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Abdolmaleki A, Maafi Z, Dastjerdi H, Naseri B, Ghasemi A (2017a) Immune defense of Pieris brassicae larvae in challenged with Heterorhabditis bacteriophora, its symbiotic bacteria and metabolites. Invert Surviv J 14:73–84. https://doi.org/10.25431/1824-307X/isj.v14i1.73-84

    Article  Google Scholar 

  • Abdolmaleki A, Rafiee Dastjerdi H, Tanha Maafi Z, Naseri B (2017b) Virulence of two entomopathogenic nematodes through their interaction with Beauveria bassiana and Bacillus thuringiensis against Pieris brassicae (Lepidoptera: Pieridae). J Crop Prot 6:287–299

    Google Scholar 

  • Ali A, Rizvi PQ (2007) Developmental response of cabbage butterfly, Pieris brassicae L.(Lepidoptera: Pieridae) on different cole crops under laboratory and field condition. Asian J Plant Sci 6:1241–1245

    Article  Google Scholar 

  • Anjum MA (2018). The pesticides registered with recommendations for safe handling and use in Pakistan. Pakistan Agricultural Research Council, Ministry of National Food Security and Research, Islamabad

  • Ashfaq F, Butt MS, Nazir A, Jamil A (2018). Compositional analysis of Pakistani green and red cabbage Pak J Agric Sci 55

  • Chaudhuri N, Ghosh S, Ghosh J, Senapati S (2001) Incidence of insect pests of cabbage in relation to prevailing climatic conditions of Terai region. Ind J Entomol 63:421–428

    Google Scholar 

  • Dunphy GB, Bourchier RS (1992) Responses of nonimmune larvae of the gypsy moth, Lymantria dispar, to bacteria and the influence of tannic acid. J Invertebr Pathol 60:26–32. https://doi.org/10.1016/0022-2011(92)90149-X

    Article  CAS  Google Scholar 

  • Forset S, Clarke D (2002). Bacteria-nematode symbiosis. Entomopathogenic nematology. CABI Publishing, United Kingdom

  • Forst S, Dowds B, Boemare N, Stackebrandt E (1997) Xenorhabdus and Photorhabdus spp.: bugs that kill bugs. Annu Rev Microbiol 51:47–72. https://doi.org/10.1146/annurev.micro.51.1.47

    Article  CAS  PubMed  Google Scholar 

  • Gorgadze O, Bakhtadze G, Nebieridze D (2018) Efficacy of local entomopathogenic nematodes against Pieris brassicae (lepidoptera: pieridae). Int J Dev Res 8:23900–23903

    Google Scholar 

  • Khush RS, Lemaitre B (2000) Genes that fight infection: what the Drosophila genome says about animal immunity. Trends Genet 16:442–449. https://doi.org/10.1016/S0168-9525(00)02095-3

    Article  CAS  PubMed  Google Scholar 

  • Krauthausen H-J, Hörner G, Zimmermann S, Voegele R, Brändle F (2018) Competence of Xanthomonas campestris from cruciferous weeds and wallflower (Erysimum cheiri) to induce black rot in cabbage. Eur J Plant Pathol 151:275–289. https://doi.org/10.1007/s10658-017-1371-x

    Article  Google Scholar 

  • Krishnamoorthy A (2004). Biological control of diamondback moth Plutella xylostella (L.), an Indian scenario with reference to past and future strategies. In: Proceedings of the International Symposium (Kirk, A. A and Bordat, D. eds.), Montpellier, France, CIRAD. pp. 204–211

  • Kular JS, Kumar S (2017) Quantification of avoidable yield losses in oilseed Brassica caused by insect pests. J Plant Prot Res 51:38–43

    Article  Google Scholar 

  • Mazurkiewicz A, Tumialis D, Pezowicz E, Skrzecz I, Błażejczyk G (2017) Sensitivity of Pieris brassicae, P. napi and P. rapae (Lepidoptera: Pieridae) larvae to native strains of Steinernema feltiae (Filipjev, 1934). Journal of Plant Diseases and Protection 124:521–524. https://doi.org/10.1007/s41348-017-0118-4

    Article  Google Scholar 

  • Meena R, Ameta O, Meena B (2013) Population dynamics of sucking pests and their correlation with weather parameters in chilli, Capsicum annum L. crop. The Bioscan 8:177–180

    Google Scholar 

  • Pal R, Prasad C (2012) Efficacy of Entomopathogenic nematode, Heterorhabditis indica (Meerut strain) against Lepidopteran insect Pest of agriculture importance. Biosciences 5:321–325

    Google Scholar 

  • Poinar GO (1979) Nematodes for biological control of insects. CRC press, Boca Raton

    Google Scholar 

  • Poinar Jr GO (1990). Taxonomy and biology of Steinernematidae and Heterorhabditidae. Entomopathogenic nematodes in biological control 54

  • Saeed M, Shoukat RF, Zafar J (2017) Population dynamics of natural enemies and insect pest in different Brassica oleracea (cabbage) growing seasons with different production systems. J Entomol Zool Stud 5:1669–1674

    Google Scholar 

  • Sharma R, Singha B, Choudhury SR, Sharma G (2018) Isolation and microscopic investigation of Entomopathogenic nematodes (EPNs) occurring in Barak Valley, Assam, India. Int J Curr Microbiol App Sci 7:1835–1839

    Article  CAS  Google Scholar 

  • Uddin M et al (2007) IPM approach for controlling two lepidopteran pests of cabbage in Bangladesh. Bangl J Entomol 17:19–29

    Google Scholar 

  • Ullah M, Arshad M, Ali S, Iftikhar Y, Mahmood S (2016) Effect of thiamethoxam and some botanical extracts on Cotesia glomerata L.(Braconidae: hymenoptera): an endoparasitoid of Pieris brassicae (L.)(Pieridae: Lepidoptera). Egypt J biol. Pest Control 26:545

    Google Scholar 

  • White G (1927) A method for obtaining infective nematode larvae from cultures. Science (Washington) 66:302–303

    Article  CAS  Google Scholar 

  • Wiesner A (1993). Die Induktion der Immunabwehr eines Insekts (Galleria mellonella, Lepidoptera) durch synthetische Materialien und arteigene haemolymphfaktoren. Verlag nicht ermittelbar

  • Zolfagharian M, Saeedizadeh A, Abbasipour H (2016) Efficacy of two entomopathogenic nematode species as potential biocontrol agents against the diamondback moth, Plutella xylostella (L.). Biol Control 30:78–83. https://doi.org/10.1016/j.tifs.2007.01.004

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Waseem Abbas.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abbas, W., Javed, N., Haq, I.U. et al. Pathogenicity of ENTOMOPATHOGENIC nematodes against cabbage butterfly (PIERIS BRASSICAE) LINNAEUS (LEPIDOPTERA: PIERIDAE) in laboratory conditions. Int J Trop Insect Sci 41, 525–531 (2021). https://doi.org/10.1007/s42690-020-00236-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42690-020-00236-2

Keywords

Navigation