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Assessment of insecticide resistance in Culex quinquefasciatus Say with first report on the presence of L1014F mutation from northern districts of West Bengal, India

Abstract

Vector-borne diseases pose serious threat to life and socio-economic status of an individual and application of insecticides has always been a prime strategy to control the vectors. However, with frequent use of insecticides the vectors develop resistance towards the same. It is therefore necessary to evaluate the resistance status of vectors against such chemicals for proper management of vector-borne diseases. As such, field populations of Culex quinquefasciatus a vector of lymphatic filariasis were collected 3 districts of West Bengal and mortality percentage was studied against 6 insecticides. Quantitative assay of major detoxifying enzymes was also studied to unveil their association with insecticide resistance and the presence of mutation in the voltage-gated sodium channel (L1014F) was also evaluated. This study showed multiple resistance to all of the insecticides used i.e., deltamethrin, lambdacyhalothrin, permethrin, DDT, propoxur and malathion). The presence of high frequency of L1014F mutant allele and enhanced enzyme activity may indicate their involvement in the development of insecticide resistance.

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Acknowledgements

The authors express sincere thanks to the Head, Department of Zoology, University of North Bengal, for providing laboratory facilities. The authors are also grateful to the Head, Department of Biotechnology, University of North Bengal, for granting permission to access microplate reader.

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Correspondence to Dhiraj Saha.

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Rai, P., Bharati, M. & Saha, D. Assessment of insecticide resistance in Culex quinquefasciatus Say with first report on the presence of L1014F mutation from northern districts of West Bengal, India. Int J Trop Insect Sci 39, 301–309 (2019). https://doi.org/10.1007/s42690-019-00040-7

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Keywords

  • Insecticide resistance
  • Lymphatic filariasis
  • Insecticide detoxifying enzymes
  • Sodium channel
  • kdr