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Biocontrol prospective of Bacillus siamensis-AMU03 against Soil-borne fungal pathogens of potato tubers

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Abstract

Twelve bacterial strains (GJ01 to GJ12), isolated from an oil-contaminated soil, were characterized through different biochemical, morphological, and molecular technique and analyzed by different screening methods to choose the best biosurfactant producing strain. Only GJ-01 strain exhibited significant biosurfactant activity, while the other strains exhibited partial activity. Molecular identification through 16S  rRNA technique identified GJ-01 as a Bacillus siamensis-AMU03. The effect of the cultural filtrate tested under in vitro study against Rhizoctonia solani and Fusarium oxysporum, with the highest reduction of mycelial growth in dual culture method (89.40% and 90.20% respectively). Both in food poisoning method as well as pathogenicity test, the bioagent was able to inhibit the growth of both the pathogens successfully at 3% concentration, and that was mainly due to volatile metabolites such as surfactin, fengycin and iturin, which were identified and analysed through Liquid chromatography-mass spectrometry. These findings indicated that AMU03 strain had a promising eco-friendly and bio-safe approach to cope against soil-borne and post-harvest pathogens of seed tubers.

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Acknowledgements

The first author is highly thankful to SERB-Dept. of Science and Technology, New Delhi, Govt. of India, New Delhi, for providing the financial grants received under File No. DST-SERB-NPDF/2016/001409 and Dept. of Botany, Aligarh Muslim University, Aligarh, U.P for providing all the facilities/infrastructure, SAIF center for LC-MS-ESI, at CSIR-CDRI, Lucknow.

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Science and Engineering Research Board, PDF/2016/001409, Touseef Hussain.

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  1. Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India

    Touseef Hussain & Abrar Ahmad Khan

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  1. Touseef Hussain
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TH: received the grant, conducted the study, write the draft, editing, AAK: guided and examine the findings.

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Hussain, T., Khan, A.A. Biocontrol prospective of Bacillus siamensis-AMU03 against Soil-borne fungal pathogens of potato tubers. Indian Phytopathology 75, 179–189 (2022). https://doi.org/10.1007/s42360-021-00447-8

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