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Effects of Peritoneal Dialysis Solution on Experimental Necrotizing Enterocolitis in Newborn Rats

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Abstract

Necrotizing enterocolitis (NEC) is one of the most urgent gastrointestinal diseases in premature newborns. We utilized a newborn model of hypoxia and hypothermia that resembles human NEC to investigate the effects of irrigation with peritoneal dialysis solution (PDS) on the treatment of NEC. For the NEC model, 1-day-old rats were stressed with 100% CO2 for 5 min by placing them in an airtight perfused box; then, they were exposed to cold stress in 4 °C for 10 min twice daily for the next 4 days. The newborn rats (n = 24) were divided into four equal groups: group 1 (control), group 2 (NEC), group 3 (NEC + irrigated with saline), and group 4 (NEC + irrigated with PDS). The animals in group 3 were irrigated with intraperitoneal saline, and the animals in group 4 were irrigated with PDS twice daily for the next 2 days. Then, all animals were sacrificed on day 7, and intestinal specimens were harvested. Histopathologic damage of the intestine was evaluated, and there was a highly significant difference between the control group and groups 2 and 3. In group 4, values were significantly less than in the other groups (2 and 3), except the control group. Also, significant differences were found when group 4 was compared with groups 2 and 3 (P < 0.0001). We believe peritoneal irrigation with PDS may be efficacious in NEC patients to obtain better results.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

    Hatice Sonay Yalcin Comert, Haluk Sarihan & Mustafa Imamoglu

  2. Department of Pathology, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

    Ismail Saygin

  3. Department of Pediatric Surgery, Trabzon Numune Hospital, Trabzon, Turkey

    Dilek Basar

  4. Department of Public Health, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

    Gamze Can

Authors
  1. Hatice Sonay Yalcin Comert
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  2. Haluk Sarihan
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  3. Ismail Saygin
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  4. Mustafa Imamoglu
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  5. Dilek Basar
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  6. Gamze Can
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Correspondence to Hatice Sonay Yalcin Comert.

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The experimental protocol was approved by the Animal Care and Use Committee of Karadeniz Techniquel University.

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The authors declare that they have no conflict of interest.

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Comert, H.S.Y., Sarihan, H., Saygin, I. et al. Effects of Peritoneal Dialysis Solution on Experimental Necrotizing Enterocolitis in Newborn Rats. SN Compr. Clin. Med. 1, 547–551 (2019). https://doi.org/10.1007/s42399-019-00079-0

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  • DOI: https://doi.org/10.1007/s42399-019-00079-0

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