Abstract
Purpose
Natural latex biomembrane has shown high therapeutic potential for the treatment of diabetic ulcers when associated with phototherapy. In this study, physical and chemical alterations in the latex biomembranes were evaluated after exposure to light-emitting diode (LED) irradiation and after contact with NaCl solution.
Methods
There were three experimental groups: natural latex biomembranes (1) in natura; (2) after 35 min of LED light irradiation; and (3) after immersion in NaCl 0.9 g% solution. The samples were analyzed by tensile strength test, thermogravimetry, contact angle wettability, and Fourier-transform infrared spectroscopy.
Results
The biomembranes that were submitted to contact with NaCl aqueous solution or exposure to LED did not undergo impairment of mechanical resistance. The thermogravimetry analysis indicated that increasing temperature led to mass loss in all samples, referring to their decomposition temperatures. In the wettability test, biomembranes exposed to LED showed a significant reduction in the contact angle between 0 and 10 min, indicating an increase in hydrophilicity, suggesting water storage capacity. The Fourier-transform infrared spectrometry assay showed that there was a more significant reduction in vibration intensity after immersion in NaCl solution compared to untreated biomembranes. An increase in the intensity of the entire spectrum of biomembranes exposed to LED was observed in comparison with the other samples, which may be related to photodegradation caused by LED light irradiation.
Conclusion
The biomembranes underwent significant changes in its chemical structure after being subjected to different types of exposure and maintained its elasticity and tensile strength properties, in addition to showing good water retention capacity after exposure to LED. Thus, it maintains many of its properties for application as a tissue-regenerating biomaterial, even when subjected to LED irradiation and a humid environment.
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Data availability
The datasets presented in this study can be found online in open-source repositories.
References
Agostini DLS, Constantino CJL, Job AE. Thermal degradation of both latex and latex cast films forming membranes. J Therm Anal Calorim. 2008;91:703–7. https://doi.org/10.1007/s10973-007-8351-x.
Al-Thani H, Sathian B, El-Menyar A. Assessment of healthcare costs of amputation and prosthesis for upper and lower extremities in a Qatari healthcare institution: a retrospective cohort study. BMJ Open. 2019;9:e024963. https://doi.org/10.1136/bmjopen-2018-024963.
Bokria JG, Schlick S. Spatial effects in the photodegradation of poly(acrylonitrile-butadiene-styrene): a study by ATR-FTIR. Polymer (guildf). 2002;43:3239–46. https://doi.org/10.1016/S0032-3861(02)00152-0.
Borges FA, Filho EDA, Miranda MCR, Dos Santos ML, Herculano RD, Guastaldi AC. Natural rubber latex coated with calcium phosphate for biomedical application. J Biomater Sci Polym Ed. 2015;26:1256–68. https://doi.org/10.1080/09205063.2015.1086945.
Buche MR, Silberstein MN. Chain breaking in the statistical mechanical constitutive theory of polymer networks. J Mech Phys Solids. 2021;156:104593. https://doi.org/10.1016/J.JMPS.2021.104593.
Carvalho FA, Uchina HS, Borges FA, Oyafuso MH, Herculano RD, Gremião MPD, Santos AG. Natural membranes of Hevea brasiliensis latex as delivery system for Casearia sylvestris leaf components. Rev Bras Farmacogn. 2018;28:102–10. https://doi.org/10.1016/j.bjp.2017.10.007.
Chen Y, Liu Y, Xing T, Sun B, Feng Z, Li P, Yang Z, Li S, Chen S. Effects of salt concentration on the structure and properties of composite fiber of carboxymethyl cellulose/N-2-hydroxylpropyl trimethyl ammonium chloride chitosan prepared by polyelectoyte complexation-freeze drying. Int J Biol Macromol. 2020;151:1030–9. https://doi.org/10.1016/J.IJBIOMAC.2019.11.123.
Chun D, Kim S, Kim J, Yang H-J, Kim JH, Cho J, Yi Y, Kim WJ, Won SH. Epidemiology and burden of diabetic foot ulcer and peripheral arterial disease in Korea. J Clin Med. 2019;8:748. https://doi.org/10.3390/jcm8050748.
Cipriani Frade MA, Brum Cursi I, Fortes Andrade F, Coutinho Netto J, Magalhães Barbetta F, Tiraboschi FN. Management of diabetic skin wounds with a natural latex biomembrane. Med Cutan Ibero Lat Am. 2004;32:157–62.
Costa RHR, Cardoso NA, Procópio RJ, Navarro TP, Dardik A, de Loiola Cisneros L. Diabetic foot ulcer carries high amputation and mortality rates, particularly in the presence of advanced age, peripheral artery disease and anemia. Diabetes Metab Syndr Clin Res Rev. 2017;11:S583-7. https://doi.org/10.1016/j.dsx.2017.04.008.
de Nunes GAMA, dos do Reis MC, Rosa MFF, Peixoto LRT, da Rocha AF, de Rosa SRFS. A system for treatment of diabetic foot ulcers using led irradiation and natural latex. Res Biomed Eng. 2016;32:3–13. https://doi.org/10.1590/2446-4740.0744.
Dickinson LE, Gerecht S. Engineered biopolymeric scaffolds for chronic wound healing. Front Physiol. 2016;7. PMid:27547189. https://doi.org/10.3389/FPHYS.2016.00341.
O Drecun A Striolo C Bernardini 2021 Structural and dynamic properties of some aqueous salt solutions Phys Chem Chem Physhttps://doi.org/10.1039/D0CP05331G
Grech V, Calleja N. WASP (Write a Scientific Paper): parametric vs. non-parametric tests. Early Hum Dev. 2018;123:48–9. https://doi.org/10.1016/j.earlhumdev.2018.04.014.
Guerra NB, Sant’ Ana Pegorin G, Boratto MH, de Barros NR, de Oliveira Graeff CF, Herculano RD. Biomedical applications of natural rubber latex from the rubber tree Hevea brasiliensis. Mater Sci Eng C Mater Biol Appl. 2021;126:112126. https://doi.org/10.1016/j.msec.2021.112126.
Ibrahim S, Othman N, Yusof NH. Preparation, characterization and properties of liquid natural rubber with low non-rubber content via photodegradation. Polym Bull. 2020;782(78):559–75. https://doi.org/10.1007/S00289-019-03030-4.
Kalan L, Grice EA. Fungi in the wound microbiome. Adv Wound Care. 2018;7:247–55. https://doi.org/10.1089/wound.2017.0756.
Kanemitsu K, Kunishima H, Imasaka T, Ishikawa S, Harigae H, Yamato S, Hirayama Y, Kaku M. Evaluation of a low-temperature steam and formaldehyde sterilizer. J Hosp Infect. 2003;55:47–52. https://doi.org/10.1016/S0195-6701(03)00189-0.
Kim D, El-Shall H, Dennis D, Morey T. Interaction of PLGA nanoparticles with human blood constituents. Colloids Surfaces B Biointerfaces. 2005;40:83–91. https://doi.org/10.1016/J.COLSURFB.2004.05.007.
Kulkarni A, Dasari H. Current status of methods used in degradation of polymers: a review. MATEC Web Conf. 2018;144:02023. https://doi.org/10.1051/MATECCONF/201814402023.
Li L, Zeng Z, Wang Z, Peng Z, She X, Li S, Zhong J. Effect of oyster shell powder loading on the mechanical and thermal properties of natural rubber/oyster shell composites. Polym Polym Compos. 2017;25:17–23.
Liu F, Zhang L, Zhou JX, Ni N, Wang Y. Theoretical and experimental analysis of dielectric elastomer unimorph cantilever. Appl Mech Mater. 2014;664:23–7. https://doi.org/10.4028/www.scientific.net/AMM.664.23.
López-Delis A, de S. Rodrigues Fleury Rosa S, de Souza PEN, Carneiro MLB, Rosa MFF, Macedo YCL, Veiga-Souza FH, da Rocha AF. Characterization of the cicatrization process in diabetic foot ulcers based on the production of reactive oxygen species. J Diabetes Res. 2018:1–10. https://doi.org/10.1155/2018/4641364
Miranda MCR, Prezotti FG, Borges FA, Barros NR, Cury BSF, Herculano RD, Cilli EM. Porosity effects of natural latex Hevea brasiliensis on release of compounds for biomedical applications. J Biomater Sci Polym Ed. 2017;28(2117):30. https://doi.org/10.1080/09205063.2017.1377024.
Pan JF, Liu NH, Sun H, Xu F. Preparation and characterization of electrospun PLCL/ poloxamer nanofibers and dextran/gelatin hydrogels for skin tissue engineering. PLoS ONE. 2014;9:1–12. https://doi.org/10.1371/journal.pone.0112885.
Patel A, Xue Y, Mukundan S, Rohan LC, Sant V, Stolz DB, Sant S. Cell-instructive graphene-containing nanocomposites induce multinucleated myotube formation. Ann Biomed Eng. 2016;44:2036–48. https://doi.org/10.1007/s10439-016-1586-6.
Pegorin GS, Leiti MN, Antoniassi M, Chagas ALD, Santana LA, Garms BC, Marcelino MY, Herculano RD, Cipriani Frade MA. Physico-chemical characterization and tissue healing changes by Hancornia speciosa Gomes latex biomembrane. J Biomed Mater Res - Part B Appl Biomater. 2021;109(938):48. https://doi.org/10.1002/jbm.b.34758.
Rahmati M, Pennisi CP, Budd E, Mobasheri A, Mozafari M. Biomaterials for regenerative medicine: Historical perspectives and current trends. Adv. Exp. Med. Biol., vol. 1119, Adv Exp Med Biol; 2018, p. 1–19. PMid:30406362. https://doi.org/10.1007/5584_2018_278
Rodríguez-Cabello JC, González de Torre I, Ibañez-Fonseca A, Alonso M. Bioactive scaffolds based on elastin-like materials for wound healing. Adv Drug Deliv Rev. 2018;129:118–33. https://doi.org/10.1016/J.ADDR.2018.03.003.
Rolere S, Liengprayoon S, Vaysse L, Sainte-Beuve J, Bonfils F. Investigating natural rubber composition with Fourier transform infrared (FT-IR) spectroscopy: a rapid and non-destructive method to determine both protein and lipid contents simultaneously. Polym Test. 2015;43:83–93. https://doi.org/10.1016/j.polymertesting.2015.02.011.
Rosa SSRF, Rosa MFF, Marques MP, Guimarães GA, Motta BC, Macedo YCL, Inazawa P, Dominguez A, Macedo FS, Lopes CAP, da Rocha AF. Regeneration of diabetic foot ulcers based on therapy with red LED light and a natural latex biomembrane. Ann Biomed Eng. 2019a;47:1153–64. https://doi.org/10.1007/s10439-019-02220-5.
Rosa S d. SRF, Rosa MFF, Carneiro MLB, Coelho L, Colón D, Reis CA. Diabetes ground control: a novel system for correcting anomalous stride in diabetic patients. Updat. Manag. Foot Ankle Disord., InTech; 2018. https://doi.org/10.5772/intechopen.74040.
Rosa S de SRF, Rosa MFF, Fonseca MAM, Luz GV da S, Avila CFD, Domínguez AGD, Dantas AGD, Richter VB. Evidence in practice of tissue healing with latex biomembrane: integrative review. J Diabetes Res. 2019b;2019:7457295. https://doi.org/10.1155/2019/7457295
Santos Kotake BG, Gonzaga MG, Coutinho-Netto J, Ervolino E, De Figueiredo FAT, Issa JPM. Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1. Biomed Mater. 2018;13. PMid:29053112. https://doi.org/10.1088/1748-605X/aa9504
Topolniak I, Chapel A, Gaume J, Bussiere PO, Chadeyron G, Gardette JL, Therias S. Applications of polymer nanocomposites as encapsulants for solar cells and LEDs: impact of photodegradation on barrier and optical properties. Polym Degrad Stab. 2017;145:52–9. https://doi.org/10.1016/J.POLYMDEGRADSTAB.2017.06.013.
Vibha SP, Kulkarni MM, Kirthinath Ballala AB, Kamath A, Maiya GA. Community based study to assess the prevalence of diabetic foot syndrome and associated risk factors among people with diabetes mellitus. BMC Endocr Disord. 2018;18(1):9. https://doi.org/10.1186/s12902-018-0270-2.
Vitoriano NAM, Mont’ Alverne DGB, Martins MIS, Silva PS, Martins CA, Teixeira HD, Miranda CB, Bezerra LMM, Montenegro RM, Tatmatsu-Rocha JC. Comparative study on laser and LED influence on tissue repair and improvement of neuropathic symptoms during the treatment of diabetic ulcers. Lasers Med Sci. 2019;34(1365):71. https://doi.org/10.1007/s10103-019-02724-5.
Wang DL, Liu H, Yang D, Wang P, Lu Y, Liu Y. Aminocarbonylation of aryl halides to produce primary amides by using NH4HCO3 dually as ammonia surrogate and base. ChemCatChem. 2017;9:4206–11. https://doi.org/10.1002/cctc.201700893.
Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J. Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement. Sci Rep. 2016;6:1–12. https://doi.org/10.1038/srep24596.
Yang W, Xu H, Lan Y, Zhu Q, Liu Y, Huang S, Shi S, Hancharou A, Tang B, Guo R. Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair. Int J Biol Macromol. 2019;130:58–67. https://doi.org/10.1016/j.ijbiomac.2019.02.120.
Zhang T, Xie F, Motuzas J, Bryant P, Kurusingal V, Colwell JM, Laycock B. Early-stage photodegradation of aromatic poly(urethane-urea) elastomers. Polym Degrad Stab. 2018;157:181–98. https://doi.org/10.1016/J.POLYMDEGRADSTAB.2018.09.020.
Zhou Y, Kosugi K, Yamamoto Y, Kawahara S. Effect of non-rubber components on the mechanical properties of natural rubber. Polym Adv Technol. 2017;28:159–65. https://doi.org/10.1002/pat.3870.
Acknowledgements
This research was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Northeastern Biomaterials Evaluation and Development Laboratory (CERTBIO). We also thank all the volunteers who contributed financially to the development of this research, through an online fundraiser.
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This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001.
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Santana, T.F., Oliveira, R.H.d., dos Santos, L.E. et al. Effect of exposure to a light-emitting diode (LED) on the physicochemical characteristics of natural latex biomembranes used to treat diabetic ulcers. Res. Biomed. Eng. 38, 901–911 (2022). https://doi.org/10.1007/s42600-022-00226-y
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DOI: https://doi.org/10.1007/s42600-022-00226-y