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Breeding for low-temperature stress tolerance in Hevea brasiliensis: screening of newly developed clones using latex biochemical parameters

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Abstract

Identification of high yielding genotypes with abiotic stress tolerance is one of the most important aspects of crop improvement in Hevea to expand rubber cultivation to non-traditional regions and to meet the needs of the domestic market. This study was aimed to evaluate 20 clones for yield potential and cold stress tolerance and for subsequent selection of cold-tolerant clones suitable for cultivation in cold stress-prone non-traditional regions. Parameters such as girth, dry rubber content (DRC), volume of latex, dry rubber yield, and biochemical parameters like adenosine triphosphate (ATP), sucrose, and thiol contents in latex were estimated in 9-year-old trees grown in cold stress-prone region (Agartala), in comparison with plants grown under congenial climatic conditions in a traditional rubber growing tract. A significant positive correlation was observed between yield with girth, latex volume, DRC, ATP, and sucrose content in latex and an inverse relationship with thiol. Principal component analysis based on growth, yield, physiological, and biochemical parameters revealed six variables contributing primarily to the first component PC 1, thus explaining 54% of the total variance. A clear separation of thiols was observed from the rest of the traits. Six clones such as P 21, P 110, P 107, P 20, P 17, and P 57 were found superior to the check clones RRII 430, RRIM 600, RRII 429, and RRII 105, while clone P 17 exhibited better performance in the traditional region. Further validation of these clones by large-scale evaluation only would indicate the candidate clone for recommendation to the cold stress-prone non-traditional regions of India.

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Abbreviations

DRC:

Dry rubber content

ATP:

Adenosine triphosphate

PC:

Principal component

PCA:

Principal component analysis

RRII:

Rubber research institute of India

RRIM:

Rubber research institute of Malaysia

P:

Pipeline

NR:

Natural rubber

NE:

North-Eastern

RRS:

Regional research station

CES:

Central experimental station

TCA:

Trichloroacetic acid

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Acknowledgements

The authors are indebted to Dr. C. Narayanan, Senior Scientist, RRII for sharing the juvenile yield data and for permitting the sample collection from the clonal nursery and Dr. S. Sreelatha for her suggestions in designing the experiment, critically analysing the data, and reviewing the paper. The help rendered by Dr. K. Annamalainathan, Joint Director of Plant Physiology Division, RRII, Dr. S.K. Dey, Joint Director, RRS, Agartala, Dr. G.C. Mondal, Senior Scientist, Dr. Debabrata Rai Scientist, Mr. Brajesh, Scientific Assistant and Mr. Harikumar, Scientific Assistant are gratefully acknowledged. The weather data provided by Dr. Shammi Raj and Ms. T. Sailajadevi is gratefully acknowledged. The authors also thank Mr. P. Aneesh, Assistant Statistician for helping with the data analysis. Ms. Suparna V. Ajith (Ref. No: 09/662(0022)/2018-EMR-I dated 05/08/2020) and Neethu N. Nair (Ref. No: 09/662(0020)/2017-EMR-I dated 26/06/2020) are grateful to CSIR for the SRF Fellowship.

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  1. Rubber Research Institute of India, Rubber Board, Kottayam, Kerala, 686 009, India

    Suparna V. Ajith, Neethu N. Nair, M. B. Mohamed Sathik & T. Meenakumari

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  1. Suparna V. Ajith
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  2. Neethu N. Nair
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  3. M. B. Mohamed Sathik
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Correspondence to M. B. Mohamed Sathik.

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Ajith, S.V., Nair, N.N., Sathik, M.B.M. et al. Breeding for low-temperature stress tolerance in Hevea brasiliensis: screening of newly developed clones using latex biochemical parameters. J Rubber Res 24, 819–828 (2021). https://doi.org/10.1007/s42464-021-00143-2

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