Abstract
The etiopathogenesis of Behçet’s Disease is not clearly understood. Recurrent aphthous stomatitis is seen in Behçet’s Disease. Mucous membranes are protected by general host defenses, a specific immune response to damage caused by free radicals under normal conditions. Nitric oxide is a free radical that plays a role in chronic and acute inflammation and systemic inflammatory response. Our purpose was to investigate the levels of nitric oxide in serum and saliva samples from patients with Behçet’s Disease and recurrent aphthous stomatitis and from a control group. In the clinical study, 22 patients with Behçet’s Disease, 24 patients with recurrent aphthous stomatitis, and 27 healthy individuals were included. Serum and saliva samples were taken from each group to examine nitric oxide levels. When the nitric oxide levels of serum and saliva were evaluated, the only statistically significant finding was that nitrate and nitrite levels were lower in the control group than in patients with Behçet’s Disease and recurrent aphthous stomatitis. There was no difference between patients with Behçet’s Disease and patients with recurrent aphthous stomatitis. We conclude that nitric oxide levels increase in patients with Behçet’s Disease and recurrent aphthous stomatitis, both in serum and saliva. The results of nitric oxide in saliva and serum were found to be compatible. Saliva can be used to evaluate nitric oxide in Behçet’s Disease and recurrent aphthous stomatitis. Salivary nitric oxide levels may be an important marker for estimating the severity of Behçet’s Disease for clinicians.
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References
Zeidan MJ, Saadoun D, Garrido M, Klatzmann D, Six A, Cacoub P. Behcet’s disease physiopathology: a contemporary review. Auto Immun Highlights. 2016;7:4.
Preeti L, Magesh K, Rajkumar K, Karthik R. Recurrent aphthous stomatitis. J Oral Maxillofac Pathol. 2011;15:252–6.
Lewkowicz N, Lewkowicz P, Kurnatowska A, Banasik M, Glowacka E, Cedzynski M, et al. Innate immune system is implicated in recurrent aphthous ulcer pathogenesis. J Oral Pathol Med. 2003;32:475–81.
Gunduz K, Ozturk G, Sozmen EY. Erythrocyte superoxide dismutase, catalase activities and plasma nitrite and nitrate levels in patients with Behcet disease and recurrent aphthous stomatitis. Clin Exp Dermatol. 2004;29:176–9.
Halliwell B, Cross CE. Oxygen-derived species: their relation to human disease and environmental stress. Environ Health Perspect. 1994;102:5–12.
Babior BM. Phagocytes and oxidative stress. Am J Med. 2000;109:33–44.
Evereklioglu C, Turkoz Y, Er H, Inaloz HS, Ozbek E, Cekmen M. Increased nitric oxide production in patients with Behcet’s disease: is it a new activity marker? J Am Acad Dermatol. 2002;46(1):50–4.
International Study Group for Behçet’s disease, criteria for diagnosis of Behcet’s disease. Lancet 1990; 335: 1078–1080.
O'Duffy JD. Vasculitis in Behcet's disease. Rheum Dis Clin N Am. 1990;16:423–31.
Orem A, Vanizor B, Cimşit G, et al. Decreased nitric oxide production in patients with Behcet’s disease. Dermatology. 1999;198:33–6.
Tabak LA. A revolution in biomedical assessment: the development of salivary diagnostics. J Dent Educ. 2001;65:1335–9.
Saral Y, Coskun BK, Ozturk P, Karatas F, Ayar A. Assessment of salivary and serum antioxidant vitamins and lipid peroxidation in patients with recurrent aphthous ulceration. Tohoku J Exp Med. 2005;206:305–12.
Piskin S, Sayan C, Durukan N, Senol M. Serum iron, ferritin, folic acid, and vitamin B12 levels in recurrent aphthous stomatitis. J Eur Acad Dermatol Venereol. 2002;16:66–7.
Volk T, Ioannidis I, Hensel M, Degroot H, Kox WJ. Endothelial damage induced by nitric oxide: synergism with reactive oxygen species. Biochem Biophys Res Commun. 1995;213:196–203.
Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology. and pharmacology Pharmacol Rev. 1991;43:109–42.
Stuehr DJ, Griffith OW. Mammalian nitric oxide synthases. Adv Enzymol Relat Areas Mol Biol. 1992;65:287–346.
McCartan BE, Lamey PJ, Wallace AM. Salivary cortisol and anxiety in recurrent aphthous stomatitis. J Oral Pathol Med. 1996;25:357–9.
Kirschbaum C, Klauer T, Filipp SH, Hellhammer DH. Sex-specific effects of social support on cortisol and subjective responses to acute psychological stress. Psychosom Med. 1995;57:23–31.
Tsuchiya T, Kishimoto J, Koyama J, et al. Modulatory effect of L-NAME, a specific nitric oxide synthase (NOS) inhibitor, on stress-induced changes in plasma adrenocorticotropic hormone (ACTH) and corticosterone levels in rats: physiological significance of stress-induced NOS activation in hypothalamic-pituitary-adrenal axis. Brain Res. 1997;776:68–74.
Anggard E. Nitric oxide: mediator, murderer and medicine. Lancet. 1994;343:1199–206.
Das D, Banerjee RK. Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem. 1993;125:115–25.
Ohashi M, Iwase M, Nagumo M. Elevated production of salivary nitric oxide in oral mucosal diseases. J Oral Pathol Med. 1999;28:355–9.
Djeraba Z, Benlabidi F, Djaballah-Ider FZ, Medjeber O, Arroul-Lammali A, Belguendouz H, et al. Vitamin D status in Algerian Behcet’s disease patients: an immunomodulatory effect on NO pathway. Immunopharmacol Immunotoxicol. 2017;39:243–50.
Sancak B, Onder M, Oztas MO, et al. Nitric oxide levels in Behcet’s disease. J Eur Acad Dermatol Venereol. 2003;17:7–9.
Yildirim M, Baysal V, Inaloz HS, Doguc D. The significance of serum nitric oxide levels in Behcet’s disease and recurrent aphthous stomatitis. J Dermatol. 2004;31:983–8.
Sunitha M, Shanmugam S. Evaluation of salivary nitric oxide levels in oral mucosal diseases: a controlled clinical trial. Indian J Dent Res. 2006;17:117–20.
Jagtap K, Baad RK. Estimation of salivary nitric oxide in recurrent aphthous ulcer and oral lichen planus patients with its clinical significance. J Contemp Dent Pract. 2012;13:623–6.
Yilmaz G, Sizmaz S, Yilmaz ED, et al. Aqueous humor nitric oxide levels in patients with Behcet disease. Retina. 2002;22:330–5.
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Menziletoglu, D., Kucukkolbasi, H., Dursun, R. et al. Evaluatıon of Serum and Salıva Nıtrıc Oxıde Levels ın Recurrent Aphthous Stomatıtıs and Behcet’s Dısease. SN Compr. Clin. Med. 1, 334–338 (2019). https://doi.org/10.1007/s42399-019-0045-8
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DOI: https://doi.org/10.1007/s42399-019-0045-8