Abstract
Wild species related to agricultural crops may increase the adaptive capacity of agricultural systems worldwide. They represent a large pool of genetic diversity for drawing new allelic variation in breeding programs. Crop wild relatives have been an extremely valuable source of novel alleles for economic traits which when transferred to cultivated species adapt well to changing biotic stress, farming practices, market demands, and climatic conditions. The present study was conducted using 79 wild pigeonpea accessions belonging to three distinct genera viz., Cajanus, Rhynchosia, and Flemingia. These were grown in wilt sick field at ICAR-Indian Institute of Pulses Research (IIPR), Kanpur, India during Kharif, 2016–17, 2017–18 and 2018–19 for identifying the durable resistant sources against wilt caused by Fusarium udum (Race-2) for further use in the resistant breeding program. On the basis of pathogenicity and molecular characterization (MW341594), the pathogen was confirmed as F. udum (Race-2) isolated from wilted plants. Among the 35 resistant accessions, 24 belong to the primary gene pool of Cajanus, and 11 were from the tertiary genera Rhynchosia. Eleven accessions were moderately resistant, 14 showed susceptibility, and 19 exhibited highly susceptible reactions against wilt disease.
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References
Bohra A, Mallikarjuna N, Saxena KB, Upadhyaya H, Vales I, Varshney R (2010) Harnessing the potential of crop wild relatives through genomics tools for pigeonpea improvement. J Plant Biol 37(1):83–98
Butler EJ (1906) The wilts disease of pigeonpea and pepper. Agril J India 1:25–36
Cooper HD, Spillane C, Hodgkin T (2001) Broadening the genetic base of crop production. CAB International, Wallingford
Hajjar R, Hodgkin T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156:1–13
Hannes D, Gregory B, Justin A, Benjamin K, Chelsea S, Guarino L (2017) Past and future use of wild relatives in crop breeding. Crop Sci 57:1070–1082
Haware MP, Nene YL, Pundir RPS, Narayana RJ (1992) Screening of world chickpea germplasm for resistance to fusarium wilt. Field Crops Res 30:147–154
Hufford MB, Lubinksy P, Pyhäjärvi T, Devengenzo MT, Ellstrand NC, Ross-Ibarra J (2013) The genomic signature of crop-wild introgression in maize. PLoS Gene 9(5):1003477. https://doi.org/10.1371/journal.pgen.1003477
Kannaiyan J, Nene YL, Reddy MV, Ryan JG, Raju TN (1984) Prevalence of pigeonpea diseases and associated crop losses in Asia, Africa, and the Americas. Trop Pest Management 30:62–71
Kilian B, Martin W, Salamini F (2010) Genetic diversity, evolution and domestication of wheat and barley in the fertile crescent. In: Glaubrecht M (ed) Evolution in action. Springer, Berlin, Heidelberg, pp 137–166
Kumar S, Gupta S, Chandra S, Singh BB. (2004) How wide is genetic base of pulse crops? In: Ali M, Singh BB, Kumar S, Dhar V (Eds), Pulses in new perspective: Proceedings of the National Symposium on Crop Diversification and Natural Resources Management, Kanpur, India 20–22 Dec. 2003. Indian Soc of Pulses Res and Dev, Kanpur, p 211–221
Leslie JF, Summerell BA (2006a) The fusarium laboratory manual. Blackwell Publishing, Oxford, UK
Leslie JF, Summerell BA (2006b) The Fusarium laboratory manual, 1st edn. Blackwell Publishing Ltd, Oxford, London
Mallikarjuna N, Moss JP (1995) Production of hybrids between Cajanus platycarpus and Cajanus cajan. Euphytica 83:43–46
Mallikarjuna N, Jadhav D, Reddy MV, Dutta Tawar U (2005) Introgression of phytophthora blight resistance from Cajanus platycarpus into short-duration pigeonpea [Cajanus cajan (L.). Millsp.]. Indian J Genet Plant Breed 65:261–263
Mishra RK, Naimuddin SPR, Satheesh Naik SJ, Bohra A, Singh F, Singh IP, Kumar K (2016) Evaluation of promising lines of pigeonpea for resistance to wilt caused by Fusarium udum Butler. Jr Food Legumes 29(1):64–66
Moore G (2015) Strategic pre-breeding for wheat improvement. Nat Plants 3:15018
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326
Naik Satheesh SJ, Byre Gowda M, Venkatesha SC, Ramappa HK, Pramila CK, Mary Reena GA, Ramesh S (2012) Molecular diversity among pigeonpea genotypes differing in response to pigeonpea sterility mosaic disease. J Food Legumes 25(3):194–199
Nash SM, Snyder WC (1962) Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52:567–572
Nene YL, Haware MP (1980) Screening chickpea for resistance to wilt. Plant Dis 64:379–380
Nene YL, Kannaiyan J, Reddy MV. (1981) Pigeonpea diseases: Resistance-screening techniques. Information bulletin no. 9. International Crops Research Institute for the Semi Arid Tropics, Patancheru 502 324, AP, India
Nene YL, Sheila VK (1990) Pigeonpea: geography and importance. In: Nene YL, Hall SD, Sheila VK (eds) The Pigeonpea. CAB International, Wallingford, Oxon, pp 1–14
Okiror MA (2002) Genetics of resistance to Fusarium udum in pigeonpea [Cajanus cajan (L.) Millsp. Indian J Genet 62:218–220
Pande S, Sharma M, Mangala UN, Ghosh R, Sundaresan G (2011) Phytophthora blight of pigeonpea [Cajanus cajan (L.) Millsp.]: An updating review of biology, pathogenicity and disease management. Crop Prot 30:951–957. https://doi.org/10.1016/j.cropro.2011.03.031
Prescott-Allen C, Prescott-Allen R (1986) The first resource: wild species in the North American economy. Yale Univ. Press, New Haven, CT
Reddy MV, Nene YL, Kannaiyan J, Raju TN, Saka VN, Davor AT, Songa WP, Omanga P (1990) Pigeonpea lines resistant to wilt in Kenya and Malawi. Intl Pigeonpea News Lett 16:34
Reddy MV, Raju TN, Sharma SB, Nene YL, McDonald D, Pande S, Sharma M, (2012) Handbook of Pigeonpea Diseases (Revised). Information Bulletin No. 42. ICRISAT, Patancheru, pp 64
Satheesh Naik SJ, Singh F, Mishra RK, Bohra A, Singh IP, Chaturvedi SK, Singh J, Singh NP (2016) Characterization of genotypes for wilt, quality and agronomic traits in vegetable pigeonpea (Cajanus cajan L. Millisp). J Food Legumes 29(3–4):216–219
Sawler J, Reisch B, Aradhya M, Prins B, Zhong G (2013) Genomics assisted ancestry deconvolution in grape. PLoS One 8(11):80791
Saxena KB, Kumar RV, Rao PV (2002) Pigeonpea nutrition and its improvement. J Crop Prod 5:227–260
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res 22:4673–4680
Van der Maesen LJG. (2006) Cajanus cajan (L.) Millsp. In: Brink M and Belay G (eds) Plant Resources of Africa 1. Cereals and Pulses. Wageninge: Backhuys Publishers. http://database.prota.org/search.ht
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The authors greatly acknowledge the Indian Council of Agricultural Research (ICAR), New Delhi, India for financial support and Director, ICAR-IIPR for continuous support and encouragement.
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Mishra, R.K., Mishra, M., Naik, S.J.S. et al. Identification of resistance sources against Fusarium udum (Race-2) in wild accessions of pigeon pea for strengthening the pre-breeding program. Indian Phytopathology 75, 1197–1203 (2022). https://doi.org/10.1007/s42360-022-00560-2
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DOI: https://doi.org/10.1007/s42360-022-00560-2