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Assessment of Trichoderma spp. from Misiones (Argentina) as biocontrol agents and plant growth-promoting fungi on the basis of cell wall degrading enzymes and indole acetic acid production

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Abstract

The present study aims to characterise the biological control agent (BCA) and plant growth-promoting fungi (PGPF) traits of four Trichoderma strains from Misiones soil. To achieve this, a dual confrontation assay was conducted, and production of β-1,3-glucanase, chitinase, and indole acetic acid (IAA) was quantified. All Trichoderma strains inhibited the growth of yerba mate root pathogens. LBM116 showed the highest β-1,3-glucanase production. LBM112, LBM120, and LBM219 produced the highest chitinase activity. LBM112, LBM116, and LBM120 displayed the highest indole acetic acid production. The BCA and PGPF indexes proved to be valuable tools for selecting LBM120 for the inoculation assay in yerba mate plants. Yerba mate plants treated with LBM120 demonstrated the highest chlorophyll content, dry weight and incidence percentage compared to the control group. Trichoderma strains from Misiones soil are promising BCA and PGPF for improving the development of yerba mate crops.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We are grateful to the Alberto Roth Foundation for providing the yerba-mate plants for the fungal strains and the greenhouse use in the inoculation assays. We thank Edgar Poletto for providing the root pathogens.

Funding

This work was funded by an innovation project “Universidades Agregando Valor 2017” award N°4285-APN-SECPU#ME/17 provided by the Science and Technology Secretary, Misiones National University and, the PICT-START UP 2017-0007 project granted by the National Agency of Scientific and Technological Promotion.

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Authors and Affiliations

  1. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales, Instituto de Biotecnología Misiones “Dra. María Ebe Reca” (INBIOMIS), Laboratorio de Biotecnología Molecular (BIOTECMOL), Ruta Nacional 12 Km 7.5, C.P. 3300, Misiones, Argentina

    Ana Clara López, Anibal Sebastián Chelaliche, José Alejandro Alderete, Pedro Darío Zapata & Adriana Elizabet Alvarenga

  2. CONICET, Buenos Aires, Argentina

    Ana Clara López, Anibal Sebastián Chelaliche, Pedro Darío Zapata & Adriana Elizabet Alvarenga

Authors
  1. Ana Clara López
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  2. Anibal Sebastián Chelaliche
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  3. José Alejandro Alderete
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  4. Pedro Darío Zapata
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  5. Adriana Elizabet Alvarenga
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by ACL. The first draft of the manuscript was written by ACL and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ana Clara López.

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The authors have no relevant financial or non-financial interests to disclose.

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López, A.C., Chelaliche, A.S., Alderete, J.A. et al. Assessment of Trichoderma spp. from Misiones (Argentina) as biocontrol agents and plant growth-promoting fungi on the basis of cell wall degrading enzymes and indole acetic acid production. Indian Phytopathology 76, 923–928 (2023). https://doi.org/10.1007/s42360-023-00658-1

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