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5-Azacytidine incorporated chitosan/collagen/gold nanoparticle matrix preparation and characterization with potential to repair myocardial infarction

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Abstract

With cardiovascular disease has remained a leading cause of death and treatment modalities for myocardial tissue repair have provided promise and life to damaged heart tissue patients. Biomaterials, including natural and synthetic polymers, have shown effectiveness towards cell differentiation and proliferation. Small amounts of collagen in the matrix increase the biocompatibility and biodegradability, and thus the matrix employed in the study utilized a composition consisting of chitosan (CNPs) and collagen (Coll). In this study, gold nanoparticles (GNP) were added to the matrix to enhance the electrical conductivity to improve the heart function. 5-Azacitidine (5-Aza) was incorporated into the matrix to induce myogenic differentiation. The prepared matrix of GNP-5-Aza@CNPs@Coll thus had a hydrodynamic diameter of 382 nm and an electrical conductivity of 120.2 μs/cm. Loading of 5-Aza and gold nanoparticles in the matrix has been confirmed through HPLC and ICP-OES analyses, while FTIR bands confirmed the fingerprint of each component of composite. Biocompatibility of the matrix has been established through hemolysis assays. Animal studies revealed promising application of the matrix as injectable formulations for myocardial tissue engineering. In essence, the study opens a promise for otherwise challenged myocardial tissue engineering, through appropriate combination of chitosan, collagen, 5-Aza, and gold nanoparticles, leading to a biocompatible and potentially biodegradable matrix.

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The datasets generated during and/or analyzed during the study are available on reasonable request.

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Acknowledgements

The authors thank Chettinad Academy of Research and Education for funding student project. We thank CSIR-CLRI and IIT Madras for instrumentation facilities.

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Authors and Affiliations

  1. Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai, Tamil Nadu, 603103, India

    R Nikitha Shalom, Shoba Narayan, N Sangamithra, P Soniya, P Subitha,  Geeva & Ramachandran Murugesan

  2. Department of Cardiology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai, Tamil Nadu, 603103, India

    M. Chokkalingam

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  1. R Nikitha Shalom
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  2. Shoba Narayan
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  3. N Sangamithra
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  4. P Soniya
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  5. P Subitha
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  6. Geeva
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  8. Ramachandran Murugesan
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Correspondence to Shoba Narayan.

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All experiments executed according to the guidelines and approved by the institutional animal ethics committee and institutional human ethical committee.

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Nikitha Shalom, R., Narayan, S., Sangamithra, N. et al. 5-Azacytidine incorporated chitosan/collagen/gold nanoparticle matrix preparation and characterization with potential to repair myocardial infarction. emergent mater. 6, 1563–1576 (2023). https://doi.org/10.1007/s42247-023-00534-8

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  • DOI: https://doi.org/10.1007/s42247-023-00534-8

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