Skip to main content
SpringerLink
Log in

Identification of resistant sources of mungbean [Vigna radiata (L.) Wilczek] against mungbean yellow mosaic virus (MYMV) by field evaluation and linked molecular markers

  • Research Articles
  • Published:
Vegetos Aims and scope Submit manuscript

Abstract

Vigna radiata (L.) Wilczek, commonly known as mungbean, moong, and green gram is one of the important pulse crops. It has much potential to feed future populations due to high protein and Fe content in its grains. Worldwide, the yield of mungbean is very low and despite of many efforts, it is not much increased yet. Its low yield is due to its susceptibility to biotic stresses among which, mungbean yellow mosaic virus (MYMV) is the most destructive and prevalent. Identification of new sources of MYMV resistance is one of some novel plant traits in focus to increase its yield. To identify the source of resistance against MYMV, 35 mungbean genotypes were screened under field condition for two seasons and linked molecular markers against MYMV resistance. As a result, two genotypes namely, Meha and IPM 0205-7 were highly resistant against MYMV under field condition and also amplified MYMV resistance markers. Hence, these genotypes can be of great use to develop MYMV resistant varieties or populations. MYMV resistance linked molecular markers namely, OPP 07895 and OPS 07900 successfully discriminated highly resistant and highly susceptible mungbean genotypes against MYMV, which can be used for marker assisted selection (MAS) in particular genotypes or populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Availability of data and material

Not applicable.

Code availability

Not applicable.

References

  • Akhtar KP, Haidar S, Khan MKR, Ahmad M, Sarwar N, Murtaza MA, Aslam M (2010) Evaluation of Gossypium species for resistance to cotton leaf curl Burewala virus. Ann Appl Biol 157(1):135–147

    Article  CAS  Google Scholar 

  • Akhtar KP, Sarwar G, Abbas G, Asghar MJ, Sarwar N, Shah TM (2011) Screening of mungbean germplasm against mungbean yellow mosaic India virus and its vector Bemisia tabaci. Crop Prot 30(9):1202–1209

    Article  Google Scholar 

  • Ali S, Khan MA, Zeshan MA, Habib A, Haider MS (2015) Characterization of conducive environmental conditions for mungbean yellow mosaic virus disease incidence on mungbean germplasm. Pak J Phytopathol 27(1):27–30

    Google Scholar 

  • Ammavasai S, Phogat DS, Solanki IS (2004) Inheritance of resistance to mungbean yellow mosaic virus (MYMV) in green gram [Vigna radiata (L.) Wilczek]. Indian J Genet Plant Breed 64(2):146

    Google Scholar 

  • Bashir M (2005) Studies on viral diseases of major pulse crops and identification of resistant sources. Technical annual report (April, 2004 to June, 2005) of ALP Project. In: Crop Sciences Institute, National Agricultural Research Centre, Islamabad, p 169

  • Chaudhari BA, Patel MP, Dharajiya DT, Tiwari KK (2019) Assessment of genetic diversity in castor (Ricinus communis L.) using microsatellite markers. Biosci Biotechnol Res Asia 16(1):61–69

    Article  Google Scholar 

  • Chen HM, Ku HM, Schafleitner R, Bains TS, Kuo CG, Liu CA, Nair RM (2013) The major quantitative trait locus for mungbean yellow mosaic Indian virus resistance is tightly linked in repulsion phase to the major bruchid resistance locus in a cross between mungbean [Vigna radiata (L.) Wilczek] and its wild relative Vigna radiata subsp. Sublobata. Euphytica 192(2):205–216

    Article  Google Scholar 

  • Dharajiya DT, Ravindrababu Y (2019) Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [Vigna radiata (L.) Wilczek]. Vegetos 32(4):532–539

    Article  Google Scholar 

  • Dharajiya DT, Khadia SM, Pagi NK, Khatrani TJ, Jasani HV, Khunt AD, Ravindrababu Y (2017) Modified method of high quality genomic DNA extraction from mungbean [Vigna radiata (L.) Wilczek] suitable for PCR based amplification. Ind J Sci Technol 10(20):1–7

    Article  CAS  Google Scholar 

  • Dharajiya DT, Ravindrababu Y, Pagi NK (2018) Screening of Mungbean [Vigna radiata (L.) Wilczek] genotypes for resistance against mungbean yellow mosaic virus (MYMV) under field condition. Int J Curr Microbiol Appl Sci 7(5):3472–3483

    Article  Google Scholar 

  • Dharajiya DT, Shah A, Galvadiya BP, Patel MP, Srivastava R, Pagi NK, Solanki SD, Parida SK, Tiwari KK (2020) Genome-wide microsatellite markers in castor (Ricinus communis L.): identification, development, characterization, and transferability in Euphorbiaceae. Ind Crops Prod 151:112461

    Article  CAS  Google Scholar 

  • Dhole VJ, Reddy KS (2013) Development of a SCAR marker linked with a MYMV resistance gene in mungbean (Vigna radiata L. Wilczek). Plant Breed 132(1):127–132

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19(1):11–15

    Google Scholar 

  • Duffy S, Holmes EC (2008) Phylogenetic evidence for rapid rates of molecular evolution in the single-stranded DNA begomovirus tomato yellow leaf curl virus. J Virol 82(2):957–965

    Article  CAS  Google Scholar 

  • Gelotar MJ, Dharajiya DT, Solanki SD, Prajapati NN, Tiwari KK (2019) Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers. Bull Natl Res Centre 43(1):1–10

    Article  Google Scholar 

  • Gowda CLL, Chaturvedi SK, Gaur PM, Kumar SCV, Jukanti AK (2015) Pulses research and development strategies for India. In: Pulses handbook 2015. Commodity India, Bangalore, pp 17–33

  • Gupta SK, Souframanien J, Gopalakrishna T (2008) Construction of a genetic linkage map of black gram based on molecular markers and comparative studies. Genome 51:628–637

    Article  CAS  Google Scholar 

  • Kang BC, Yeam I, Jahn MM (2005) Genetics of plant virus resistance. Annu Rev Phytopathol 43:581–621

    Article  CAS  Google Scholar 

  • Karthikeyan A, Sudha M, Pandiyan M, Senthil N, Shobana VG, Nagarajan P (2011) Screening of MYMV resistant mungbean (Vigna radiata L. Wilczek) progenies through agroinoculation. Int J Plant Pathol 2(3):115–125

    Article  Google Scholar 

  • Karthikeyan A, Shobhana VG, Sudha M, Raveendran M, Senthil N, Pandiyan M, Nagarajan P (2014) Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia. Int J Pest Manage 60(4):314–324

    Article  CAS  Google Scholar 

  • Kitsanachandee R, Somta P, Chatchawankanphanich O, Akhtar KP, Shah TM, Nair RM, Bains TS, Sirari A, Kaur L, Srinives P (2013) Detection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean (Vigna radiata (L.) Wilczek) in India and Pakistan. Breed Sci 63(4):367–373

    Article  CAS  Google Scholar 

  • Maiti S, Basak J, Kundagrami S, Kundu A, Pal A (2011) Molecular marker-assisted genotyping of mungbean yellow mosaic India virus resistant germplasms of mungbean and urdbean. Mol Biotechnol 47(2):95–104

    Article  CAS  Google Scholar 

  • Mathivathana MK, Murukarthick J, Karthikeyan A, Jang W, Dhasarathan M, Jagadeeshselvam N, Sudha M, Vanniarajan C, Karthikeyan G, Yang TJ, Senthil N (2019) Detection of QTLs associated with mungbean yellow mosaic virus (MYMV) resistance using the interspecific cross of Vigna radiata× Vigna umbellata. J Appl Genet 60(3):255–268

    Article  CAS  Google Scholar 

  • Mishra IOP, Mukherjee SK, Mohapatra KB (2012) Response of different mungbean (Vigna radiata L.) varieties against whitefly (Bamicia tabaci Genn.) and yellow mosaic virus incidence. J Plant Prot Environ 9(2):78–79

    Google Scholar 

  • Mishra GP, Dikshit HK, Ramesh SV, Tripathi K, Kumar RR, Aski M, Singh A, Roy A, Kumari N, Dasgupta U, Nair RM (2020) Yellow mosaic disease (YMD) of mungbean (Vigna radiata (L.) Wilczek): current status and management opportunities. Front Plant Sci 11:918

    Article  Google Scholar 

  • Nair RM, Pandey AK, War AR, Hanumantharao B, Shwe T, Alam AKMM, Pratap A, Malik SR, Karimi R, Mbeyagala EK, Dauglas CA, Rane J, Schafleitner R (2019) Biotic and abiotic constraints in mungbean production—progress in genetic improvement. Front Plant Sci 10:1340

    Article  Google Scholar 

  • Nariani TK (1960) Yellow mosaic of mung (Phaseolus aureus L.). Ind Phytopathol 13(1):24–29

    Google Scholar 

  • Parita B, Kumar SN, Darshan D, Karen P (2018) Elucidation of genetic diversity among ashwagandha [Withania somnifera (L.) Dunal] genotypes using EST-SSR markers. Res J Biotechnol 13(10):52–59

    CAS  Google Scholar 

  • Paul PC, Biswas MK, Mandal D, Pal P (2013) Studies on host resistance of mungbean against mungbean yellow mosaic virus in the agro-ecological condition of lateritic zone of West bengal. The Bioscan 8(2):583–587

    Google Scholar 

  • Project Coordinators Report-2018 (2018) All India Coordinated Research Project on MULLaRP (Mungbean, Urdbean, Lentil, Lathyrus, Rajmash, Fieldpea) (Kalyanpur, Kanpur: ICAR-Indian Institute of Pulses Research), pp 1–46

  • Reddy KS (2009) A new mutant for yellow mosaic virus resistance in mungbean (Vigna radiata (L.) Wilczek) variety SML-668 by recurrent gamma-ray irradiation. In: Shu QY (ed) Induced plant mutation in the genomics era. Food and Agriculture Organization of the United Nations, Rome, pp 361–362

    Google Scholar 

  • Sandhu TS, Brar JS, Sandhu SS, Verma MM (1985) Inheritance of resistance to mungbean yellow mosaic virus in green gram. J Res PAU 22:607–611

    Google Scholar 

  • Selvi R, Muthiah AR, Manivannan N, Raveendran TS, Manickam A, Samiyappan R (2006) Tagging of RAPD marker for MYMV resistance in mungbean (Vigna radiata (L.) Wilczek). Asian J Plant Sci 5(2):277–280

    Article  CAS  Google Scholar 

  • Shukla GP, Pandya BP (1985) Resistance to yellow mosaic in green gram. SABRAO J Breed Genet 17:165–171

    Google Scholar 

  • Singh CM, Kumar R, Mishra SB, Pandey A, Arya M (2015) Characterization of mungbean genotypes against mungbean yellow mosaic virus and cercospora leaf spot diseases under north east plain zone. Int J Agric Environ Biotechnol 8(1):119–125

    Article  Google Scholar 

  • Singh CM, Singh P, Pratap A, Pandey R, Purwar S, Douglas CA, Baek KH, Mishra AK (2019) Breeding for enhancing Legumovirus resistance in mungbean: current understanding and future directions. Agronomy 9(10):622

    Article  CAS  Google Scholar 

  • Singh CM, Pratap A, Gupta S, Biradar RS, Singh NP (2020) Association mapping for mungbean yellow mosaic India virus resistance in mungbean (Vigna radiata L. Wilczek). 3 Biotech 10(2):1–12

    Google Scholar 

  • Sowmini K, Palaniappan J (2014) Validation of molecular markers linked with yellow mosaic disease resistance in blackgram Vigna mungo (L.) Hepper. Legume Genomics Genet 5(4):25–30

    Google Scholar 

  • Suman S, Sharma VK, Kumar H, Shahi VK (2018) Re-evaluation of the mungbean [Vigna radiata (L.) Wilczek] genotypes for resistance to mungbean yellow mosaic virus (MYMV) under screen-house conditions. Int J Curr Microbiol Appl Sci 7(4):2821–2829

    Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the authorities of Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India, to provide materials and facilities to conduct the research.

Funding

Not applicable.

Author information

Authors and Affiliations

  1. Bio Science Research Centre, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Sardarkrushinagar, 385506, Gujarat, India

    Darshan T. Dharajiya & Kapil K. Tiwari

  2. Department of Plant Molecular Biology and Biotechnology/Department of Genetics and Plant Breeding, C. P. College of Agriculture, SDAU, Sardarkrushinagar, 385506, Gujarat, India

    Darshan T. Dharajiya & Kapil K. Tiwari

  3. Pulses Research Station, SDAU, Sardarkrushinagar, 385506, Gujarat, India

    Y. Ravindrababu

Authors
  1. Darshan T. Dharajiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kapil K. Tiwari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Ravindrababu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

DTD and YR conceptualized and designed the research. DTD performed the field experiment and molecular work. KKT guided and helped in molecular work. YR guided in field screening and helped in analysis of data. DTD wrote the manuscript.

Corresponding author

Correspondence to Darshan T. Dharajiya.

Ethics declarations

Conflict of interest

The authors have no conflict of interest.

Ethics approval

Not applicable.

Consent for publication

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dharajiya, D.T., Tiwari, K.K. & Ravindrababu, Y. Identification of resistant sources of mungbean [Vigna radiata (L.) Wilczek] against mungbean yellow mosaic virus (MYMV) by field evaluation and linked molecular markers. Vegetos 34, 814–821 (2021). https://doi.org/10.1007/s42535-021-00264-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42535-021-00264-4

Keywords

Search

Navigation