Biochemical and histological characterisation of Fusarium oxysporum infected wheat (Triticum aestivum) seedlings in vitro

Banerjee, Arpita; Mittra, Bhabatosh; Das, Anath Bandhu

doi:10.1007/s42360-021-00458-5

Short Communication
Published: 18 January 2022

Biochemical and histological characterisation of Fusarium oxysporum infected wheat (Triticum aestivum) seedlings in vitro

Arpita Banerjee¹,
Bhabatosh Mittra² &
Anath Bandhu Das³

Indian Phytopathology volume 75, pages 559–563 (2022)Cite this article

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Abstract

In the present study response of wheat seedlings to F. oxysporum was studied which revealed that the fungus was successful in establishing infection by suppressing the antioxidative machinery of the plant as evidenced by high and rapid disease incidence. Reactive oxygen species (ROS) accumulation enhanced gradually in infected seedlings with time which was concomitant with the increase in hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) content. Biochemical changes in the activity of catalase (CAT), peroxidase (POX) and glutathione reductase (GR) was used as stress markers. Excepting for POX all the enzymes showed increased activity in the leaves as compared to the roots. Electron microscopic (SEM) analysis revealed damage to the tissue structure showing exposed epidermal layer with loosely arrange cells in infected seedlings. The results presented in this paper add to the knowledge of the events and mechanisms related to the mode of infection of F. oxysporum in wheat.

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Abbreviations

CAT:: Catalase
POX:: Peroxidase
GR:: Glutathione reductase
GST:: Glutathione S-transferase
H₂O₂ :: Hydrogen peroxide
ROS:: Reactive oxygen species
MDA:: Malondialdehyde
SEM:: Scanning electron microscope
HR:: Hypersensitive response
GSH:: Glutathione
dpi:: Days post inoculation
hpi:: Hours post inoculation
TAC:: Total antioxidant capacity
RT:: Room temperature

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Authors and Affiliations

Department of Botany, F M University, Balasore, Odisha, India
Arpita Banerjee
Department of Biosciences and Biotechnology, F M University, Balasore, Odisha, India
Bhabatosh Mittra
Department of Botany, Utkal University, Cuttack, Odisha, India
Anath Bandhu Das

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Arpita Banerjee
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Bhabatosh Mittra
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Anath Bandhu Das
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Banerjee, A., Mittra, B. & Das, A.B. Biochemical and histological characterisation of Fusarium oxysporum infected wheat (Triticum aestivum) seedlings in vitro. Indian Phytopathology 75, 559–563 (2022). https://doi.org/10.1007/s42360-021-00458-5

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Received: 05 January 2021
Revised: 08 December 2021
Accepted: 28 December 2021
Published: 18 January 2022
Issue Date: June 2022
DOI: https://doi.org/10.1007/s42360-021-00458-5

Keywords

Fusarium oxysporum
Triticum aestivum
Antioxidants
Scanning electron microscope
Fungal infection