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Indian Phytopathology

, Volume 71, Issue 3, pp 337–348 | Cite as

Identification and confirmation of downy mildew (Pseudoperonospora cubensis Berk. & Curt.) resistance sources in cucumber (Cucumis sativus L.)

  • J. C. Bommesh
  • M. PitchaimuthuEmail author
  • A. T. Sadashiva
  • S. Sriram
  • B. Varalakshmi
  • K. V. Ravishankar
Research Article

Abstract

An experiment was designed to identify the resistance source for downy mildew disease in different genotypes of cucumber. Forty-one cucumber genotypes were screened under natural condition and also under artificial epiphytic condition using detached leaf assay method. Genotype IIHR-438 and Cucumis metuliferus L. showed field resistance with an average PDI of 17.66 and 17.46; AUDPC of 772.24 and 764.48, respectively compared to 73.12 PDI and AUDPC of 3096.64 in highly susceptible genotype of IIHR-389. The disease reaction in selected genotypes of cucumber confirmed by artificial screening was in accordance with disease reaction under natural conditions. Resistant genotype IIHR-438 (14.3 PDI) and C. metuliferus L. (12.8 PDI) had least average PDI as compared to susceptible check Swarna Agethi (58.00 PDI) under artificial condition. Statistical analysis of disease severity data over a period for all the forty-one genotypes using non-linear growth model revealed that 99% variability in disease progression. Screening of genotypes under field conditions, sporulation of pathogen, progress of disease, detached leaf assay and non-linear statistical analysis implied that none of genotypes were found to be immune to downy mildew. Wherein the genotype, IIHR-438 and wild cucumber (C. metuliferus L.) were found resistant to downy mildew disease. Hence, it can be utilized in breeding program to develop resistant cultivar in cucumber against Pseudoperonospora cubensis under tropical conditions of India.

Keywords

AUDPC Cucumber Detached leaf assay Downy mildew Haemocytometer Sporulation 

Notes

Acknowledgements

The first author is grateful to the Director, Indian Institute of Horticultural Research, Bangalore for their support and facilities provided during this Ph.D. study. The first author thank Dr. Venugopalan R, Indian Institute of Horticultural Research, Bangalore for their helpful suggestions during statistical analysis.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflicts of interest.

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Copyright information

© Indian Phytopathological Society 2018

Authors and Affiliations

  1. 1.Division of Vegetable CropsICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  2. 2.Division of Plant PathologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  3. 3.Division of BiotechnologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia

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