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Indian Phytopathology

, Volume 71, Issue 3, pp 445–452 | Cite as

Molecular investigations of gene expression analysis in two contrasting genotypes of banana during fusarium wilt (Foc1) infection

  • Pavitra Kotari
  • Rekha Ajitha
  • Kundapura V. RavishankarEmail author
Short Communication
  • 19 Downloads

Abstract

Fusarium oxysporum f. sp. cubense is a destructive fungal pathogen causing vascular wilt disease (Panama disease) in banana. The present study examines the pattern of expression of 18 defense-related genes, during Foc1 infection, using two contrasting banana genotypes ‘Calcutta-4’ (Resistant) and ‘Kadali’ (susceptible). The results showed that the expression analysis of 18 selected genes in Resistant genotype ‘Calcutta-4’ showed a peak of upregulation at both 3 and 10 days post inoculation. In all, early induction of the 18 defense genes could be used as indicators of the activation of defense responses in banana genotypes to fusarium wilt infection. The analysis of results indicates early up-regulation of genes involved in signalling and in antioxidants expression, and later, genes involved in cell wall modification and synthesis of secondary metabolites which impart tolerance to Foc1.

Keywords

Banana FocHost–pathogen interaction Pathogenesis Fusarium wilt Defense genes 

Notes

Acknowledgements

Authors thank the Indian Council of Agricultural Research, New Delhi for financial assistance through the ICAR Network Project on Transgenics in Crops: Functional Genomics-Fusarium wilt and drought tolerance in Banana.

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Copyright information

© Indian Phytopathological Society 2018

Authors and Affiliations

  • Pavitra Kotari
    • 1
    • 2
  • Rekha Ajitha
    • 3
  • Kundapura V. Ravishankar
    • 1
    Email author
  1. 1.Division of BiotechnologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  2. 2.Department of Biotechnology, Centre for Post Graduate StudiesJain UniversityBengaluruIndia
  3. 3.Division of Fruit CropsICAR-Indian Institute of Horticultural ResearchBengaluruIndia

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