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Evaluating the role of endophytic cyanobacterial isolates on growth promotion and N/P status of rice crop


Cyanobacteria have shown potential in promoting the health and growth of rice plants. A study was undertaken during kharif, 2018 to analyze the effect of two endophytic cyanobacterial strains isolated from the selected rice varieties on growth and yield of basmati (PB 1509) vis a vis non- basmati (Pusa 44) rice cultivars. The cyanobacterial strains namely C1 (a potential nitrogen fixer from rice variety IR 64) and C2 (an effective P solubilizer from rice variety Pusa 1612) belonging to Anabaena sp. were used. The field experiment consisted of control (T1) with recommended dose of fertilizer (RDF) along with four different combinations of fertilizer dose and cyanobacterial strains as T2 (RDF + C1); T3 (75% N in RDF + C1); T4 (RDF + C2) and T5 (RDF with 25% P as MRP and 75% P as SSP + C2), was conducted using split plot design with three replications. Treatments consisted of RDF alone (T1) without inoculum and inoculation with strain C1 with RDF (T2) and with 75% of nitrogen in RDF (T3). Strain C2 was used with RDF (T4) and with 25% P as MRP in RDF (T5). At 90 Days after Transplantation (DAT), the treatments T2 and T3 exhibited better response on per plant basis in terms of plant height (83.3 cm; 85.7 cm), dry weight of leaves (4.24 g; 4.79 g) and stem (13.2 g; 10.0 g) in basmati variety, while, treatment T4 showed better effect (75.3 cm) on plant height in non-basmati variety, which showed maximum dry weight of leaves (5.33 g) and stem (10.8 g) under treatment T5. However, number of tillers, panicle and grain weight per plant depicted variable results. Use of the cyanobacterial strains slightly enhanced N (0.5–1.8%) and P (1.3–2.3%) concentrations in plants at different stages of crop growth as well as at harvest stage.

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  1. Abo-Shady AM (2007) Impact of cyanobacterial inoculation in treated soil with sewage sludge on soil fertility and wheat yield. Egypt J Exp Biol 3:23–32

    Google Scholar 

  2. Ahmad I, Singh YV, Shivay YS, Lata N, Pabbi S (2011) Response to inoculation of nitrogen-fixing and phosphate-solubising microorganisms on growth and yield of basmati rice (Oryza sativa L). Ann Agric Res 32:77–82

    Google Scholar 

  3. Cameron HJ, Julian GR (1988) Utilisation of hydroxyapatite by cyanobacteria as their soles source of phosphate and calcium. Plant Soil 109:123–124

    CAS  Article  Google Scholar 

  4. Chittora D, Meena M, Barupal T, Swapnil P, Sharma K (2020) Cyanobacteria as a source of biofertilizers for sustainable agriculture. Biochem Biophys Rep.

    Article  PubMed  PubMed Central  Google Scholar 

  5. De Mule MCZ, De Caire GZ, De Cano MS, Palma RM, Colombo K (1999) Effect of cyanobacterial inoculation and fertilizers on rice seedlings and post-harvest soil structure. Commun Soil Sci Plant Anal 30:97–107

    Article  Google Scholar 

  6. Gangwar B, Singh VK, Ravi SN (2013) Fertilizer best management practices in important cropping systems. Indian J Fertil 9:34–51

    Google Scholar 

  7. Garlapati D, Chandrasekaran M, Devanesan AN, Mathimani T, Arivalagan P (2019) Role of cyanobacteria in agricultural and industrial sectors: an outlook on economically important byproducts. Appl Microbiol Biotechnol 103:4709–4721

    CAS  Article  Google Scholar 

  8. Ghazal FM, Al-Kassas RA, Hanna MM (2003) Cyanobacteria-wheat association and its influence on wheat grains quality, soils microbial community and soil fertility. In: Applied microbiology conference, Cairo, pp 40–52

  9. Gonçalves AL (2021) The use of microalgae and cyanobacteria in the improvement of agricultural practices: a review on their biofertilising, biostimulating and biopesticide roles. Appl Sci 11(1–21):871.

    CAS  Article  Google Scholar 

  10. Grzesik M, Romanowska- DZ, Kalaji HM (2017) Effectiveness of cyanobacteria and green algae in enhancing the photosynthetic performance and growth of willow (Salix viminalis L.) plants under limited synthetic fertilizers application. Photosynthetica 55:510–521

    CAS  Article  Google Scholar 

  11. Hashem MA (2001) Problems and prospects of cyanobacterial bio-fertilizer for rice cultivation. Aust J Plant Physiol 28(9):881–888

    Google Scholar 

  12. Karthikeyan N, Prasanna R, Lata and Kaushik B.D. (2007) Evaluating the potential of plant growth promoting cyanobacteria as inoculants for wheat. Eur J Soil Biol 43:23–30

    CAS  Article  Google Scholar 

  13. Kaushik BD (1987) Laboratory Methods for Blue Green Algae. Associated Publishing Company, New Delhi

    Google Scholar 

  14. Khatun W, Mosleh MdUD, Kabir G (2012) Effect of cyanobacteria on growth and yield of boro rice under different levels of urea. J Life Earth Agric Sci 40:23–29

    Google Scholar 

  15. Maqubela MP, Mnkeni PNS, Issa MO, Pardo MTD, Acqui LP (2009) Nostoc cyanobacterial inoculation in South African agricultural soils enhances soil structure, fertility and maize growth. Plant Soil 315:79–92

    CAS  Article  Google Scholar 

  16. Nayak S, Prasanna R, Pabby A, Dominic TK, Singh PK (2004) Effect of urea and BGA-Azolla bio-fertilizers on nitrogen fixation and chlorophyll accumulation in soil cores from rice fields. Biol Fertil Soils 40:67–72

    CAS  Article  Google Scholar 

  17. Olsen BC, Cole CV, Watenabe FS, Dean LA (1954) Estimation of available phosphorus by extraction with sodium carbonate. USDA Circ Number 939:19

    Google Scholar 

  18. Prasanna R, Jaiswal P, Nayak S, Sood A, Kaushik BD (2009) Cyanobacterial diversity in the rizosphere of rice and its ecological significance. Indian J Microbiol 49:89–97

    CAS  Article  Google Scholar 

  19. Rai AN, Bergman B (2002) Creation of new nitrogen-fixing cyanobacteria associations. (Special issue—biology and environment). Proc R Ir Acad 102:65–68

    Article  Google Scholar 

  20. Rasmussen U, Johansson C (2002) Diversity and specificity in cyanobacterial symbioses. (Special issue—biology and environment). Proc R Ir Acad 102B:53–56

    Article  Google Scholar 

  21. Rodriguez AA, Stella AA, Storni MM, Zulpa G, Zaccaro MC (2006) Effects of cyanobacterial extracelular products and gibberellic acid on salinity tolerance in Oryza sativa L. Saline Syst.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Singh AL, Singh PK (1987) Influence of Azolla management on the growth, yield of rice and soil fertility N and P contents of plants and soil. Plant Soil 102:49–54

    Article  Google Scholar 

  23. Singh JS, Kumar A, Rai AN, Singh DP (2016) Cyanobacteria: a precious bio-resource in agriculture, ecosystem, and environmental sustainability. Front Microbiol 7:Article 529.

  24. Song T, Martensson L, Eriksson T, Zheng W, Rasmussen U (2005) Biodiversity and seasonal variation of the cyanobacterial assemblage in a rice paddy field in Fujian, China. Fed Eur Mater Soc Microbiol Ecol 54:131–140

    CAS  Google Scholar 

  25. Subbiah BV, Asija GL (1956) A rapid procedure for estimation of available nitrogen in soils. Curr Sci 25:259–260

    CAS  Google Scholar 

  26. Subramanian G, Sundaram SS (1986) Induced ammonia release by the nitrogen fixing cyanobacterium Anabaena. FEMS Microbiol Lett 37:151–154

    CAS  Article  Google Scholar 

  27. Suri VK, Puri UK (1994) Blue green algae as a potential biofertilizer for rice. Ann Agric Res 15:502–503

    Google Scholar 

  28. Yandigeri MS, Kashyap S, Yadav AK, Srinavasan R, Pabbi S (2011) Studies on mineral phosphate solubilization by cyanobacteria Westiellopsis and Anabaena. Mikrobiologiia 80(4):552–555

    PubMed  Google Scholar 

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Authors wish to acknowledge financial support and funding from Director, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India to carry out this work at CCUBGA, ICAR-IARI.

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Correspondence to Dolly Wattal Dhar.

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Jaiswal, P., Dhar, D.W., Sharma, N. et al. Evaluating the role of endophytic cyanobacterial isolates on growth promotion and N/P status of rice crop. Vegetos (2021).

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  • Cyanobacteria
  • Plant growth
  • Yield
  • Nitrogen and phosphorus concentration
  • Rice crop