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Adhesion-enhancing coating embedded with osteogenesis-promoting PDA/HA nanoparticles for peri-implant soft tissue sealing and osseointegration

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Abstract

Following dental implantation, the characteristic bacterial milieu of the oral cavity may lead to peri-implant inflammation, which can negatively impact osseointegration and cause implant failure. To improve soft tissue sealing around the implant, enhance osseointegration, and improve implant success rates, this paper proposes a composite multifunctional coating (PHG) prepared using gelatin and polydopamine/hydroxyapatite nanoparticles, investigates the effects of this novel coating on cell adhesion, proliferation, antibacterial activity, osteogenic differentiation, and evaluates its immune-related properties. The PHG coating was proved to have satisfactory hydrophilicity and wettability for cell attachment. Furthermore, it improved the expression of adhesion-related genes and proteins in human gingival fibroblasts, indicating its adhesion-promoting effect. Additionally, bone marrow mesenchymal stem cells exhibited strong osteogenic differentiation potential and mineralization on PHG-coated surfaces. Notably, the PHG coating exhibited antibacterial activity against Streptococcus mutans, as well as anti-inflammatory effects, potentially via the regulation of macrophages. Therefore, the proposed PHG coating may promote soft tissue sealing and bone bonding, providing a potential strategy for the surface modification of dental implants.

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Acknowledgements

This research was funded by the National Natural Science Foundation of China (Nos. 81801006, 31870953, 81901048, 81620108006, 81991505, 81921002, 81801023, and 82100963), Shanghai Rising-Star Program (21QA1405400), the National Key Research and Development Program of China (No. 2016YFC1102900), and Innovative Research Team of High-Level Local Universities in Shanghai (No. SSMU-ZDCX20180900).

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  1. Tingshu Su and Ao Zheng have contributed equally to this work.

Authors and Affiliations

  1. Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Tingshu Su, Ao Zheng, Lingyan Cao, Lingjie Peng, Xiao Wang, Jie Wang, Xianzhen Xin & Xinquan Jiang

  2. College of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Jiao Tong University, Shanghai, 200011, China

    Tingshu Su, Ao Zheng, Lingyan Cao, Lingjie Peng, Xiao Wang, Jie Wang, Xianzhen Xin & Xinquan Jiang

  3. Shanghai Key Laboratory of Stomatology, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai, 200011, China

    Tingshu Su, Ao Zheng, Lingyan Cao, Lingjie Peng, Xiao Wang, Jie Wang, Xianzhen Xin & Xinquan Jiang

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  1. Tingshu Su
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Contributions

Conceptualization, XZX and AZ; methodology, TSS; software, XW; data curation, LJP; writing—original draft preparation, AZ; writing—review and editing, LYC; visualization, AZ and JW; funding acquisition, XZX and XQJ. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Xianzhen Xin or Xinquan Jiang.

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The authors declare that there were no financial or non-financial interests that are directly or indirectly related to the work submitted for publication.

Ethical approval

All institutional and national guidelines for the care and use of laboratory animals were followed. All experimental protocols involving animals were approved by the Institutional Animal Care and Use Committee and followed the procedure for Animal Experimental Ethical Inspection of the Ninth People’s Hospital, which is affiliated with Shanghai Jiao Tong University School of Medicine (SCXK (Shanghai) [201866]).

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Su, T., Zheng, A., Cao, L. et al. Adhesion-enhancing coating embedded with osteogenesis-promoting PDA/HA nanoparticles for peri-implant soft tissue sealing and osseointegration. Bio-des. Manuf. 5, 233–248 (2022). https://doi.org/10.1007/s42242-022-00184-5

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