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Comparative Study between Bone Tissue Engineering Scaffolds with Bull and Rat Cancellous Microarchitectures on Tissue Differentiations of Bone Marrow Stromal Cells: A Numerical Investigation

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Abstract

Tissue-engineered bone scaffolds provide temporary mechanical support for bone tissue growth. Mechanical stimuli are transferred to seeded cells through the scaffold structure to promote cell proliferation and differentiation. This paper presents a numerical investigation specifically on bone and cartilage tissue differentiation with the aim to provide a theoretical basis for scaffold design and bone defect repair in clinics. In this study, the scaffold structures were established on the basis of cancellous bone microarchitectures. For finite element simulations, inlet velocity and compressive strain were applied under in vitro culture conditions. The influences of this scaffold morphology and macro-level culture conditions on micro-mechanical stimuli at scaffold surfaces were investigated. Correlations between the microarchitectural parameters and the mechanical parameters, as well as the cell differentiation parameters were analyzed. Highly heterogeneous stress distributions were observed on the scaffolds with irregular morphology. Cell differentiation on the scaffold was more sensitive to the inlet velocity than the axial strain. In addition, cartilage differentiation on the scaffolds with structures comprising more plate-like trabeculae was more pronounced than on those with more rod-like trabeculae. This paper is helpful to gain more insight into the mechanical environments under in vitro culture conditions that approximate the in vivo mechanical environments of Bone Marrow Stromal Cells (BMSCs).

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Acknowledgment

This work is supported by the National Natural Science Foundation of China (Nos. 81471753, 11432016 and 11322223), the project from Science and Technology Department of Jilin Province (20160101297JC), the project from Department of Education Science and Technology of Jilin Province (JJKH20180560KJ) and the grant from China Scholarship Council.

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Authors and Affiliations

  1. State Key Laboratory of Automotive Simulation and Control, Jilin University, Changchun, 130022, China

    Xianbin Zhang & He Gong

  2. Department of Engineering Mechanics, Jilin University, Changchun, 130022, China

    Xianbin Zhang & He Gong

  3. Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway

    Xianbin Zhang

  4. Department of Automotive Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China

    Ruoxun Fan

  5. School of Civil Engineering, Jilin Jianzhu University, Changchun, 130118, China

    Juan Fang

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  1. Xianbin Zhang
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Correspondence to He Gong.

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Zhang, X., Gong, H., Fan, R. et al. Comparative Study between Bone Tissue Engineering Scaffolds with Bull and Rat Cancellous Microarchitectures on Tissue Differentiations of Bone Marrow Stromal Cells: A Numerical Investigation. J Bionic Eng 15, 924–938 (2018). https://doi.org/10.1007/s42235-018-0079-8

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  • DOI: https://doi.org/10.1007/s42235-018-0079-8

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