Abstract
An efficient in vitro propagation method is developed for Artocarpus lakoocha Roxb. through direct shoot regeneration from cotyledonary node region of seedlings developed from immature seeds. The immature seeds were germinated on Murashige and Skoog (MS) medium having different concentrations of either 6-benzyl aminopurine (BA) or kinetin or Thidiazuron and subsequently, the cotyledonary node with primary shoot was transferred to MS medium without any growth regulator for shoot multiplication. Maximum (7.23 ± 0.46) shoots regenerated when immature seeds were cultured for 21 days on MS medium having 4.44 µM BA for germination. For rooting, shoots were pulse treated for 48 h with different concentrations of Indole-3-acetic acid (IAA) or Indole-3-butyric acid (IBA) followed by transfer on agar gelled MS basal medium. About 94.45% shoots rooted on pulse treatment with 5.0 µM IBA. Plantlets obtained after rooting were hardened and acclimatized into soil with 85–90% survival. The plantlets established into soil had similar vegetative morphology to the mother plant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chabukswar MM, Deodhar MA (2005) Rooting and hardening of in vitro plantlets of Garcinia indica Chois. Indian J Biotech 4:409–413
Choudhary R, Chaudhury R, Malik SK (2015) Development of an efficient regeneration and rapid clonal multiplication protocol for three different Morus species using dormant buds as explants. J Hortic Sci Biotech 90:245–253
Dhavala A, Rathore TS (2010) Micropropagation of Embelia ribes Burm f. through proliferation of adult plant axillary shoots. Vitro Cell Dev Biol Plant 46:180–191
Giri CC, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and application of biotechnology to trees: an overview. Trees Struct Funct 18:115–135
Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119
Jagtap UB, Bapat VA (2010) Artocarpus: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 129:142–166
Joshee N, Bastola DR, Agrawal VP, Yadav AK (1991) Lakoocha: A multipurpose tree of warm climate. In: Whipkey A (ed) Janick J. Trends in new crops and uses ASHS Press, Alexandria, pp 405–406
Kamal NHM, Aziz MA, Kadzimin S, Rashid AA (2018) In vitro mass multiplication of Artocarpus heterophyllus Lam var. Tekam yellow. Pertanika J Trop Agric Sci 41:1289–1314
Lu YF, Zhang LR, Wang YR, Yao YC (2015) Establishment of an efficient in vitro plantlet regeneration system from leaf explants of ornamental crabapple (Malus spp.). J Hortic Sci Biotech 90:585–592
Moharana A, Das A, Subudhi E, Naik SK, Durga P, Barik DP (2017) High frequency shoot proliferation from cotyledonary node of Lawsonia inermis L. and validation of their molecular finger printing. J Crop Sci Biotech 21:405–416
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Pandey S, Jaiswal VS (2002) Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes. Indian J Expt Biol 10:950–952
Pandey S, Singh M, Jaiswal U, Jaiswal VS (2006) Shoot initiation and multiplication from a mature tree of Terminalia arjuna. In Vitro Cell Dev Biol – Plant 42: 389-393.
Rahman MA, Amin MN (1995) High frequency in vitro plantlets regeneration from an endangered tree- Artocarpus lakoocha Roxb. Plant Tiss Cult 5:137–145
Rani U, Sharma MM, Ismail N, Batra A (2012) In vitro plant regeneration from immature seeds of Murraya koeniggi (L.) Spreng. Indian J Biotech 11:108–110
Sharma PK, Tyagi P, Sharma KC, Kothari SL (2003) Clonal micropropagation of Crateva adansoni (DC) Prodr: a multipurpose tree. Vitro Cell Dev Biol Plant 39:156–160
Singh M, Jaiswal U, Jaiswal VS (2001) Thidiazuron-induced shoot multiplication and plant regeneration in bamboo (Dendrocalamus strictus Nees.). J Plant Biochem Biotech 10:133–137
Singh M, Jaiswal U, Jaiswal VS (2004) In vitro regeneration and improvement in tropical fruit trees: an assessment. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Springer, Dordrecht, pp 228–243
Singh M, Jaiswal VS, Jaiswal U (2014) Thidiazuron-induced anatomical changes and shoot morphogenesis in Dendrocalamus strictus Nees. Can J Pure Appl Sci 8:2901–2904
Snedecor GW, Cochran WG (1989) Statistical methods. Iowa State University Press, Ames
Tripathi M, Kumari N (2010) Micropropagation of tropical fruit tree Spondias mangifera Willd. through direct organogenesis. Acta Physiol Plant 32:1011–1015
Wongkham S, Wongkham C, Boonsiri P, Simasathiansophon S, Trisonthi C, Atisook K (1995) Isolectins from seeds of Artocarpus lakoocha. Phytochem 40:1331–1334
Acknowledgements
Financial assistance vide letter no. F. No. 33-468/2007 (SR) by University Grants Commission, New Delhi, India to MS is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Singh, M., Bhatti, S. & Verma, S.K. Improved plant regeneration method of Artocarpus lakoocha Roxb. from immature seeds. Vegetos 32, 269–274 (2019). https://doi.org/10.1007/s42535-019-00041-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42535-019-00041-4