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Additive nanomanufacturing of lab-on-a-chip fluorescent peptide nanoparticle arrays for Alzheimer’s disease diagnosis

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Abstract

This paper proposes an additive nanomanufacturing approach to fabricate a personalized lab-on-a-chip fluorescent peptide nanoparticles (f-PNPs) array for simultaneous multi-biomarker detection that can be used in Alzheimer’s disease (AD) diagnosis. We will discuss optimization techniques for the additive nanomanufacturing process in terms of reliability, yield and manufacturing efficiency. One contribution of this paper lies in utilization of additive nanomanufacturing techniques to fabricate a patient-specific customize-designed lab-on-a-chip device for personalized AD diagnosis, which remains a major challenge for biomedical engineering. Through the integrated bio-design and bio-manufacturing process, doctor’s check-up and computer-aided customized design are integrated into the lab-on-a-chip array for patient-specific AD diagnosis. In addition, f-PNPs with targeting moieties for personalized AD biomarkers will be self-assembled onto the customized lab-on-a-chip through the additive nanomanufacturing process, which has not been done before. Another contribution of this research is the personalized lab-on-a-chip f-PNPs array for AD diagnosis utilizing limited human blood. Blood-based AD assessment has been described as “the holy grail” of early AD detection. This research created the computer-aided design, fabrication through additive nanomanufacturing, and validation of the f-PNPs array for AD diagnosis. This is a highly interdisciplinary research contributing to nanotechnology, biomaterials, and biomedical engineering for neurodegenerative disease. The conceptual work is preliminary with intent to introduce novel techniques to the application. Large-scale manufacturing based on the proposed framework requires extensive validation and optimization.

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Authors and Affiliations

  1. Department of Biomedical Engineering, College of Engineering, Ohio State University, Columbus, OH, 43210, USA

    Leming Sun & Mingjun Zhang

  2. School of Life Sciences, Northwestern Polytechnical University, Xi’an, 710072, Shaanxi, China

    Leming Sun

  3. Department of Polymeric Materials, School of Material Science and Engineering, Tongji University, Shanghai, 201804, China

    Zhen Fan

  4. Institute for Advanced Study, Tongji University, Shanghai, 200092, China

    Zhen Fan

  5. School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, 200444, China

    Tao Yue

  6. The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310028, China

    Jun Yin & Jianzhong Fu

  7. Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310028, China

    Jun Yin & Jianzhong Fu

  8. Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, 43210, USA

    Mingjun Zhang

  9. Interdisciplinary Biophysics Graduate Program, Ohio State University, Columbus, OH, 43210, USA

    Mingjun Zhang

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  1. Leming Sun
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  2. Zhen Fan
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  3. Tao Yue
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Correspondence to Leming Sun.

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Sun, L., Fan, Z., Yue, T. et al. Additive nanomanufacturing of lab-on-a-chip fluorescent peptide nanoparticle arrays for Alzheimer’s disease diagnosis. Bio-des. Manuf. 1, 182–194 (2018). https://doi.org/10.1007/s42242-018-0019-9

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  • DOI: https://doi.org/10.1007/s42242-018-0019-9

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