Abstract
Cultivation of edible mushrooms is one of the most economically usable processes for bioconversion of agro wastes for the production of protein-rich food with various medicinal values. The present investigation was focused to determine the most effective and suitable substrate for the cultivation of Pleurotus ostreatus considering biological efficiency and medicinal activities of fruiting bodies. Different parameters like mycelium running rate, development of fruiting bodies, cropping phase, productivity, biological efficiency and medicinal activities like antibacterial, antioxidant activities of this mushroom were evaluated. The total mycelium running, total primordial formation, complete fruit body formation and duration of cropping were dominant in PS substrate. Fruiting body size was bigger in SD substrate but higher productivity (19.6%) and biological efficiency (65.33%) was observed in PS substrate. Methanolic extracts of cultivated P. ostreatus on PS substrate showed higher antibacterial, free radical scavenging activity (74.26%), chelating effects on ferrous ion (91.11%), total phenol content (0.097 mg GAE/g) and flavonoids content (0.017 mg CE/g). The lowest EC50 values of cultivated P. ostreatus were found in PS substrate which indicated the stronger ability of antioxidant activity. The present study indicates that the types of used substrates affect biological efficiency and medicinal properties.
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Abbreviations
- PS:
-
Paddy straw
- SD:
-
Sawdust
- PT:
-
Time required for complete fruit body formation
- HT:
-
Time required to primordia formation to harvesting
- RT:
-
Time required to complete mycelium running in substrate packets in days
- T:
-
Duration of cropping
- SE:
-
Standard error
- FWM:
-
Mushroom fresh weight
- FWC:
-
Compost fresh weight
- P:
-
Productivity
- BE:
-
Biological efficiency
- DWC:
-
Corresponds to dry weight of compost
- FRS:
-
Free radical scavenging
- DPPH:
-
1,1-Diphenyl-2-picrylhydrazyl
- BHT:
-
Butylated hydroxytoluene
- EDTA:
-
Ethylenediaminetetraacetic acid
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Acknowledgements
The authors are grateful to the Head, Department of Botany, Tripura University for providing all sorts of facilities. The authors are also thankful to Forest Department of Tripura, Government of Tripura, India, for giving the permission of fieldwork in forest areas. The first author is thankful to the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India for the financial assistance received through a Project (Sanctioned Order No. BT/463/NE/TBP/2013).
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SD had done all the experimental work, data recording, substrates collection, preparation of culture and also writing part. RS had done preparation of substrates and also regularly spraying water into an inoculated substrate. PD had done the all statistical analysis. AKS had done the scientific work plan, identification and regularly checked the experimental data. All authors read and approved the final manuscript.
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Debnath, S., Saha, R., Das, P. et al. Cultivation and medicinal properties of wild edible Pleurotus ostreatus of Tripura, Northeast India. Vegetos 32, 238–246 (2019). https://doi.org/10.1007/s42535-019-00035-2
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DOI: https://doi.org/10.1007/s42535-019-00035-2