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Nitric oxide improved salt stress tolerance by osmolyte accumulation and activation of antioxidant defense system in seedling of B. juncea (L.) Czern

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Abstract

The present manuscript elucidates the cytoprotective role of nitric oxide and its relation with ROS and antioxidants in minimizing the effect of salinity in B. juncea (Indian mustard) which is an important salt tolerant crop of arid and semi arid region. NO alleviates the adverse effect of salt on growth, biomass, relative water content and photosynthesis. At 50 mM NaCl concentration, rapid accumulation of NO was observed that is 2.02 folds higher in roots respectively while in leaves and roots insignificant changes were observed. At higher concentration of salt stress (70–100 mM NaCl), considerable rise in H2O2 content and membrane damage were documented which restrained by the application of 100 µM SNP. NO mitigates the salt stress by regulating the activities of nitrate reductase and other antioxidants. Reduction in NO content was observed in independent treatment of NaCl and SNP in comparison to combined treatment of SNP + NaCl (100 µM SNP + 50 mM NaCl). The different antioxidant enzymes show disparate behaviour with signalling molecule NO. The activity of catalase, guaiacol peroxidase and nitrate reductase were upregulated whereas ascorbate peroxidase was downregulated with NO. The elevated activities of antioxidant and NO accumulation might be responsible for detoxifying the detrimental effect of salinity in B. juncea. The current investigation emphasizes on the potential role of NO in salt stress tolerance by regulating antioxidative defense metabolism, cellular homeostasis and detoxification mechanism in seedlings of B. juncea.

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Abbreviations

NO:

Nitric oxide

SNP:

Sodium nitroprusside

NR:

Nitrate reductase

Chl:

Chlorophyll

NaCl:

Sodium chloride

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

MDA:

Malondialdehyde

BSA:

Bovine serum albumin

HgCl2 :

Mercuric chloride

EDTA:

Ethylene diamine tetraacetic acid

CAT:

Catalase

APX:

Ascorbate peroxidase

GPOX:

Guaiacol peroxidase

H2O2 :

Hydrogen peroxide

O2 − :

Superoxide radical

ROS:

Reactive oxygen species

NADH:

Nicotinamide adenine dinucleotide hydride

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Acknowledgements

Author gratefully acknowledges the University Grant Commission, New Delhi for providing financial assistance in the form of Centre for Advanced Study.

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  1. Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, 342005, India

    Khushboo Khator & Gyan Singh Shekhawat

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  1. Khushboo Khator
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  2. Gyan Singh Shekhawat
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Correspondence to Gyan Singh Shekhawat.

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Khator, K., Shekhawat, G.S. Nitric oxide improved salt stress tolerance by osmolyte accumulation and activation of antioxidant defense system in seedling of B. juncea (L.) Czern. Vegetos 32, 583–592 (2019). https://doi.org/10.1007/s42535-019-00071-y

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