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Phytochemical Exploration and Investigation of In Vitro Antioxidant and Antibacterial Activities of Different Solvent Extracts of Clematis Hirsuta (Nech Yeazohareg) Leaves

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Clematis hirsuta is one of the commonly used traditional medicinal plants by different communities in Ethiopia for treating wounds, headaches, and coughs. The current study intended to explore qualitative phytochemical contents, estimation of total phenolic and total flavonoid contents, purification, antioxidant and antibacterial efficiency of the petroleum ether, chloroform, ethanol crude extract, and semi-solid fractions(from chloroform extract) of the leaves of C. hirsuta. The probe into antioxidant activities using DPPH and FRAP assays showed that the ethanol extract of the leaves showed the highest antioxidant activity of 82.9% with an IC 50% value of 43.2 and 36.9% with an IC 50% value of 118.3 respectively. These results are in apparent agreement with the higher phenolic and flavonoid contents (163.6 mg GA/g, and 161.0 mgCE/g of sample), respectively, of the ethanol extract. The chloroform extract exhibited higher antibacterial efficiency against all tested bacterial strains. Solid fractions were also active against the given bacterial strains with MIC ranging from 15.64 to 62.50 ppm. This study provides the fact that the leaves extract of C. hirsuta could be a better source of natural antioxidants and antibacterial agents.

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Authors and Affiliations



AzMA SLB: Conception and design of study; AzMA, AnMA, MTA: acquisition of data; AzMA, MTA, AnMA, BBB and SLB: analysis and/or interpretation of data; MTA, BBB, MAK, AnMA, AzMA: drafting the manuscript; MTA, SLB, BBB, MAK: Revising the manuscript critically for important intellectual content. All authors revised the manuscript.

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Correspondence to Misganaw T. Ayana.

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Ethical Approval

Using plants without scientific knowledge is sometimes hazardous because of unbalanced doze. The process that used in this study doesn’t affect any leaving matter especially Humans, Animals and Plants. And no human identity revealing data is used in the study.

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Adane, A.M., Ayana, M.T., Bikilla, S.L. et al. Phytochemical Exploration and Investigation of In Vitro Antioxidant and Antibacterial Activities of Different Solvent Extracts of Clematis Hirsuta (Nech Yeazohareg) Leaves. Chemistry Africa 6, 855–866 (2023).

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