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Rapid synthesis of novel cerium oxide microtubes and its cytocompatibility study

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Abstract

Microtubes (MT) are one of the emerging biomaterials as they can play an important role as porous conduits, guidance cues, microvascular joining, and places where tissue composition matches microtubular geometry. In this regard, protein- and polymeric-based MT have reported biodegradable protein conduit for nerve repair and silk micro-vasculature-based repair of the blood vessel. Here in this article, we report for the first time the rapid production method of cerium oxide microtubes (CeO2-MT) and their cytocompatibility with C2C12 cells. The microtubes were prepared by precipitating CeO2 in an aqueous solution on the electrospun polycaprolactone (PCL) nanofibers, followed by thermal degradation at 440 °C for 5 h to remove the PCL, thereby obtaining the CeO2-MT. X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, Raman spectroscopy, and Brunauer–Emmett–Teller (BET) surface area measurements confirmed the presence of MT with the phase of CeO2, with fiber diameter ≈280 nm and high surface area due to tubular space. MT was cytocompatible with mouse myoblast cell line C2C12 cells and protected the cells from reactive oxygen species (ROS) insult at 100 µM H2O2 concentration. Therefore, the findings demonstrate the potentiality of MT tubular structure as novel biomaterials for scavenging high ROS and as a guided cell cue for potential muscle tissue regeneration.

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Data Availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are thankful to the National Institute of Technology, Raipur (Chhattisgarh), India, and (CeNSE- MNCF) IISc Bangalore for providing support for this work. CM acknowledges the National Institute of Technology Raipur for the Seed Grant, Project No: NITRR/Seed Grant/2021-22/30. CM also gratefully acknowledged research support from the Department of Science and Technology, India, Science and Engineering Research Board (SERB), Sanction Order No SRG/2022/000348.

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  1. Department of Biotechnology, National Institute of Technology Raipur, Raipur, Chhattisgarh, 492010, India

    Dilip Kumar Chandra, Awanish Kumar & Chinmaya Mahapatra

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Contributions

C.M. conceived and designed the study. D.C. performed the experiments. A.K. and C.M analyzed the data and wrote first draft of the manuscript. All the authors reviewed the manuscript. C.M. prepared the final draft of the manuscript.

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Correspondence to Chinmaya Mahapatra.

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Highlights

1. Unique method for rapid production of cerium microtubes is reported.

2. Cerium oxide microtubes are cytocompatible with C2C12 cells.

3. Cerium oxide microtubes could provide directional cue to the C2C12 cells.

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Chandra, D.K., Kumar, A. & Mahapatra, C. Rapid synthesis of novel cerium oxide microtubes and its cytocompatibility study. emergent mater. 6, 595–603 (2023). https://doi.org/10.1007/s42247-023-00498-9

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