Abstract
Seed-borne mycoflora can affect seed health and quality. The present study investigated the occurrence of seed-borne fungi in 600 seed samples of Avena sativa (B7) and A. nuda (B2) collected from Baicheng (BB), Dingxi (DB), and Haibei (HB) cities in China. Effects of seed-borne fungi on seed germination and seedling growth of oat were also studied. Among all isolated fungi, 10 fungal genera with high incidence were identified by colony color, spore morphology, and ITS sequence analysis, including Alternaria, Epicoccum, Fusarium, Bipolaris, Pyrenophora, Aspergillus, Cladosporium, Nigrospora, Pleospora, and Hypomyces. Alternaria spp. showed the highest incidence of 34.2%, while Nigrospora oryzae and Hypomyces sp. showed a lower incidence, 1.87% and 1.77%, respectively. The highest number of fungal species was isolated from HB-7 seeds compared to BB-2 which had the lowest species. Alternaria and Epicoccum were the dominant seed-borne species isolated from oat seeds in three regions, while B7 showed a higher infection incidence than B2. The pathogenicity tests of these seed-borne fungal pathogens against oat seed were conducted in the laboratory, and the results showed that the seed germination rate, germination potential, germination index, and vigor index of B2 and B7 seeds were significantly decreased by Alternaria spp., Bipolaris spp., C. allicinum, Pyrenophora sp., Fusarium spp., and N. oryzae (P < 0.05). The plumule length, radicle length, and dry weight of B2 and B7 seedlings were significantly reduced by Bipolaris spp. and Fusarium spp. (P < 0.05). Seed-borne fungal infection might inhibit seed germination and seedling growth of oat.
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The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/supplementary material.
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Funding
This study was supported by Gansu Provincial Science and Technology Major Projects (No.19ZD2NA002) and the China Agriculture Research System (CARS-07-C, Oat). JFW is thankful for the support from USDA-NIFA Multistate Project W4147 and the New Jersey Agricultural Experiment Station.
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CJ Li conceived and designed the experiments. JJ Wang and YP Zhou performed the experiments and wrote the manuscript. LH Xue and XK Wei performed the experiments. JF White and TX Chen revised the manuscript. All authors contributed to the article and approved the submitted version.
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Wang, J., Zhou, Y., Xue, L. et al. Seed-borne fungi associated with oat seeds and their effect on seed germination and seedling growth. J Plant Pathol 105, 225–236 (2023). https://doi.org/10.1007/s42161-022-01270-4
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DOI: https://doi.org/10.1007/s42161-022-01270-4