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Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction

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A Correction to this article was published on 22 April 2022

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Inflammation continues to become a growing health concern linked to different disease conditions worldwide. This study aimed to validate the ethnomedicinal topical anti-inflammatory activity of Petiveria alliacea and elucidate the possible inflammatory inhibitory mechanism of the chemical constituents identified from the most active fraction. The egg-albumin induced rat paw oedema model was used to evaluate the anti-inflammatory activity of P. alliacea leaf extract and partitioned fractions. The GC–MS chemical profiling of the most active fraction identified the chemical constituents responsible for the anti-inflammatory activity. Furthermore, a molecular docking study of TNF-α and COX-2 enzymes were carried out followed by the DFT studies of the hit molecules. The findings showed that P. alliacea extract at 2.5% and 5% elicited anti-inflammatory effect greater than that of the standard drug. The n-hexane fraction showed greater oedema inhibition after 4 h as compared to the aqueous fraction and diclofenac gel. The GC–MS analysis of the n-hexane fraction revealed the presence of 19 chemical compounds. Of the identified chemical compounds, Stigmasterol (− 6.5 kcal/mol) elicited the best binding energy against TNF- α, while Stigmasterol (− 9.7 kcal/mol) and vitamin E (− 8.7 kcal/mol) were identified as hit molecules against COX-2 enzyme. Also, the electronic parameters calculated revealed the compounds as promising anti-inflammatory molecules. The study suggests extensive phytochemical and pharmacological investigation of the most active fraction to identify new anti-inflammatory agents.

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Authors and Affiliations



Conceptualization, KOF and OGO; methodology, KOF, BIO and OGO; data analysis, OGO and OIA; GC–MS data acquisition, AHA; molecular docking and DFT studies, EGF and KOF; investigation, OGO, BIO and KOF; writing of original draft; OGO, BIO, AHA, OIA, GA and KOF writing, reviewing and editing, KOF, AHA and GA; supervision, OGO and KOF. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Kolade O. Faloye.

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The original online version of this article was revised to correct author name to Kolade O. Faloye and correct values in abstract.

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Olajubutu, O.G., Ogunremi, B.I., Adewole, A.H. et al. Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction. Chemistry Africa 5, 557–565 (2022).

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