Abstract
In this experiment an effort was made to synthesize silver nanoparticle biologically from Trichoderma asperellum, a potential indigenous biocontrol agent. Silver nitrate was added as precursor for the synthesis of silver nanoparticle. The biosynthesized silver nanoparticle was characterized by UV–Vis spectrophotometer, dynamic light scattering (DLS), X-ray diffraction (XRD), zeta potential and transmission electron microscope (TEM). UV Vis spectrum of aqueous medium containing silver ion showed peak at a wavelength of 420 nm corresponding Plasmon Absortion of silver nanoparticle. DLS study showed that the biosynthesized silver nanoparticles have a size of 27.64 nm with polydispersity index (PDI) of 0.409. The charge of silver nanoparticle was determined by zeta sizer and found to have negative potential value of − 1.34. It indicates that the biosynthesized nanoparticle are stable on dispersion. TEM study revealed the formation well dispersed silver nanoparticle in the range of 9–41 nm with roughly spherical in shape. Fungicidal activity of silver nanoparticle at different concentration (100 ppm, 50 ppm, 30 ppm, and 10 ppm) tested against four soil borne plant pathogens viz., Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, and Sclerotium rolfsii and comparison was made with Carbendazim @3000 ppm. The result showed that the silver nanoparticle at 100 ppm showed significantly higher efficacy in inhibiting mycelial growth of the pathogens as compared to the Carbendazim at 3000 ppm.
Similar content being viewed by others
References
Al Abboud MA (2018) Fungal biosynthesis of silver nanoparticle and their role in control of fusarium wilt of sweet pepper and soil-borne fungi in vitro. Int J Pharmacol 14(6):773–780
Devi PT, Kulanthaivel S, Kamil D, Borah LJ, Prabhakaran N, Srinivasa N (2013) Biosynthesis of silver nanoparticle from Trichoderma species. Ind J Expt Bio 51:543–547
Elamawi RMA, El-Shafey RAE (2013) Inhibition effects of silver nanoparticle against rice blast disease caused by Magnaporthe grisea. Egypt J Agric Res 91(4):98–105
Kim SW, Jung JH, Lamsal K, Kim YS, Min JS, Lee YS (2012) Antifungal effects of silver nanoparticle (AgNPs) against various plant pathogenic fungi. Korean Soc Mycol Mycobiol 40(53):58
Ruiz-Romero P, Valdez-Salas B, González-Mendoza D, Mendez-Trujillo V (2018) Antifungal effects of silver phytonanoparticle from Yucca shilerifera against strawberry soil-borne pathogens: Fusarium solani and Macrophomina phaseolina. Mycobiology 46(1):47–51
Salvioni L, Galbiati E, Collico V, Alessio G, Avvakumova S, Corsi F, Colombo M (2017) Negatively charged silver nanoparticle with potent antibacterial activity and reduced toxicity for pharmaceutical preparations. Int J Nanomed 12:2517–2530
Tripathi MR, Gupta KR, Shrivastav A, Singh PA, Shrivastav RV, Singh P (2013) Trichoderma koningii assisted biogenic synthesis of silver nanoparticle and evaluation of their antibacterial activity. Adv in Nat Sci: Nanosci and Nanotech 4(3):32–35
Zhang X, He X, Wang K, Wang Y, Li H, Tan W (2009) Biosynthesis of size-controlled gold nanoparticle using fungus, Penicillium sp. J Nanosci Nanotechnol 10:5738–5744
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Kaman, P.K., Dutta, P. Synthesis, characterization and antifungal activity of biosynthesized silver nanoparticle. Indian Phytopathology 72, 79–88 (2019). https://doi.org/10.1007/s42360-018-0081-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42360-018-0081-4