Abstract
The present study was primarily emphasized on isolation of yeast from diverse samples and screening for potential biosurfactant producer. Yeast isolates AH16, AH23, AH46 and AH62 showed effective oil displacement activity and were confirmed to be potential biosurfactant producers. Among them, maximum yield of 4.23 g/l of crude biosurfactant was extracted from isolate AH62 within 96 h of fermentation which also showed effective emulsification activity of 76.4 ± 0.38% when tested against diesel. It could further decrease the surface tension of water from 72 to 31.4 ± 0.38 mN/m and was hence selected for subsequent study. The isolate was identified as Candida sp. AH62 through phylogenetic analysis. Characterization of extracted biosurfactant using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), gas chromatography (GC) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) revealed sophorolipid to exist as a heterogeneous mixture of lactonic and acidic forms. The isolated sophorolipids showed potential antibacterial activity towards pathogenic Gram negative and Gram positive bacteria.
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Author KA would like to thank UGC-BSR-RFSMS and authors thank DST PURSE (C-DST-PURSE-II/43/2018) for providing financial support to carry out this study.
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Archana, K., Sathi Reddy, K., Parameshwar, J. et al. Isolation and characterization of sophorolipid producing yeast from fruit waste for application as antibacterial agent. Environmental Sustainability 2, 107–115 (2019). https://doi.org/10.1007/s42398-019-00069-x
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DOI: https://doi.org/10.1007/s42398-019-00069-x