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Bioactivity of essential oils of Cymbopogon citratus (DC) Stapf and Cymbopogon nardus (L.) W. Watson from Benin against Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) infesting yam chips

International Journal of Tropical Insect Science volume 41, pages 511–524 (2021)Cite this article

Abstract

Dinoderus porcellus L. (Coleoptera: Bostrichidae) is a most abundant and damageable pest of stored yam chips in West Africa. In view of the negative effects related to the use of synthetic chemical insecticide, it is important to develop alternative eco-friendly methods of control, such as the use of plant essential oils. The chemical composition and biological effects (repellent, antifeedant, contact and fumigant toxicity) of essential oils from Cymbopogon citratus (DC) Stapf and Cymbopogon nardus (L.) W. Watson were evaluated for the first time against D. porcellus. The chemical composition of C. citratus essential oil revealed that neral (24.64%), geranial (23.46%), and beta-pinene (21.90%) were the predominant compounds whereas citronellal (37.87%), nerol (19.88%) and citronellol (9.11%) were identified in C. nardus essential oil. Data showed that both essential oils were more effective as repellents and fumigant than the commercial insecticide Actellic 50 EC. The results also revealed the low contact toxicity of C. citratus and C. nardus essential oils against D. porcellus. However, similarly to Actellic 50 EC, C. citratus essential oil presented good antifeedant activity against D. porcellus and reduced yam chips weight loss. The results obtained suggest the good potential of C. citratus as both antifeedant and fumigant toxic agent against D. porcellus. While, C. nardus essential oil could be recommended as repellent. However, further studies are required to evaluate the insecticidal activity of both Cymbopogon essential oils under farmers’ storage conditions and to develop a good formulation as biopesticide.

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References

  • Abbott WS (1925) A method for computing the effectiveness of an insecticide. J. Econ. Entomol. 18:265-267

  • Addinsoft (2019) XLSTAT statistical and data analysis solution. Paris, France, https://www.xlstat.com

  • Adepoju OT, Boyejo O, Adeniji PO (2018) Effects of processing methods on nutrient and antinutrient composition of yellow yam (Dioscorea cayenensis) products. Food Chem 238:160–165

    CAS  PubMed  Google Scholar 

  • Agba S, Ode I, Ugbem C, Nwafor S (2019) Impact of yam postharvest activities on standard of living of yam farming households in North-East zone of Benue state Nigeria. J Appl Life Sci Int 21:1–9

    Google Scholar 

  • Ahmad F, Iqbal N, Zaka SM, Qureshi MK, Saeed Q, Khan KA, Ghramhe HA, Ansari MJ, Jaleel W, Aasim M, Awar MB (2018) Comparative insecticidal activity of different plant materials from six common plant species against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) Saudi J Biol Sci. DOI:101016/jsjbs201802018

  • Alcala-Orozco M, Caballero-Gallardo K, Stashenko EE, Olivero-Verbel J (2019) Repellent and Fumigant Actions of the Essential Oils from Elettaria cardamomum (L) Maton Salvia officinalis (L) Linnaeus and Lippia origanoides (V) Kunth against Tribolium castaneum and Ulomoides dermestoides. J Essent Oil-Bear Plants 22:1–13

    Google Scholar 

  • Alves MdeS, Campos IM, Brito DdeMC, Cardoso CM, Pontes EG, Souza MAA (2019) Efficacy of lemongrass essential oil and citral in controlling Callosobruchus maculatus (Coleoptera: Chrysomelidae) a post-harvest cowpea insect pest. Crop Prot 119:191–196

    CAS  Google Scholar 

  • Arasu MV, Al-Dhabi NA, Saritha V, Duraipandiyan V, Muthukumar C, Kim SJ (2013) Antifeedant larvicidal and growth inhibitory bioactivities of novel polyketide metabolite isolated from Streptomyces sp ap-123 against Helicoverpa armigera and Spodoptera litura. BMC Microbiol 13:105

    CAS  PubMed  PubMed Central  Google Scholar 

  • Arivoli S, Tennyson S (2013) Antifeedant activity developmental indices and morphogenetic variations of plant extracts against Spodoptera litura (Fab) (Lepidoptera: Noctuidae). J Entomol Zool Stud 1:87–96

    Google Scholar 

  • Baldacchino F, Tramut C, Salem A, Liѐnard E, Delѐtrѐ E, Franc M, Martin T, Duvallet G, Jay-Robert P (2013) The repellency of lemongrass oil against stable flies tested using video tracking. Parasite 20:1–7

    Google Scholar 

  • Bossou AD, Ahoussi E, Ruysbergh E, Adams A, Smagghe G, De Kimpe N, Avlessi F, Sohounhloue DCK, Mangelinckx S (2015) Characterization of volatile compounds from three Cymbopogon species and Eucalyptus citriodora from Benin and their insecticidal activities against Tribolium castaneum. Ind Crops Prod 76:306–317

    CAS  Google Scholar 

  • Bossou AD, Mangelinckx S, Yedomonhan H, Boko MP, Akogbeto MC, De Kimpe N, Avlessi F, Sohounhloue DCK (2013) Chemical composition and insecticidal activity of plant essential oils from Benin against Anopheles gambiae (Giles). Parasites Vectors 6:337

    PubMed  PubMed Central  Google Scholar 

  • Caballero-Gallardo K, Olivero-Verbel J, Stashenko EE (2012) Repellency and toxicity of essential oils from Cymbopogon martinii Cymbopogon flexuosus and Lippia origanoides cultivated in Colombia against Tribolium castaneum. J Stored Prod Res 50:62–65

    CAS  Google Scholar 

  • Costa AV, Pinheiro PF, Rondelli VM, De Queiroz VT, Tuler CA, Brito KB, Stingue P, Pratisso D (2013) Cymbopogon citratus (Poaceae) essential oil on Frankliniella schultzei (Thysanoptera: Thripidae) and Myzus persicae (Hemiptera: Aphididae). Biosci J Uberlândia 29:1840–1847

    Google Scholar 

  • Descamps LR, Chopa CS, Ferrero AA (2011) Activity of Schinus areira (Anacardiaceae) essential oils against the grain storage pest Tribolium castaneum. Nat Prod Commun 6:887–891

    CAS  PubMed  Google Scholar 

  • Doumbia M, Yoboue K, Kouamé LK, Coffi K, Kra DK, Kwadjo KE, Douan BG, Dagnogo M (2014) Toxicity of Cymbopogon nardus (Glumales: Poacea) against four stored food products insect pests. Intl J Farm Alli Sci 3:903–909

    Google Scholar 

  • FAO (2019) FAOSTAT database. Food and Agriculture Organization, Roma. http://www.fao.org/. Accessed 12 Nov 2019

  • Ferraro V, Piccirillo C, Tomlins K, Pintado ME (2016) Cassava (Manihot esculenta Crantz) and yam (Dioscorea spp) crops and their derived foodstuff: Safety security and nutritional value. Crit Rev Food Sci Nutr 56:2714–2727

    CAS  PubMed  Google Scholar 

  • Fouad R, Bousta D, Lalami AEO, Chahdi FO, Amri I, Jamoussi B, Greche H (2015) Chemical Composition and Herbicidal Effects of Essential Oils of Cymbopogon citratus (DC) Stapf, Eucalyptus cladocalyx, Origanum vulgare L and Artemisia absinthium L. cultivated in Morocco. J. Essent. Oil-Bear. Plants 18:112-123

  • França SM, Oliveira JV, Filho ABE, Oliveira CM (2012) Toxicity and repellency of essential oils to Zabrotes subfasciatus (Boheman) (Coleoptera Chrysomelidae Bruchinae) in Phaseolus vulgaris L. Acta Amaz 42:381–386

    Google Scholar 

  • Franz AR, Knaak N, Fiuza LM (2011) Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera Curculionidae). Rev Bras Entomol 55:116–120

    Google Scholar 

  • Ganjewala D (2009) Cymbopogon essential oils: Chemical compositions and bioactivities. Int J Essen Oil Ther 3:56–65

    CAS  Google Scholar 

  • Gusmao NMS, de Oliveira JV, Navarro DMDAF, Dutra KA, da Silva WA, Wanderley MJA (2013) Contact and fumigant toxicity and repellency of Eucalyptus citriodora Hook Eucalyptus staigeriana F Cymbopogon winterianus Jowitt and Foeniculum vulgare Mill essential oils in the management of Callosobruchus maculatus (FABR) (Coleoptera: Chrysomelidae Bruchinae). J Stored Prod Res 54:41–47

    CAS  Google Scholar 

  • Hernandez-Lambraño R, Pajaro-Castro N, Caballero-Gallardo K, Stashenko E, Olivero-Verbel J (2015) Essential oils from plants of the genus Cymbopogon as natural insecticides to control stored product pests. J Stored Prod Res 62:81–83

    Google Scholar 

  • Hu J, Wang W, Dai J, Zhu L (2019) Chemical composition and biological activity against Tribolium castaneum (Coleoptera: Tenebrionidae) of Artemisia brachyloba essential oil. Ind Crops Prod 128:29–37

    CAS  Google Scholar 

  • Isman MB, Koul O, Luczynski A, Kaminski J (1990) Insecticidal and antifeedant bioactivities of neem oil and their relationship to azadirachtin content. J. Agric. Food Chem. 38: 1406-1411

  • Kabera J, Gasogo A, Uwamariya A, Ugirinshuti V, Nyetera P (2011) Insecticidal effects of essential oils of Pelargonium raveolens and Cymbopogon citratus on Sitophilus zeamais (Motsch). Afr J Food Sci 5:366–375

    Google Scholar 

  • Kim S-W, Kang J, Park I-K (2013) Fumigant toxicity of Apiaceae essential oils and their constituents against Sitophilus oryzae and their acetylcholinesterase inhibitory activity. J Asia Pac Entomol 16:443–448

    CAS  Google Scholar 

  • Kpoviessi S, Bero J, Agbani P, Gbaguidi F, Kpadonou-Kpoviessi B, Sinsin B, Acrombessi G, Frédérich M, Moudachirou M, Quetin-Leclercq J (2014) Chemical composition cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four Cymbopogon species from Benin. J Ethnopharmacol 151:652–659

    CAS  PubMed  Google Scholar 

  • Lale NES, Alaga KA (2001) Exploring the insecticidal larvicidal and repellent properties of Piper guineense Schum et Thonn seed oil for the control of rust-red flour beetle Tribolium castaneum (Herbst) in stored pearl millet Pennisetum glaucum (L) R Br. J Plant Dis Prot 108:305–313

    CAS  Google Scholar 

  • Loko YL, Dansi A, Tamo M, Bokonon-Ganta AH, Assogba P, Dansi M, Vodouhè R, Akoegninou A, Sanni A (2013) Storage insects on yam chips and their traditional management in Northern Benin. Sci World J. Article ID: 484536. 10.1155/2013/484536

  • Loko YLE, Gavoedo DM, Toffa J, Orobiyi A, Thomas-Odjo A, Tamò M (2019) Life table of the predator Alloeocranum biannulipes Montrouzier and Signoret (Hemiptera: Reduviidae) and a test of its ability to suppress populations of Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) in stored yam chips. Biol Control 130:60–69

    Google Scholar 

  • Loko YLE, Toffa DMJ, Orobiyi A (2019) Integrated pest management of the yam chip beetle Dinoderus porcellus Lesne (Coleoptera: Bostrichidae): Current status and future. prospects. In: Soundararajan RP (ed) Entomology IntechOpen. 10.5772/intechopen.87926

  • Nattudurai G, Irudayaraj SS, Paulraj MG, Baskar K, Ignacimuthu S (2015) Insecticidal and Repellent Activities of Toddalia asiatica (L) Lam Extracts against three major stored product pests. Entomol Ornithol Herpetol 4:148

    Google Scholar 

  • Nerio LS, Olivero-Verbel J, Stashenko E (2010) Repellent activity of essential oils: A review. Bioresour Technol 101:372–378

    CAS  PubMed  Google Scholar 

  • Nyamador WS, Ketoh GK, Amévoin K, Nuto Y, Koumaglo HK, Glitho IA (2010) Variation in the susceptibility of two Callosobruchus species to essential oils. J Stored Prod Res 46:48–51

    CAS  Google Scholar 

  • Ocheng F, Bwanga F, Joloba M, Softrata A, Azeem M, Pütsep K, Borg-Karlson A-K, Obua C, Gustafsson A (2015) Essential oils from ugandan aromatic medicinal plants: chemical composition and growth inhibitory effects on oral pathogens. Evid Based Complement Alternat Med 2015:1–10

    Google Scholar 

  • Olatoye KK, Arueya GL (2019) Nutrient and phytochemical composition of flour made from selected cultivars of Aerial yam (Dioscorea bulbifera) in Nigeria. J Food Compos Anal. https://doi.org/10.1016/jjfca201812007

  • Olivero-Verbel J, Nerio LS, Stashenko EE (2010) Bioactivity against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) of Cymbopogon citratus and Eucalyptus citriodora essential oils grown in Colombia. Pest Manag Sci 66:664–668

    CAS  PubMed  Google Scholar 

  • Omohimi CI, Piccirillo C, Roriz M, Ferraro V, Vasconcelos MW, Sanni LO, Tomlins K, Pintado MM, Abayomi LA (2017) Study of the proximate and mineral composition of different Nigerian yam chips flakes and flours. J Food Sci Technol 55:42–51

    PubMed  PubMed Central  Google Scholar 

  • Oni MO, Omoniyi AO (2012) Studies on temperature influence on oviposition and development of immature stages of the yam beetle Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) on dried yam species. J Agric Sci 4:213–218

    Google Scholar 

  • Osuji FNC (1980) Observations on beetles attacking dried yams and yam flour from three Nigerian markets. Trop Stored Prod Info 39:35–38

    Google Scholar 

  • Osunde ZD, Orhevba BA (2009) Effects of storage conditions and storage period on nutritional and other qualities of stored yam (Dioscorea spp) tubers. ‎Afr J Food Agric Nutr Dev 9:678–690

    Google Scholar 

  • Otegbayo BO, Oguniyan DJ, Olunlade BA, Oroniran OO, Atobatele OE (2017) Characterizing genotypic variation in biochemical composition anti-nutritional and mineral bioavailability of some Nigerian yam (Dioscorea spp) land races. J Food Sci Technol 55:205–216

    PubMed  PubMed Central  Google Scholar 

  • Othira JO, Onek LA, Deng LA, Omolo EO (2009) Insecticidal potency of Hyptis spicigera preparations against Sitophilus zeamais (l) and Tribolium castaneum (herbst) on stored maize grains. Afr J Agric Res 4:187–192

    Google Scholar 

  • Padin BS, Fusé C, Urrutia MI, Bello GMD (2013) Toxicity and repellency of nine medicinal plants against Tribolium castaneum in stored wheat. Bull Insectol 66:45–49

    Google Scholar 

  • Paranagama PA, Abeysekera KHT, Nugaliyadde L, Abeywickrama KP (2004) Repellency and toxicity of four essential oils to Sitophilus oryzae L (Coleoptera: Curculionidae). J Natn Sci Found Sri Lanka 32:127–138

    CAS  Google Scholar 

  • Pii TB, Wuana RA, Malu SP, Nwafor SC (2019) Survey of common pesticides used in storage of agricultural produce within Makurdi Benue state Nigeria. Asian J Appl Chem Res 3:1–6

    Google Scholar 

  • Pinto ZT, Sánchez FF, Santos AR, Amaral ACF, Ferreira JLP, Escalona-Arranz JC, Queiroz MMdeC (2015) Chemical composition and insecticidal activity of Cymbopogon citratus essential oil from Cuba and Brazil against housefly. Rev Bras Parasitol Vet 24:36–44

    PubMed  Google Scholar 

  • Saljoqi AUR, Afridi MK, Khan SA, Rehman S (2006) Effects of six plants extracts on rice weevil Sitophilus oryzae in the stored wheat grains. J Agric Biol Sci 1:1–5

    Google Scholar 

  • Samarasekera R, Kalhari KS, Weerasinghe IS (2006) Insecticidal activity of essential oils of Ceylon Cinnamomum and Cymbopogon species against Musca domestica. J Essent Oil Res 18:352–354

    CAS  Google Scholar 

  • Setiawati W, Murtiningsih R, Hasyim A (2011) Laboratory and field evaluation of essential oils from Cymbopogon nardus as oviposition deterrent and ovicidal activities against Helicoverpa armigera Hubner on chili pepper. J Agric Sci 12:9–16

    Google Scholar 

  • Sosan MB, Oyekunle JA (2017) Organochlorine pesticide residue levels and potential human health risks in Kolanuts from selected markets in Osun State southwestern Nigeria. Asian J Chem Sci 2:1–11

    Google Scholar 

  • Stefanazzi N, Stadler T, Ferrero A (2011) Composition and toxic repellent and feeding deterrent activity of essential oils against the stored-grain pests Tribolium castaneum (Coleoptera: Tenebrionidae) and Sitophilus oryzae (Coleoptera: Curculionidae). Pest Manag Sci 67:639–646

    CAS  PubMed  Google Scholar 

  • Taghizadeh SA, Nouri-Ganbalani G, Rafiee-Dastjerdi H, Hadian J (2014) Antifeedant activity and toxicity of some plant essential oils to Colorado potato beetle Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Plant Protect Sci 50:207–216

    Google Scholar 

  • Wang X, Hao Q, Chen Y, Jiang S, Yang Q, Li Q (2015) The effect of chemical composition and bioactivity of several essential oils on Tenebrio molitor (Coleoptera: Tenebrionidae). J Insect Sci 15:116

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We express our sincere gratitude to members of the IITA-Benin entomology laboratory for their contributions to the success of this study. We thank anonymous reviewers for their comments on previous versions of the manuscript.

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Authors and Affiliations

  1. Laboratory of Applied Entomology (LEnA), National Higher School of Applied Biosciences and Biotechnologies (ENSBBA), National University of Science, Technology, Engineering and Mathematics (UNSTIM), BP 14, Dassa, Benin

    Yêyinou Laura Estelle Loko, Parfait Kassa, Joelle Toffa & Omédine Koukoui

  2. Medicinal and organic chemistry Laboratory, Faculty of health Sciences, University of Abomey-Calavi, Cotonou, 01BP 188, Benin

    Sédami Medegan Fagla, Coffi Ayidé Ahouansou, Bienvenu Glinma & Fernand Gbaguidi

  3. Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, 01 BP 2009, Benin

    Victorien Dougnon

  4. Regional Institute of Public Health, University of Abomey-Calavi, 01 BP 526, Abomey-Calavi, Benin

    Salako Luc Djogbenou

  5. International Institute of Tropical Agriculture, Cotonou, 08 BP 0932, Benin

    Manuele Tamò

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  1. Yêyinou Laura Estelle Loko
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  2. Sédami Medegan Fagla
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Contributions

LYLE and MFS contributed to the study conception and design. KP, ACA and TJ performed the experiments. LYLE analysed the data and drafted the manuscript. GB, DV, KO, DSL, SMF, TM and GF corrected the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yêyinou Laura Estelle Loko.

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Loko, Y.L.E., Medegan Fagla, S., Kassa, P. et al. Bioactivity of essential oils of Cymbopogon citratus (DC) Stapf and Cymbopogon nardus (L.) W. Watson from Benin against Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) infesting yam chips. Int J Trop Insect Sci 41, 511–524 (2021). https://doi.org/10.1007/s42690-020-00235-3

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Keywords

  • Insecticidal activity
  • Botanical extract
  • Citronella grass
  • Lemongrass
  • Yam chips

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