Indian Phytopathology

, Volume 71, Issue 3, pp 337–348 | Cite as

Identification and confirmation of downy mildew (Pseudoperonospora cubensis Berk. & Curt.) resistance sources in cucumber (Cucumis sativus L.)

  • J. C. Bommesh
  • M. PitchaimuthuEmail author
  • A. T. Sadashiva
  • S. Sriram
  • B. Varalakshmi
  • K. V. Ravishankar
Research Article


An experiment was designed to identify the resistance source for downy mildew disease in different genotypes of cucumber. Forty-one cucumber genotypes were screened under natural condition and also under artificial epiphytic condition using detached leaf assay method. Genotype IIHR-438 and Cucumis metuliferus L. showed field resistance with an average PDI of 17.66 and 17.46; AUDPC of 772.24 and 764.48, respectively compared to 73.12 PDI and AUDPC of 3096.64 in highly susceptible genotype of IIHR-389. The disease reaction in selected genotypes of cucumber confirmed by artificial screening was in accordance with disease reaction under natural conditions. Resistant genotype IIHR-438 (14.3 PDI) and C. metuliferus L. (12.8 PDI) had least average PDI as compared to susceptible check Swarna Agethi (58.00 PDI) under artificial condition. Statistical analysis of disease severity data over a period for all the forty-one genotypes using non-linear growth model revealed that 99% variability in disease progression. Screening of genotypes under field conditions, sporulation of pathogen, progress of disease, detached leaf assay and non-linear statistical analysis implied that none of genotypes were found to be immune to downy mildew. Wherein the genotype, IIHR-438 and wild cucumber (C. metuliferus L.) were found resistant to downy mildew disease. Hence, it can be utilized in breeding program to develop resistant cultivar in cucumber against Pseudoperonospora cubensis under tropical conditions of India.


AUDPC Cucumber Detached leaf assay Downy mildew Haemocytometer Sporulation 



The first author is grateful to the Director, Indian Institute of Horticultural Research, Bangalore for their support and facilities provided during this Ph.D. study. The first author thank Dr. Venugopalan R, Indian Institute of Horticultural Research, Bangalore for their helpful suggestions during statistical analysis.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflicts of interest.


  1. Bhutia TL, Munshi AD, Behera TK, Sureja AK, Lal SK, Sinha P (2005) Genetics of yield traits and downy mildew resistance in cucumber (Cucumis sativus L.) Ph.D. thesis, Indian Agricultural Research Institute, New Delhi, p 37Google Scholar
  2. Bjoern GK, Kampmann HH (2000) Screening field Riesenschael cucumbers for resistance to downy mildew: management of inter plot interference problems. Acta Hortic 510:77–80. CrossRefGoogle Scholar
  3. Call AD (2012) Inheritance of resistance to downy mildew in cucumber (Cucumis sativus L.) PI 197088 and effect of interaction of host plant resistance, fungicides and environment on severity of downy mildew on cucumber. Ph.D. thesis, North Carolina State University, Raleigh, pp 86–88Google Scholar
  4. Cohen Y (1977) The combined effects of temperature, leaf wetness, and inoculum concentration on infection of cucumbers with Pseudoperonospora cubensis. Can J Bot 55:1478–1487. CrossRefGoogle Scholar
  5. Cohen Y, Eyal H (1977) Growth and differentiation of sporangia and sporangiophores of Pseudoperonospora cubensis on cucumber cotyledons under various combinations of light and temperature. Physiol Mol Plant Pathol 10:93–96. CrossRefGoogle Scholar
  6. Cohen Y, Petrov L, Baider A (2000) A leaf-disc bioassay for screening cucumbers for resistance to downy mildew. Acta Hortic 510:277–282. CrossRefGoogle Scholar
  7. Cohen Y, Meron I, Mor N, Zuriel S (2003) A new pathotype of Pseudoperonospora cubensis causing downy mildew in cucurbits in Israel. Phytoparasitica 31:458–466. CrossRefGoogle Scholar
  8. Criswell AD, Wehner TC, Klosinska U, Kozik E (2008) Use of sporulation and other leaf and vine traits for evaluation of resistance to downy mildew in cucumber. In: Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae. pp 23–27Google Scholar
  9. Dhillon N, Pushpinder PS, Ishiki K (1999) Evaluation of landraces of cucumber (Cucumis sativus L.) for resistance to downy mildew (Pseudoperonospora cubensis). Plant Genet Resour Newsl 119:59–61Google Scholar
  10. FAO (2016) FAO statistical yearbook. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  11. Jeger MJ, Rollinson SLH (2001) The use of the area under disease progress curve (AUDPC) to assess quantitative disease resistance in crop cultivar. Theor Appl Genet 102:32–40. CrossRefGoogle Scholar
  12. Jenkins SF, Wehner TC (1983) A system for the measurement of foliar diseases in cucumbers. Rep Cucurbit Genet Coop 6:10–12Google Scholar
  13. Kvalzeth TO (1985) Cautionary note about R 2. Am Stat 39:279–285Google Scholar
  14. Lebeda A (1992) Screening of wild Cucumis species against downy mildew (Pseudoperonospora cubensis L.) isolates from cucumbers. Phytoparasitica 20(3):203–210. CrossRefGoogle Scholar
  15. Lebeda A (1999) Pseudoperonospora cubensis L. on Cucumis spp. and Cucurbita spp.—resistance breeding aspects. Acta Hortic 492:363–370. CrossRefGoogle Scholar
  16. Lebeda A, Cohen Y (2011) Cucurbit downy mildew (Pseudoperonospora cubensis L.) biology, ecology, epidemiology, host-pathogen interaction and control. Eur J Plant Pathol 129:157–192. CrossRefGoogle Scholar
  17. Lebeda A, Prasil J (1994) Susceptibility of Cucumis sativus L. cultivars to Pseudoperonospora cubensis L. Acta Phytopathol 29:89–94Google Scholar
  18. Lebeda A, Urban J (2007) Temporal changes in pathogenicity and fungicide resistance in Pseudoperonospora cubensis L. populations. Acta Hortic 731:327–336. CrossRefGoogle Scholar
  19. Mhada M, Ezzahiri B, Benlhabib O (2015) Assessment of downy mildew resistance (Peronospora farinosa) in a Quinoa (Chenopodium quinoa Wild.) Germplasm. Int J Biol Med Res 6(1):4748–4752Google Scholar
  20. Nagarajan S, Muralidharan K (1995) Dynamics of plant diseases. Allied publisher, New DelhiGoogle Scholar
  21. Nayak P, Mukherjee AK, Pandit E, Pradhan SA (2018) Application of statistical tools for data analysis and interpretation in rice plant pathology. Rice Sci 25(1):1–18. CrossRefGoogle Scholar
  22. Neufeld KN, Ojiambo PS (2012) Interactive effects of temperature and leaf wetness duration on sporangia germination and infection of cucurbit hosts by Pseudoperonospora cubensis. Plant Dis 96(3):345–353. CrossRefGoogle Scholar
  23. Neykov S, Dobrev D (1987) Introduced cucumber cultivars relatively resistant to Pseudoperonospora cubensis in Bulgaria. Acta Hortic 220:115–119. CrossRefGoogle Scholar
  24. Patil PV (1997) Studies on sunflower rust caused by Puccinia helianthi schw. Ph.D. thesis, University of Agricultural Sciences, Dharwad, p 238Google Scholar
  25. Petrov L, Boodert K, Sheck L, Baider A, Rubin E, Cohen Y, Datzir N, Paris HS (2000) Resistance to downy mildew, Pseudoperonospora cubensis in cucumbers. Acta Hortic 510:203–209CrossRefGoogle Scholar
  26. Ratkowsky DA (1990) Handbook of nonlinear regression models. Marcel Dekker, New YorkGoogle Scholar
  27. Reddy NS (2002) Biochemical mechanism of downy mildew resistance in musk melon (Cucumis melo L.) caused by Pseudoperonospora cubensis (Berk and Curt) Rostow. M.Sc. thesis, University of Agricultural Sciences, Bangalore, p 21Google Scholar
  28. Reshmi (2006) Morphological, molecular diversity and evaluation of developed F1·hybrids for resistance to powdery and downy mildew diseases in Cucumber (Cucumis sativus L.) Ph.D. thesis, University of Agricultural Sciences, Bangalore, p 180Google Scholar
  29. Seshadri VS (1986) Cucurbits. In: Bose TK, Som MG (eds) Vegetable crops in India. Studium Press, New Delhi, p 91Google Scholar
  30. Siegel S, Castellan ND (1988) Nonparametric statistics for behavioral sciences. McGraw-Hill, New YorkGoogle Scholar
  31. Sinha P, Prajneshu Varma A (2002) Growth models for powdery mildew development of mango. Ann Plant Prot Sci 10:84–87Google Scholar
  32. Thomas CE (1977) Influence of dew on downy mildew of cantaloupe in South Texas. Phytopathology 67:1368–1369CrossRefGoogle Scholar
  33. Thomas CE (1996) Downy mildew. In: Zitter TA (ed) Compendium of cucurbit diseases. Cornell University Press, New York, pp 25–27Google Scholar
  34. Thomas CE, Inaba T, Cohen Y (1987) Physiological specialization in Pseudoperonospora cubensis. Phytopathology 77:1621–1624CrossRefGoogle Scholar
  35. Urban J, Lebeda A (2006) Fungicide resistance in cucurbit downy mildew—methodological, biological and population aspects. Ann Appl Biol 149:63–75CrossRefGoogle Scholar
  36. Van der Plank JE (1968) Plant diseases, epidemics and control. Academic Press, New YorkGoogle Scholar
  37. Venugopalan R, Vijay N (2015) Nonlinear logistic model for describing downy mildew incidence in grape. J Indian Soc Agric Stat 69(1):19–25Google Scholar
  38. Wan H, Zhao Z, Malik AA, Qian C, Chen J (2010) Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in Cucumis. BMC Plant Biol 10:186. CrossRefPubMedPubMedCentralGoogle Scholar
  39. Wehner TC, Shetty NV (1997) Downy mildew resistance of the cucumber germplasm collection in North Carolina field tests. Crop Sci 37:1331–1340CrossRefGoogle Scholar
  40. Wilcoxson RD, Skovmand B, Atif AH (1975) Evaluation of wheat cultivars for ability to retard development of stem rust. Ann Appl Biol 80:275–281. CrossRefGoogle Scholar

Copyright information

© Indian Phytopathological Society 2018

Authors and Affiliations

  1. 1.Division of Vegetable CropsICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  2. 2.Division of Plant PathologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  3. 3.Division of BiotechnologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia

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