Abstract
In the present study, we explored the disease-control efficacy of a powder preparation of the widely available evergreen medicinal shrub Dodonaea viscosa (L.) Jacquin against the bacterial wilt (BW) of tomato caused by R. solanacearum. Both in-vitro and in-planta experiments were conducted, evaluating different concentrations of D. viscosa dried powder against the BW pathogen at different time interval. Studies revealed that all tested concentrations of the product displayed significantly larger zones of the pathogen inhibition (ZIs) as compared to control treatments. D. viscosa methanol and water extracts at 15% produced 26.66 mm and 26.00 mm ZIs, respectively whereas the same products at 10% produced both 22 mm ZIs. Further, in-vitro inhibition tests were carried out using HPLC fractions. Results evidenced that fraction six produced 23 mm ZI followed by fraction five and eight that displayed 21.66 and 20.00 mm ZIs, respectively. Likewise, the longest degradation time (20 Days Before Transplanting, DBT) coupled with the highest tested dose (30 g powder preparation) produced higher activity respect to the control treatments, improving stem length, root length and fresh plant biomass by 102.3%, 74.3% and 105.8%, respectively. Likewise, the highest dose of D. viscosa powder thoroughly mixed with potted soil 10 days DBT significantly enhanced plant growth parameters such as stem length (+ 88.3%), root length (+ 87.1%) and fresh biomass (+ 96.8%) as compared to control. Our results suggest that 30 g dried powder of D. viscosa mixed well with soil artificially contaminated with the pathogen 20 DBT could be used as an effective tool in integrated disease management against BW of tomato.
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Data Availability
Data will be made available on request.
Abbreviations
- BW:
-
Bacterial wilt
- ZI:
-
Zone of inhibition
- DBT:
-
Days before transplanting
- IDM:
-
Integrated disease management
- OAs:
-
Organic amendments
- NA:
-
Nutrient agar
- DMSO:
-
Dimethyl sulfoxide
- SDW:
-
Sterilized distilled water
- CRD:
-
Completely randomised design
- AUDPC:
-
Area under disease progress curve
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Ahmad, K., Khan, R.A.A., Ahmad, M. et al. Evaluating powder preparation of Dodonaea viscosa as a new potential control measure against bacterial wilt of tomato. J Plant Pathol 105, 921–932 (2023). https://doi.org/10.1007/s42161-023-01403-3
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DOI: https://doi.org/10.1007/s42161-023-01403-3