Abstract
The essential oils of plants have been widely recognized in the pharmaceutical industry due to their various benefits as well as their biological activities. In Algeria, the seeds of Foeniculum vulgare Mill (Family Apiaceae) are widely utilized in herbal medicine. We aimed to examine the chemical composition of seed essential oil of this species, and to reveal its in vitro antifungal activity against Candida albicans, Aspergillus flavus, Aspergillus niger, Rhizopus sp., Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton violaceum, Microsporumgypseum, Microsporumcanis and Rhodotorula sp. Chemical analysis of essential oil isolated by hydro-distillation was carried out by GC–FID and GC–MS. The basic components of the essential oil were estragole (84.8%), limonene (7.8%), fenchone (3.1%) and α-pinene (1.3%). The essential oil of F. vulgare revealed higher antifungal activities particularly against C. albicans and Aspergillus species with MIC values of 0.16–0.2 mg/ml.
Similar content being viewed by others
References
Muckensturm B, Foechterlen D, Reduron JP, Danton P, Hildenbrand M (1997) Phytochemical and chemotaxonomic studies of vulgare. Biochem Syst Ecol 25(4):353–358
Ozcan MM, Chalchat JC (2006) Effect of collection time on Chemical composition of the essential oil of Foeniculum vulgare subsp. piperitum growing wild in Turkey. Eur Food Res Technol 224:279–281
Rather MA, Dar BA, Sofi SN, Bhat BA, Qurishi MA (2016) Foeniculum vulgare: a comprehensive review of its traditional use, phytochemistry, pharmacology, and safety. Arab J Chem 9(2):1574–1583
Al-Snafi AE (2018) The chemical constituents and pharmacological effects of Foeniculum vulgare—a review. J Pharm 8(5):81–96
He W, Huang B (2011) A review of chemistry and bioactivities of medicinal spice Foeniculum vulgare. J Med Plants Res 5(16):3595–3600
Kaur GJ, Arora DS (2010) Bioactive potential of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi belonging to the family Umbelliferae—current status. J Plants Res 4(2):087–094
Celik I, Isik I (2008) Determination of Chemopreventive role of Foeniculum vulgare and Salvia officinalis infusion on trichloroacetic acid-induced increased serum marker enzymes lipid peroxidation and antioxidative defense systems in rats. Nat Prod Res 22:66–75
Birdane FM, Cemek M, Birdane YO, Gulcin I, Buyukokuroglu ME (2007) Beneficial effects of Foeniculum vulgare on ethanol/induced acute gastric mucosal injury in rats. World J Gastroenterol 13:607–611
Albert-puleo M (1980) J Ethnopharmacol 2:337
Liu J, Luo JG, Ye H, Sun Y, Lu ZX, Zeng XX (2010) Medium optimization and structural characterization of exopolysaccharides from endophytic bacterium Paenibacilluspolymysa EJS-3. Carbohydr Polym 79:206–213
Ruberto G, Tiziana Baratta M, Stanley Deans G, Damien Dorman HJ (2000) Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmumm aritimum essential oils. Planta Med 66:687–693
Adel FA, Mengjin S, Cunyu L, Wenyi K (2019) Comparative analysis of antioxidant activities of essential oils and extracts of fennel (Foeniculum vulgare Mill.) seed from Egypt and China. Food Sci Hum Wellness 8:67–72
Abdurahim SA, Elamin BEK, Ali B, Almagboul AZ (2017) In vitro test of antimicrobial activity of Foeniculum vulgare Mill. (Fennel) essential oil. JMESS 3(4):1609–1614
Aamir F, Bashir H, Mahmood MV (2018) Antifungal activity of freshly growing seeds of fennel (Foeniculum vulgare). PJMHS 12(4):1487–1488
Bassyouni RH, Wali IE, Kamel Z, Kassim MF (2019) Fennel oil: a promising antifungal agent against biofilm forming fluconazole resistant Candida albicans causing vulvovaginal coulddidiasis. J Herb Med 15:100–227
Foroughi A, Pournaghi P, Zhaleh M, Zangeneh A, Zangeneh MM, Moradi R (2016) Antibacterial activity and phytochemical screening of essential oil of Foeniculum vulgare. Int J Pharm Clin Res 8(11):1505–1509
Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2017) Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rev Res 8(1):13–17
Choi EM, Hwang JK (2004) Anti-inflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia 75:557–565
Lee HS, Kang P, Kim KY, Seol GH (2015) Foeniculum vulgare Mill. protects against lipopolysaccharide-induced acute lung injury in mice through ERK-dependent NF-KB activation. Korean J Physiol Pharmacol 19:183–189
Ozbek H, Ugras S, Dulger H, Bayram I, Tuncer I, Ozturk G, Ozturk A (2003) Hepatoprotective effect of Foeniculum vulgare essential oil. Fitoterapia 74:317–319
Sheharbano B, Syed AAS, Touqeer A, Saadia Z (2018) Neuroprotective effects of Foeniculum vulgare seeds extract on lead induced neurotoxity in mice brain. Drug Chem Toxicol 41(4):399–407
Council of Europe (1997) European Pharmacopoeia, 3rd edn. Council of Europe Press, Strasbourg, p 121
NIST/EPA/NIH (2005) Mass spectrallibrary. National Institute of Standard and Technology, Gaithersburg
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Carol Stream
Van Den Dool H, Kratz PD (1963) A generalization of the retention index system including linear temperature programmed gas–liquid partition chromatography. J Chromatogr 11:463–471
National Committee for Clinical Laboratory Standards (2002) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Approved standard M38A. Wayne
Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Faiza M, Hammouda FM, Abdel-Rahman FH, Saleh MA (2011) Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules 16:1366–1377
Aćimović M, TesevićV Todosijević M, Djisalov J, Oljača S (2015) Compositional of the essential oil of Pimpinella anisum and Foeniculum vulgare grown in Serbia. Bot Serb 39(1):9–14
Garzoli S, Bozovic M, Baldisserotto A, Sabatino M, Cesa S, Pepi F, Vicentini CB, Manfredini S, Ragno R (2018) Essential oil extraction, chemical analysis and anti-candida activity of Foeniculum vulgare Miller-new approaches. Nat Prod Res 32(11):1254–1259
Mimica-Dukié N, Kujundzie S, Sokovie M, Couladis M (2003) Essential oil composition and antifungal activity of Foeniculum vulgare Mill. obtained by different distillation conditions. Phytother Res 17:368–371
Ilić DP, Stanojević LP, Troter DZ, Stanojević JS, Danilović BR, Nikolić VD, Nikolić LB (2019) Improvement of the yield and antimicrobial activity of fennel (Foeniculum vulgare Mill.) essential oil by fruit milling. Ind Crops Prod. https://doi.org/10.1016/J.indccrop111845
Telci I, Demirtas I, Sahin A (2009) Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Ind Crops Prod 30(1):126–130
Saxena SN, Agarwal D, John S, Dubey PN, Lal G (2018) Analysis of fennel (Foeniculum vulgare) essential oil extracted from green leaves, seeds and dry straw. Int J Seed Spices 8(1):60–64
Diaaz-Maroto MC, Pea rez-Coello MS, Esteban J, Sanz J (2006) Comparison of the volatile composition of wild fennel samples (Foeniculum vulgare Mill.) from Central Spain. J Agric Food Chem 54:6814–6818
Gross M, Lewinsohn E, Tadmor Y, Bar E, Dudai N, Cohen Y, Friedma J (2009) The inheritance of volatile phenylpropenes in bitter fennel (Foeniculum vulgare Mill. var. vulgare Apiaceae) chemotypes and their distribution within the plant. Biochem Syst Ecol 37:308–316
Piccaglia R, Marotti M (2001) Characterization of some Italian types of wild Fennel (Foenilum vulgare Mill). J Agric Food Chem 49:239–244
Zeng H, Chen X, Liang J (2015) In vitro antifungal activity and mechanism of essential oil from fennel (Foeniculum vulgare L.) on dermatophyte species. J Med Microbiol 64:93–103
Ozgan MM, Chalchat JC, Arslan D, Ates A, Unver A (2006) Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation. J Med Food 9(4):552–561
Keskin I, Gunal Y, Ayla S, Kolbasi B, Sakul A, Kilic U, Gok O, Koroglu K, Ozbek H (2017) Effects of Foeniculum vulgare essential oil compounds, fenchone and limonene, on experimental wound healing. Biotech Histochem 92(4):274–282
Soylu S, Yigitbas H, Soylu EM (2007) Antifungal effects of essential oils from oregano and fennel on Sclerotinia sclerotiorum. J Appl Microbiol 103:1021–1030
Shaker GA, Alhamadany HS (2015) Isolation and identification of fungi which infect fennel Foeniculum vulgare mill. and its impact as antifungal agent. Bull Iraq Nat Hist Mus 13(2):31–38
Kazemi M, Mousavi E, Kharestani H (2012) Chemical compositions and antimicrobial activities of essential oils of Varthemiapersica, Foeniculum vulgare and Ferulalycia. Curr Res Bacteriol 5:42–52
Acknowledgements
We would like to express our gratitude to Professor Silvia Porcedda for welcoming us to the Department of Chemical and Geological Sciences, University of Cagliari, as well as for her help and guidance. The authors would like to acknowledge Professor Meghit Boumediene Khaled for providing necessary corrections to the last version of the article.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Belabdelli, F., Piras, A., Bekhti, N. et al. Chemical Composition and Antifungal Activity of Foeniculum vulgare Mill. Chemistry Africa 3, 323–328 (2020). https://doi.org/10.1007/s42250-020-00130-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42250-020-00130-x