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Evaluation of cellulase production from Aspergillus niger and Aspergillus heteromorphus under submerged and solid‐state fermentation

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Abstract

The present study aimed at cellulase production from two fungal strains of Aspergillus niger and Aspergillus heteromorphus under solid-state fermentation (SSF) and submerged fermentation (SmF) conditions. The agricultural residue (rice straw) was tested for its suitability as solid support as well as a carbon source for microbial growth. The potential of fungi to produce enzyme rely on the species of the fungus, growth substrate (lignocellulosic biomass) and culturing method. In the present work, rice straw was used as a substrate for fungal growth under SSF and SmF conditions. Maximum cellulase activities as 6.4 IU/g FPase and 125 IU/g CMCase under SSF condition, and 3.8 IU/g FPase and 94 IU/g CMCase under SmF were achieved on the 5th day of incubation from A. heteromorphus. Similarly, FPase and CMCase activity for A. niger under SSF were 5.8 IU/g and 113 IU/g, respectively, while FPase activity 3.5 IU/g and CMCase activity 88 IU/g was observed under SmF condition. A. heteromorphus shows higher cellulase production ability as compared to A. niger, and the SSF condition is better than SmF for enzyme production.

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Funding

This experimental study was carried in Major Research Project granted by UGC (No. F33-144/2007 (SR) date 28.02.08). So, authors AS and NRB are thankful to UGC for its financial assistance.

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Authors and Affiliations

  1. Department of Environmental Science & Engineering, Guru Jambheshwar University of Science & Technology, Hisar, Haryana, 125001, India

    Anita Singh, Somvir Bajar & Narsi R. Bishnoi

  2. Department of Environmental Sciences, Central University of Jammu, Jammu, Jammu and Kashmir, 181143, India

    Anita Singh & Arti Devi

  3. Department of Environmental Sciences, J.C. Bose University of Science & Technology, YMCA, Faridabad, Haryana, 121006, India

    Somvir Bajar

Authors
  1. Anita Singh
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  2. Somvir Bajar
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  4. Narsi R. Bishnoi
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Contributions

NRB and AS designed the outline of the manuscript, AS conducted the experiments in Lab, AS and SB calculated and compiled the data. AS, SB and AD performed the literature research and prepared the manuscript, and NRB and AS critically revised the manuscript.

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Correspondence to Anita Singh.

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Singh, A., Bajar, S., Devi, A. et al. Evaluation of cellulase production from Aspergillus niger and Aspergillus heteromorphus under submerged and solid‐state fermentation. Environmental Sustainability 4, 437–442 (2021). https://doi.org/10.1007/s42398-021-00173-x

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  • DOI: https://doi.org/10.1007/s42398-021-00173-x

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