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Assignment of genotypes to populations and assessment of genetic diversity of Tecomella undulata trees of Rajasthan (India) using ISSR markers

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Abstract

Tecomella undulata (Sm.) Seem (Bignoniaceae) is an important timber yielding tree species of arid region. It is one of the most dwindling species of Rajasthan (India) which is represented by its fragmented populations. Its population is drastically reduced due its over-exploitation for wood and medicinal use. Knowledge of the natural genetic variation and population structure is an important step for developing an appropriate conservation strategy for the species. For the present study, population structure of the species was investigated using ISSR markers by taking 120 individuals from 12 populations covering the potential pockets of arid state of Rajasthan. The sampled populations harbored high level of genetic diversity as assessed through ISSR markers (H = 0.1646, I = 0.2790) that was partitioned more within the populations (58%) than in between the populations (42%). The results of STRUCTURE and split decomposition analysis revealed the existence of two admixed genetic pools of the species in Rajasthan. The pairwise Nei’s gene distance among the populations demonstrated a narrow range from 0.0028 to 0.213. The isolation by distance analysis carried out through the Mantel test demonstrated that the species witnessed a weak correlation (r2 = 0.010, P = 0.170) among the genetic and geographic distance. Our results suggest that the narrowly distributed Tecomella undulata populations in Rajasthan demonstrated high genetic diversity within the populations, however overall population structure is weak.

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Abbreviations

STRUCTURE:

A software used to study population structure of the species

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Acknowledgements

The authors are thankful to the Director, Arid Forest Research Institute, Jodhpur (Rajasthan) for providing necessary facilities for the investigation and Indian Council of Forestry Research, Dehradun for financial assistance. The authors are also thankful to Dr. Nasser Mohammed, Scientist, Tropical Forest Research Institute, Jabalpur for their support in statistical analysis.

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  1. Genetics and Tree Improvement Division, Arid Forest Research Institute, New Pali Road, Jodhpur, Rajasthan, 342005, India

    Desha Meena & Tarun Kant

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  1. Desha Meena
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  2. Tarun Kant
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Correspondence to Desha Meena.

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Meena, D., Kant, T. Assignment of genotypes to populations and assessment of genetic diversity of Tecomella undulata trees of Rajasthan (India) using ISSR markers. Vegetos 35, 317–329 (2022). https://doi.org/10.1007/s42535-021-00294-y

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