Efficacy of URP and ISSR markers to determine diversity of indigenous and exotic isolates of Curvularia lunata
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The efficacy of universal rice primer (URP) and inter simple sequence repeat (ISSR) markers was assessed in genetic diversity analysis among ten diverse isolates of Curvularia lunata. The parameters like percentage polymorphism (PP), polymorphism information content (PIC), expected heterozygosity (He), resolution power (RP), Shannon’s information index (I), effective multiplex ratio (EMR), marker index (MI) and cophenetic matrix were used in the study. The PIC, PP, He, RP and I values were nearly identical for both marker systems while, EMR, MI and cophenetic correlation coefficient were found to be higher in ISSR than URP marker system. Based on these parameters, ISSR markers were proved to be more efficient than URP markers in diversity analysis of C. lunata. Combined markers based dendrogram, grouped all the isolates of C. lunata into six clusters. Indigenous isolates except one form one cluster while most of the exotic isolates form separate clusters based on their geographical regions. The combine analysis of URP and SRAP markers showed high cophenetic correlation coefficient (r = 0.942), which indicated that combined markers analysis is giving better results for genetic diversity study.
KeywordsCurvularia lunata Dendrogram Genetic diversity ISSR URP
Authors gratefully acknowledge the Director, ICAR-NBPGR for support and encouragement and Indian Council of Agricultural Research (ICAR), New Delhi for financial support.
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